We used the Flow Cellect Mito Damage Kit and Annexin V-PE Apoptosis Detection Kit obtained from EMD Millipore in the United States. To perform the flow cytometric analysis and understand how cells responded to HAMLET, we seeded 100,000 to 130,000 cells per well. After one day, we treated these cells with different concentrations of HAMLET for 6 h. We then detached the cells, stained them with Annexin V-PE and 7-AAD, and analyzed them using the Guava Personal Cell Analysis Flow Cytometer and CytoSoft software (version 2.1.4; Guava; EMD Millipore, Burlington, MA, USA).
Annexin 5 pe apoptosis detection kit
Manufactured by Merck Group
Sourced in United States
The Annexin V-PE Apoptosis Detection Kit is a laboratory reagent used for the detection and quantification of apoptosis in cell populations. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a cell surface marker of apoptosis. Annexin V is conjugated to the fluorescent dye phycoerythrin (PE) to allow for flow cytometric analysis of apoptotic cells.
Automatically generated - may contain errors
3 protocols using annexin 5 pe apoptosis detection kit
1
HAMLET-Induced Apoptosis Analysis
2
Apoptosis Analysis of A549 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 1 × 105 A549 cells was seeded in a 24-well plate and grown until 80% confluence. Four hundred microliters of CG-M, CG-Mblank, celastrol + G Rh2, celastrol (20 μM), or G Rh2 (8 μM) solutions were added to each well. After 8 h of incubation, the cells were rinsed thoroughly with ice-cold PBS and digested with 200 μL of trypsin to harvest the cell suspension, which was then transferred (50 μL) to a 96-well plate and incubated with an equal volume of Annexin V-PE Apoptosis detection kit (Guava, Merck-Millipore, MA) in the dark for 15 min. Cell apoptosis was measured by flow cytometry (Guava 6HT).
3
Annexin V-PE Apoptosis Assay in A549 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis of the A549 cells at prearranged time interval was determined using an Annexin V-PE apoptosis detection kit (Merck-Millipore) according to the manufacturer’s protocol. Briefly, the cells were trypsinized and then terminated rapidly by phosphate-buffered saline containing 10% fetal bovine serum. Next, 50 μL of the above solution was mixed with 50 μL Annexin V-PE and incubated for 15 minutes. The cells were analyzed immediately by flow cytometry (Guava 6HT; Merck-Millipore).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!