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Be0050

Manufactured by BioXCell
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The BE0050 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor that can accommodate various sample tubes and microplates. The centrifuge is capable of achieving a maximum speed of 6,000 rpm and a maximum relative centrifugal force (RCF) of 3,440 x g.

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Lab products found in correlation

Facsaria iiu, by BD (1 mentions) Be0108, by BioXCell (1 mentions) Be0088, by BioXCell (1 mentions) Be0057, by BioXCell (1 mentions) P1530, by Merck Group (1 mentions)

4 protocols using be0050

1

Antibody-based Immune Cell Analysis

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Anti-IL-10R blocking antibody (BE0050) and isotype IgG1 (BE0088), anti-MHC II antibody (BE0108) were purchased from BioXCell. Fluorescently conjugated antibodies were purchased from Biolegend, eBioscience, and Becton Dickinson. Samples were analyzed using a FACSAria IIu (Becton Dickinson) and data were processed with FlowJo (Treestar).
Chai J.N., Peng Y., Rengarajan S., Solomon B.D., Ai T.L., Shen Z., Perry J.S., Knoop K.A., Tanoue T., Narushima S., Honda K., Elson C.O., Newberry R.D., Stappenbeck T.S., Kau A.L., Peterson D.A., Fox J.G, & Hsieh C.S. (2017). Helicobacter species are potent drivers of colonic T cell responses in homeostasis and inflammation. Science immunology, 2(13), eaal5068.
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2

Muc2 Knockout Colitis Induction

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Muc2+/+ and Muc2–/– littermates received intraperitoneally 500 μg of each antibody: anti-TGF-β (BioXcell, BE0057) and anti-IL-10R (BioXcell, BE0050). Mice were sacrificed 5 days postinjection and colon and serum were collected for colitis inflammatory parameters assessment. Mice were weighted every 24 hours and monitored throughout the duration of the experiment.
Leon-Coria A., Kumar M., Workentine M., Moreau F., Surette M, & Chadee K. (2020). Muc2 Mucin and Nonmucin Microbiota Confer Distinct Innate Host Defense in Disease Susceptibility and Colonic Injury. Cellular and Molecular Gastroenterology and Hepatology, 11(1), 77-98.
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3

Salmonella Flagellin Immunization in Mice

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Monomeric flagellin was purified from flagella isolated from Salmonella Typhimurium (SL3201, fljB−) via HPLC, and purity was verified as previously described39 (link),40 (link) . C57BL/6 mice (WT, TLR5/NLRC4 DKO41 (link), μMT42 (link), IL-10 KO43 (link), TCRβ KO44 (link) were maintained at Georgia State University, Atlanta, Georgia, USA under institutionally approved protocols (IACUC # A14033 and A18006). Mice were immunized with either Salmonella Typhimurium-derived flagellin (10 μg; described above), B. subtilis-derived flagellin (10 μg; InvivoGen, tlrl-bsfla), TNF-α (50 μg/kg body weight; R&D Systems, 410-MT-050) or Poly (I:C) (10 μg/kg body weight; Sigma, P1530) through intraperitoneal injections weekly for a total of 10 injections, with control mice being administered vehicle (PBS). For experiments utilizing anti-IL-10R antibody, 1 mg of anti-IL-10R (Bio X Cell, BE0050) antibody was administered through intraperitoneal injections following flagellin immunization weekly for 4 weeks. For DIO model, mice were fed with a 60% kcal from fat diet (Research Diet, D12492) for 4 weeks. Mice were littermates and group-housed. Mice were killed by CO2 inhalation, and colon length, colon weight, spleen weight and adipose weight were measure. Serum, feces, organs, as well as intestinal contents from the duodenum, ileum, and jejunum were collected for downstream analysis.
Tran H.Q., Ley R.E., Gewirtz A.T, & Chassaing B. (2019). Flagellin-elicited adaptive immunity suppresses flagellated microbiota and vaccinates against chronic inflammatory diseases. Nature Communications, 10, 5650.
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4

Monoclonal Antibody Injection in Mice

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Mice received i.p. injection of 1 mg mAb/100 μL/20 g mouse weight (Bio X Cell; catalog BE0050) (34 (link)). Mice were monitored for weight loss and rectal prolapse over 5 weeks, biweekly. Mice were culled if body weight was 20% less than starting weight.
Bauer K.M., Nelson M.C., Tang W.W., Chiaro T.R., Brown D.G., Ghazaryan A., Lee S.H., Weis A.M., Hill J.H., Klag K.A., Tran V.B., Thompson J.W., Ramstead A.G., Monts J.K., Marvin J.E., Alexander M., Voth W.P., Stephens W.Z., Ward D.M., Petrey A.C., Round J.L, & O’Connell R.M. (2022). CD11c+ myeloid cell exosomes reduce intestinal inflammation during colitis. JCI Insight, 7(19), e159469.
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