We used a cotton pad (Shiseido Cleansing Cotton, Shiseido, Japan; No. 79014) uniquely equipped with hydrophilic and hydrophobic layers in an intact sheet to establish a lateral flow channel in our cotton-based diagnostic device. Commercial chromatography paper (Whatman chromatography paper, GE Healthcare Whatman, Springfield Mill, UK; No. 30306132) was used as a test pad for coating reagents and establishing a reaction zone for colorimetric assays. We introduced a plastic substrate (lamination film, MAS, A4, 216 mm × 303 mm), using a laminator, to reinforce our tightly compacted cotton channel and act as the top and bottom substrate for our cotton-based diagnostic device.
Whatman chromatography paper
Manufactured by GE Healthcare
Sourced in United Kingdom
Whatman chromatography paper is a type of absorbent paper used in chromatographic techniques. It is designed to facilitate the separation and analysis of chemical compounds. The paper provides a uniform and consistent surface for the separation process, enabling reliable and reproducible results.
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Lab products found in correlation
5 protocols using whatman chromatography paper
1
Cotton-Based Diagnostic Device Construction
2
Territorial Scent Marking Behavior in Mice
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Mice were simultaneously placed in one compartment of a 30 × 30 × 30 cm box divided by a metal grid. A clear, smooth barrier was placed on top of the grid to prevent escape. Each compartment contained a 14 cm by 29.5 cm sheet of Whatman chromatography paper (3 mm, GE Healthcare UK Limited CAT No 3030‐2221). Each trial lasted 1 hour, at the end of which both mice were removed and the cages cleaned with 70% alcohol wipes. Marked paper was stained with Ninhydrin spray reagent (Sigma‐Aldrich N1286) and scored using a 1 cm2 grid overlay. All squares containing a scent mark were counted and used in a ratio against usable grid (total grid squares minus shredded sections and urine marks covering more than four consecutive squares). These scent marks/urine drops delineate territorial boundaries and contain chemical cues of social status.15 The winner of each encounter possessed the higher ratio of squares containing sent marks to usable grid.
3
Preparation and Administration of 177Lu-octreotate
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177Lu-octreotate (Nuclear Research and Consultancy Group, IDB Holland) was prepared according to the manufacturer’s instructions. The amount of peptide bound 177Lu (> 99%) was measured by instant thin layer chromatography (ITLC), using Whatman Chromatography paper (3 mm, GE Healthcare UK Limited, Amersham, England) and 0.1 M sodium citrate (Labservice AB, Sundsvall, Sweden). Syringes containing 30 MBq 177Lu-octreotate (in 0.1 ml) were prepared and measured with a well-type ionization chamber (CRC-15R; Capintec, New Jersey, USA). Administered activity to each animal was determined from the 177Lu activity in syringe before and after injection.
4
Radiolabeling 177Lu-octreotate for PRRT
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LuMark ® 177 Lu chloride and peptide were obtained from the Nuclear Research and Consultancy Group (IDB Holland, Baarle-Nassau, The Netherlands). Radiolabeling was conducted according to the manufacturer's instructions. Instant thin-layer chromatography (ITLC), using Whatman™ chromatography paper (3 mm, GE Healthcare UK Limited, Amersham, Great Britain) and 0.1 M sodium citrate (Labservice AB, Sundsvall, Sweden) showed that the amount of peptide-bound 177 Lu was higher than 99%. Syringes containing the desired 177 Lu activity (in 0.1 mL) were prepared from the 177 Lu-octreotate solution and measured according to a previously published method [14] (link).
5
Paper-based Electrochemical Device Fabrication
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Paper networks were designed using Autodesk AutoCAD software and fabricated by patterning wax onto Whatman chromatography paper (GE Healthcare Life Sciences, Pittsburgh, PA) using a Xerox ColorQube printer. The paper was heated for 1 min at 100 °C to allow the wax to permeate and form hydrophobic barriers on resolidification. Whatman 1 Chr paper was used to fabricate single-layer devices. SPDs for amperometric measurements were constructed by gluing (Elmer's Glue-All Multi-Purpose Glue) three layers of 1 Chr paper having identical wax patterns on top of a single layer of 3MM Chr paper. On the 3MM Chr paper, wax patterns were printed on the top side, and a uniform layer of wax was printed on the bottom side. During heating, the wax from both sides merged together to form an enclosed channel. All of the devices were stored at room temperature prior to experiments.
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