A-769662 was obtained from Abcam (Cambridge, MA), AICAR was obtained from Cell Signaling Technology (Danvers, MA). Sulfo-NHS-SS-biotin was obtained from Pierce (Thermo Fisher Scientific, Rockford, IL). Antibodies used for immunoblotting were as follows: anti-EGFR from Genetex (Irvine, CA), anti-CHC from Santa Cruz Biotechnology (Santa Cruz, CA), anti-pACC, anti-AMPK (α1/2), anti- actin, and anti-Erk from Cell Signaling Technology (Danvers, MA), and anti-ZNF142 antibodies from Aviva Systems Biology (San Diego, CA). Antibodies used for immunofluorescence microscopy were as follows: anti-β1-integrin from EMD Millipore, Darmstadt, Germany), and anti-TfR from Santa Cruz Biotechnology (Santa Cruz, CA).
Anti egfr
Manufactured by GeneTex
Sourced in United States
Anti-EGFR is a lab equipment product that functions as an antibody specifically targeting the epidermal growth factor receptor (EGFR). It is used in various research applications involving the detection and analysis of EGFR expression in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti tfr, by Santa Cruz Biotechnology (1 mentions)
Anti p acc, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti chc, by Santa Cruz Biotechnology (1 mentions)
Anti β1 integrin, by Merck Group (1 mentions)
Aicar, by Cell Signaling Technology (1 mentions)
A769662, by Abcam (1 mentions)
P akt, by Santa Cruz Biotechnology (1 mentions)
Pkm2 sirna, by Thermo Fisher Scientific (1 mentions)
α tubulin, by Cell Signaling Technology (1 mentions)
E cadherin, by Santa Cruz Biotechnology (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Twist, by GeneTex (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Quercetin, by Merck Group (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Vimentin, by GeneTex (1 mentions)
Anti lrig1, by GeneTex (1 mentions)
Anti ahr, by Santa Cruz Biotechnology (1 mentions)
4 protocols using anti egfr
1
Investigating AMPK Signaling Pathway
2
Erlotinib and Quercetin Pathway Analysis
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Quercetin and other chemicals were purchased from Sigma (St. Louis, MO, USA) unless specified otherwise. Erlotinib (Tarceva, OSI Pharmaceuticals, Melville, NY, USA) was purchased from Lumtec (Hsinchu, Taiwan), antibodies against Akt, E-cadherin, GAPDH, p-Akt, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and anti-p-EGFR antibodies were purchased from Millipore (Burlington, MA, USA). Anti-EGFR, ERK, HK2, LDHA, MMP-2, PKM2, p27, Twist, and Vimentin antibodies were purchased from GeneTex, Inc. (Irvine, CA, USA). Antibodies against GLUT1, p-ERK, N-cadherin, and α-tubulin were obtained from Cell Signaling Technology (Danvers, MA, USA). MMP-9 and p21 antibodies were obtained from Abcan (Cambridge, UK) and Proteintech (Rosemont, IL, USA), respectively. PKM2 siRNA and the Lipofectamine RNAiMAX reagent were purchased from Invitrogen (Grand Island, NY, USA).
3
Immunohistochemical Analysis of AHR, LRIG1, and EGFR
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The lung specimens of wild-type and AHR-KO mice were a gift from Dr. Lee CC [54 (link)]. The human lung disease and normal tissue array were obtained from US Biomax, Inc., Derwood, MD, USA (catalog no. LUD481). The expression patterns of AHR, LRIG1, and EGFR were detected by using specific primary antibodies (anti-AHR, Santa Cruz, catalog no. sc-74571; anti-LRIG1 and anti-EGFR, Genetex, catalog no. GTX119485 and GTX121919, respectively) according to the standard protocol of Bio-Check Laboratories Ltd. (New Taipei City, Taiwan). Peroxidase-labeled specimens were observed on a Nikon light microscope equipped with a Polychrome-III camera (YC technology, New Taipei City, Taiwan) and Image Eye software (FMJ Software, Stockholm, Sweden).
4
Spheroid Proteome Analysis of BJ Extract
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The spheroids were formed by plating 1 × 106 cells per well in serum-free media with supplementation of growth factors and treated with various concentrations of aqueous extractions of BJ (0, 5, 10, and 15 mg/mL) for 12 h. The harvested cell pellets were lysed and the protein concentrations determined using a Bio-Rad protein assay kit for (Hercules, CA, USA) before being resolved by electrophoresis and transferred to nitrocellulose membrane. The blots were blocked with 5% non-fat milk and incubated with 1:2000 dilutions of primary antibodies, including anti-pAkt (GTX128414); anti-PARP (GTX112864); anti-EGFR (GTX121919); anti-pEGFR (GTX61507), anti-ABCG2 (GTX100437), anti-Nanog (GTX100863), anti-CD133 (GTX100567), anti-Sox2 (GTX627405), and anti-ALDH1A1 (GTX123973), from GeneTex. Membranes were then incubated with 0.3 µg/mL of peroxidase-conjugate anti-mouse or anti-rabbit IgG (Thermo Fisher Scientific) and detected with enhanced chemiluminescence substrate (Thermo Fisher Scientific). The loading control was incubated with anti-GAPDH antibody (GTX100118, GeneTex). The signals were visualized with enhanced LAS-4000 (FUJIFILM) apparatus and the band intensities of images analyzed using ImageJ software.
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