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Ldl c

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The LDL-C is a laboratory instrument used to measure the concentration of low-density lipoprotein cholesterol (LDL-C) in a blood sample. It is a core function of the equipment to provide an accurate assessment of the LDL-C levels.

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Lab products found in correlation

Hdl c, by Nanjing Jiancheng (18 mentions) Mcp 1, by Jiangsu Meimian (3 mentions) T cho, by Nanjing Jiancheng (2 mentions) Oil red o, by Merck Group (2 mentions) Gsh px, by Nanjing Jiancheng (2 mentions) Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Antibodies against srebp 1c, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin antibody, by Abcam (1 mentions) Malonaldehyde, by Nanjing Jiancheng (1 mentions) Rabbit anti pparγ, by Bioss Antibodies (1 mentions) Radioimmunoprecipitation assay ripa lysate buffer, by MultiSciences Biotech (1 mentions) Bicinchoninic acid bca protein quantitative kit, by MultiSciences Biotech (1 mentions) Sds polyacrylamide gel electrophoresis sds page preparation kit, by MultiSciences Biotech (1 mentions) Hdl c, by Hitachi (1 mentions) Ldl c, by Hitachi (1 mentions) Srebp 1, by ABclonal (1 mentions) β actin, by ABclonal (1 mentions) Glutathione (gsh), by Nanjing Jiancheng (1 mentions) Cholestyramine, by Merck Group (1 mentions) Pancreatic lipase, by Solarbio (1 mentions)

Spelling variants of this product

Low-density lipoprotein cholesterol (LDL-C) (6 citations) LDL-C assay kit (4 citations) Low-density lipoprotein cholesterol (LDL-C) assay kits (2 citations) HDL-C and LDL-C assay kits (2 citations)

26 protocols using ldl c

1

Tilapia Metabolic Regulation Study

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Tilapias were purchased from Hainan Xiangtai Fishery Co., LTD (Hainan, China) and stored at −20 °C. High-fat feed was purchased from Hunan Slac Jingda Laboratory Animal Co., Ltd. (Changsha, China). Enzyme-linked immunosorbent assay kits for insulin (INS), adiponectin (ADPN), leptin (LEP), IL-6, tumor necrosis factor-alpha (TNF-α) and free fatty acids (FFAs) were acquired from Shanghai Enzyme-linked Biotechnology Inc. (Shanghai, China). Commercial kits for aminotransferase (ALT), aminotransferase (AST), TC, TG, HDL-C, LDL-C, glutathione (GSH), superoxide dismutase (SOD) and malonaldehyde (MDA) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies against SREBP-1c were obtained from Santa Cruz Biotechnology Inc. (CA, USA). Antibodies against AMP-activated protein kinase (AMPK), phospho-AMPK, peroxisome proliferator-activated receptor (PPARγ) and fatty acid synthase (FAS) were obtained from Cell Signaling Technology (Beverly, MA, USA). Mouse anti-β-actin antibody and goat anti-mouse lgG-HRP secondary antibody were obtained from Abcam Inc. (Cambridge, UK). All other chemicals and reagents used were of analytical grade.
Yu H., Yi X., Gao X., Ji J., Liu Z., Xia G., Li C., Zhang X, & Shen X. (2022). Tilapia-Head Chondroitin Sulfate Protects against Nonalcoholic Fatty Liver Disease via Modulating the Gut–Liver Axis in High-Fat-Diet-Fed C57BL/6 Mice. Foods, 11(7), 922.
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2

Serum Biomarker Measurement in Mice

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Serum indices in mice were measured using the following ELISA kits: 25(OH)D (Immunodiagnosticsystems, Boldon, UK, AC-57SF1), parathyroid hormone (PTH, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, H207), and insulin (INS, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, H203-1-2). Kits used to measure other serum indices were as follows: calcium (Ca, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, C004-2-1), phosphorus (P, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, C006-1-1), low-density lipoprotein cholesterol (LDL-C, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, A113-1-1), high-density lipoprotein cholesterol (HDL-C, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, A112-1-1), total cholesterol (TC, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, A111-1-1), and triacylglycerol (TG, Nanjing Jiancheng Bioengineering Institute, Nanjing, China, A110-1-1).
Cui X., Wang K., Zhang J, & Cao Z.B. (2023). Aerobic Exercise Ameliorates Myocardial Fibrosis via Affecting Vitamin D Receptor and Transforming Growth Factor-β1 Signaling in Vitamin D-Deficient Mice. Nutrients, 15(3), 741.
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3

Plasma Lipid and Liver Enzyme Analysis

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Mice were sacrificed by CO2 inhalation, and plasma was collected to detect total cholesterol (TC), triglyceride (TG; Stanbio Laboratory, Boerne, TX, USA), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) levels by corresponding commercial test kits. Subsequently, plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed colorimetrically utilizing assay test kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
Zhou Y., Wang Y., Vong C.T., Zhu Y., Xu B., Ruan C.C., Wang Y, & Cheang W.S. (2022). Jatrorrhizine Improves Endothelial Function in Diabetes and Obesity through Suppression of Endoplasmic Reticulum Stress. International Journal of Molecular Sciences, 23(20), 12064.
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4

Mouse Blood Lipid Profile Analysis

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Mouse peripheral blood was collected and left to stand at 4°C for 4 h. The blood wasthen centrifuged at 10,000r/min for 10 min at 4°C, and the supernatant was collected. TC, TG, HDL-C, and LDL-C (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) in serum were detected with a kit according to the manufacturer'sdirections.
Liu J., Lai L., Lin J., Zheng J., Nie X., Zhu X., Xue J, & Liu T. (2020). Ranitidine and finasteride inhibit the synthesis and release of trimethylamine N-oxide and mitigates its cardiovascular and renal damage through modulating gut microbiota. International Journal of Biological Sciences, 16(5), 790-802.
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5

Lipid Metabolism and Antioxidant Evaluation

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The total cholesterol (T-CHO), triglycerides (TG), high-density lipoprotein (HDL) cholesterol (HDL-C), low-density lipoprotein (LDL) cholesterol (LDL-C), superoxide dismutase (SOD), malondialdehyde (MDA), tripeptide glutathione (GSH), and coomassie bright blue (CBB) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); rabbit anti-PPARγ (proliferator-activated receptor γ) and rabbit anti-APOE (peroxisome apolipoprotein E) antibodies were products of Bioss Antibodies (Beijing, China); GAPDH antibodies, radioimmunoprecipitation assay (RIPA) lysate buffer, bicinchoninic acid (BCA) protein quantitative kit, and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) preparation kit were purchased from Multi Sciences (Hangzhou, China); RNA TRIzol Reagent was purchased from Servicebio Company (Wuhan, China); the RevertAid First Strand cDNA Synthesis Kit was purchased from Thermo Fisher (MO, USA); and the all other chemicals used in this study were of analytical reagent grade.
Wang L., Fan W., Zhang M., Zhang Q., Li L., Wang J., Zhu L., Wei D., Peng W, & Wu C. (2019). Antiobesity, Regulation of Lipid Metabolism, and Attenuation of Liver Oxidative Stress Effects of Hydroxy-α-sanshool Isolated from Zanthoxylum bungeanum on High-Fat Diet-Induced Hyperlipidemic Rats. Oxidative Medicine and Cellular Longevity, 2019, 5852494.
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6

Serum Lipid Profile Analysis

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The serum samples were collected from the fresh whole blood at room temperature for 1 h, then centrifuged at 2000 g for 15 min and stored at −70°C until further analysis. Blood physiological indexes of serum samples, including the low-density lipoprotein cholesterol (LDL-c) (Jiancheng, Corp., Nanjing, China) and high-density lipoprotein cholesterol (HDL-c) (Jiancheng), total cholesterol (TC) (Jiancheng), and triglyceride (TG) (Jiancheng), were determined using a blood analyzer (Hitachi 7100), while the very low-density lipoprotein cholesterol (VLDL-c) could instead be determined by the cholesterol contained in the VLDL and was indirectly determined by the Friedewald formula:VLDL-c = TC-HDL-c-LDL-c (Friedewald et al., 1972 (link); Delong et al., 1986 (link)).
Li H., Li Y., Yang L., Zhang D., Liu Z., Wang Y., Han R., Li G., Li Z., Tian Y., Kang X, & Liu X. (2020). Identification of a Novel Lipid Metabolism-Associated Hepatic Gene Family Induced by Estrogen via ERα in Chicken (Gallus gallus). Frontiers in Genetics, 11, 271.
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7

Orlistat and Lipid Biomarkers Analysis

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OA (HPLC ≥ 98 % ) was supplied from Yuanye (Shanghai, China) and dissolved by 2 % Tween 80 phosphate-buffered saline (PBS pH:7.2-7.4). Orlistat capsules were purchased from Aili (Chongqing, China). The kits of TC, TG, HDL-C, LDL-C, ALT, AST, GSH, and MDA were obtained from Nanjing Jiancheng Biotechnology Co. (Nanjing, Jiangsu, China). The enzyme-linked immunosorbent assay (ELISA) kits of interleukin 6 (IL-6), tumor necrosis factor- α (TNF- α ), Glucagon-Like Peptide 1 (GLP-1) and Monocyte Chemotactic Protein 1 (MCP-1) was supplied from Meimian (Yancheng, Jiangsu, China). The primary antibodies of SREBP-1, TGR5, UCP-1, and β -actin were purchased from Abclonal (Wuhan, Hubei, China). Meanwhile, Abcam (Cambridge, UK) provides the secondary antibodies and PPAR γ antibody.
Liu T., Wang J., Tong Y., Wu L., Xie Y., He P., Lin S, & Hu X. (2024). Integrating network pharmacology and animal experimental validation to investigate the action mechanism of oleanolic acid in obesity. Journal of Translational Medicine, 22, 86.
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8

Liver Tissue Biochemical Profiling

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High-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), alanine aminotransferase (ALT), free fatty acid (FFA), and asparagus transaminase (AST) detection kits were purchased from Nanjing Jiancheng Institute of Bioengineering Co., Ltd. 10% tissue homogenate was prepared from liver tissue with normal saline, and the above-mentioned biochemical indicators of liver tissues homogenate and serum were measured according to the manufacturer’s instructions.
Wen S., An R., Li Z.G., Lai Z.X., Li D.L., Cao J.X., Chen R.H., Zhang W.J., Li Q.H., Lai X.F., Sun S.L, & Sun L.L. (2022). Citrus maxima and tea regulate AMPK signaling pathway to retard the progress of nonalcoholic fatty liver disease. Food & Nutrition Research, 66, 10.29219/fnr.v66.7652.
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9

Serum Lipid and LPS Profiling

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Blood was collected from the femoral artery in centrifuge tubes. After 2 h at room temperature, the blood was centrifuged at 2 °C – 8 °C for 15 min at 3000 rpm. The supernatant was removed and frozen at −80 °C. Enzyme-linked immunosorbent assay (ELISA) kits were used for the determination of total cholesterol (TC; Nanjing Jiancheng Biotechnology Co.,Ltd., Nanjing, CHN; A111-1-1), triglyceride (TG; Nanjing Jiancheng Biotechnology Co.,Ltd., Nanjing, CHN; A110-1-1), high-density lipoprotein (HDL-C; Nanjing Jiancheng Biotechnology Co.,Ltd., Nanjing, CHN; A112-1-1), low-density lipoprotein (LDL-C; Nanjing Jiancheng Biotechnology Co.,Ltd., Nanjing, CHN; A113-1-1), and lipopolysaccharide (LPS; Nanjing Jiancheng Biotechnology Co.,Ltd., Nanjing, CHN; SU-B31044) in serum. The procedure followed was as follows: the sample was added, followed by enzyme conjugates; then, the chromogenic substrate was added, and the reaction was stopped. The optical density was measured at 510 nm on an enzyme labeling instrument (Perlong; Beijing, CHN; DNM9602G) [41 (link)].
Wen L., Xiong W., Wei G., Zhang L., Liu Y., Zhang T., Altamirano A., Yin Q., Zhang T, & Yan Z. (2022). Differential Response of Ileal and Colonic Microbiota in Rats with High-Fat Diet-Induced Atherosclerosis. International Journal of Molecular Sciences, 23(19), 11154.
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10

Biochemical Marker Quantification Protocol

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The concentrations of total cholesterol (T-CHO; code No. A111-2-1), triglyceride (TG; code No. A110-2-1), creatinine (Cr; code No. C011-1-1), low-density lipoprotein cholesterol (LDL-C; code No. A113-1-1), and high-density lipoprotein cholesterol (HDL-C; code No. A112-1-1) were determined using commercial kits purchased from Jiancheng Bioengineering Institute (Nanjing, China). All procedures were strictly completed in accordance with reagent instructions.
Tian X., Lu Q., Zhao S., Li J., Luo Q., Wang X., Zhang Y, & Zheng N. (2021). Purple Corn Anthocyanin Affects Lipid Mechanism, Flavor Compound Profiles, and Related Gene Expression of Longissimus Thoracis et Lumborum Muscle in Goats. Animals : an Open Access Journal from MDPI, 11(8), 2407.
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