Cyclin b1

Manufactured by Cell Signaling Technology

Sourced in United States, United Kingdom, China

Cyclin B1 is a protein that plays a crucial role in the regulation of the cell cycle. It is a key component of the M-phase promoting factor (MPF), which is responsible for driving cells from the G2 phase into the M phase of the cell cycle. Cyclin B1 is expressed during the late G2 and M phases of the cell cycle and is degraded at the end of mitosis.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-cyclin B1 (82 citations) Rabbit anti-cyclin B1 (7 citations) Mouse anti-Cyclin B1 (6 citations) Cyclin B1 antibody (5 citations) Cyclin B1 (4138) (3 citations) Rabbit Cyclin B1 XP (2 citations) Rabbit anti-Cyclin B1 (4138S; (2 citations) Mouse monoclonal anti-cyclin B1 (2 citations) Cyclin B1 (12231) (2 citations) Cyclin B1 (4138P), (2 citations)

294 protocols using cyclin b1

Apoptosis and Cell Cycle Regulation Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were all obtained from Cell Signaling Technology; catalog numbers are in parentheses: caspase 3 (9662), caspase 8 (9746), cleaved caspase 9 (9501), apoptosis-inducing factor (4642), cyclin-dependent kinase (CDK) 1 (9112), CDK2 (2546), CDK4 (2906), cyclin B1 (4135), cyclin D1 (2926), cyclin E1 (4129), p21 (2947), p27 (3686), p53 (9282), checkpoint kinase 2 (CHEK2) (6334), ataxia telangiectasia mutated (ATM) protein kinase (2873), phospho-histone H3 (pH3; Ser10) (9701), and phospho-histone H2A variant X (γ-H2AX) (9718). Horseradish peroxidase-conjugated secondary antibodies for western blot were from Santa Cruz Biotechnology (sc-2357). Alexafluor 488-conjugated secondary antibodies for immunofluorescence were from Jackson ImmunoResearch (211-545-109). Protease inhibitor cocktail (539131) and phosphatase inhibitor cocktail (524625) were both from Calbiochem. Annexin V (A13201) was from Thermo Fisher. Etoposide (GR-307) was from BioMol. Muse^® cell analyzer kits for measurement of caspase3/7 (MCH100108) and cell cycle (MCH100106) were from EMD Millipore. Unless otherwise noted, protein was measured by the method of Lowry et al. (9 (link)). Statistical significance was determined using Student’s t-test.

Yang H., Shi X., Kolar E.A., Clay E.M., Xia S., Pei Z, & Watkins P.A. (2023). VERY LONG-CHAIN ACYL-CoA SYNTHETASE-3 (ACSVL3) PROMOTES THE MALIGNANT GROWTH BEHAVIOR OF U87 GLIOMA CELLS VIA CHANGES IN CELL CYCLE WITHOUT AFFECTING APOPTOSIS. bioRxiv.

+ Open protocol

+ Expand

Western Blotting Analysis of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were harvested and lysed in RIPA buffer (Cell Signalling Technology, Danvers, MA), and the resultant proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PolyScreen membranes (NEN, Boston, MA, USA). The membranes were blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.05% Tween-20 (TBS-T) and probed with antibodies targeting the following proteins: β-catenin (Cell Signalling Technology), cyclin D1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), AXIN2, c-Myc, STAT3, cyclin B1, Gab1, c-caspase-3, p-ERK (Cell Signalling Technology), HA, β-actin, and γ-tubulin (Santa Cruz Biotechnology). Primary antibodies were detected with HRP-conjugated anti-mouse or anti-rabbit antibodies, as appropriate, that were subjected to enhanced chemiluminescence (Amersham, Buckinghamshire, UK).

Moon J.H., Hong S.W., Kim J.E., Shin J.S., Kim J.S., Jung S.A., Ha S.H., Lee S., Kim J., Lee D.H., Park Y.S., Kim D.M., Park S.S., Hong J.K., Kim D.Y., Kim E.H., Jung J., Kim M.J., Kim S.M., Deming D.A., Kim K., Kim T.W, & Jin D.H. (2019). Targeting β-catenin overcomes MEK inhibition resistance in colon cancer with KRAS and PIK3CA mutations. British Journal of Cancer, 120(9), 941-951.

+ Open protocol

+ Expand

Atorvastatin Inhibits Proliferation and Induces Apoptosis in Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Atorvastatin calcium, Mevalonic acid, GGPP, FPP, Propidium iodide (PI) and 2′,7′-dichlorofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was from Amresco (Solon, OH, USA). 5,5′,6,6′-tetrachloro-1, 1′,3,3′-tetraethylbenzimidazole-carbocyanide iodine (JC-1) and cell mitochondria isolation kit were obtained from Beyotime Biotechnology (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit, as well as antibodies against p-pRb (S780) (1:1,000), p27 (1:1,000) and cyclinD1 (1:1,000) were from BD Biosciences Pharmingen (San Jose, CA, USA). Antibodies against pRb (1:1,000), cyclinB1 (1:2,000), cdc2 (1:1,000), caspase-3 (1:1,000), caspase-9 (1:1,000), poly (ADP-ribose) polymerase (PARP) (1:1,000), Cytochrome c (1:1,000), YAP (1:1,000), p-YAP (Ser127) (1:1,000), Rho A (1:1,000), β-actin (1:1,000), anti-mouse (1:2,000), anti-rabbit HRP-conjugated and anti-rabbit Alexa Fluor 488-conjugated secondary antibodies (1:2,000) were from Cell Signaling Technology (Danvers, MA, USA). Antibodies against Bcl-2 (1:1,000), Bax (1:1,000) and Lamin B (1:500) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Zhang L., Chen T., Dou Y., Zhang S., Liu H., Khishignyam T., Li X., Zuo D., Zhang Z., Jin M., Wang R., Qiu Y., Zhong Y, & Kong D. (2019). Atorvastatin Exerts Antileukemia Activity via Inhibiting Mevalonate-YAP Axis in K562 and HL60 Cells. Frontiers in Oncology, 9, 1032.

+ Open protocol

+ Expand

Signaling Pathways in Cell Cycle Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chemicals used in this study were crizotinib (Selleck Chemicals LLC, Houston, TX, USA), Cyclosporine A (CsA) (J&K chemical Ltd., Beijing, China), PD98059 (Selleck Chemicals LLC, Houston, TX, USA), MK-2206 (Selleck Chemicals LLC, Houston, TX, USA). The primary antibodies against phospho-Erk1/2, Erk1/2, phospho-AKT, AKT, phospho-STAT3, STAT3, phospho-Cdc25c, phospho-CDK1, CDK1, Cyclin B1, Bcl-XL, caspase-3 and PARP were purchased from Cell Signaling Technology (Boston, MA, USA). KSR2 and Cdc25c were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). The secondary antibodies were horse radish peroxidase (HRP)-conjugated anti-rabbit IgG, anti-mouse IgG (Cell Signaling Technology).

Liu Z., Jiang L., Li Y., Xie B., Xie J., Wang Z., Zhou X., Jiang H., Fang Y., Pan H, & Han W. (2019). Cyclosporine A sensitizes lung cancer cells to crizotinib through inhibition of the Ca2+/calcineurin/Erk pathway. EBioMedicine, 42, 326-339.

+ Open protocol

+ Expand

Western Blot Analysis of Stress Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted with cell lysis buffer containing 1% Triton X-100, sonicated using QSonica-Q800R2, quantified using bicinchoninic acid assay (Sigma) and separated on NuPAGE™ 4-12% Bis-Tris precast gels (Thermo Fisher). Immunoblot analyses were conducted according to standard protocol with the following antisera: caspase-3 (#9662), PARP (#9542), β-actin (#3770), IRE1α (#3294), p-eIF2α (#3597), t-eIF2α (#2103), p-P38 MAPK (#4511), t-P38 MAPK (#8690), p-JNK1/2 (#4668), t-JNK1/2 (#9252), p-ERK1/2 (#9101), t-ERK1/2 (#9102), CHOP (#5554), Bim (#2933), Bcl-2 (#4223), Bax (#5023), p-Akt (#4060), t-Akt (#9272), GAPDH (#5174), cyclin b1 (#12231), p-CDK1 substrates (#9477) and FLAG (#14793) from Cell Signaling Technology; p62 (#ab56416) and Mcl-1 (#ab31948) antibodies were purchased from Abcam, while LC3 antibody (#NB100-2220) was obtained from Novus Biologicals. Images were captured using a ChemiDoc™ Touch Imaging System and processed using ImageLab™ Software.

Trapika I.G., Liu X.T., Chung L.H., Lai F., Xie C., Zhao Y., Cui S., Chen J., Tran C., Wang Q., Zhang S., Don A.S., Li G.Q., Hanrahan J.R, & Qi Y. (2021). Ceramide Regulates Anti-Tumor Mechanisms of Erianin in Androgen-Sensitive and Castration-Resistant Prostate Cancers. Frontiers in Oncology, 11, 738078.

+ Open protocol

+ Expand

Western Blot Analysis of AKT-mTOR and Cell Cycle Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The expression of AKT-mTOR proteins (AKT, mTOR, phospho-AKT, phospho-mTOR, p70S6K, and phospho-p70S6K) and cell cycle-related proteins (P21, cyclinB1, and CDK1/2) were analyzed by western blots. Western blot was applied as described above to detect GOLPH3 protein expression in the four PCa cell lines. Primary antibodies AKT, mTOR, phospho-AKT, phospho-mTOR, p70S6K, phospho-p70S6K, P21, cyclinB1, and CDK1/2 were purchased from Cell Signaling Technology (CST, Beverly, MA) and Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) and used at a concentration of 1: 1000, 1: 500 and 1: 200, respectively.

Li W., Guo F., Gu M., Wang G., He X., Zhou J., Peng Y., Wang Z, & Wang X. (2015). Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer. Journal of Cancer, 6(5), 420-429.

+ Open protocol

+ Expand

Western Blot Analysis of Cell Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols

Treated cells were lysed with RIPA Lysis Buffer (P0013, Beyotime, China). The total protein quality was determined by BCA Protein Assay Kit (Pierce; Rockford IL, USA). The protein were denatured and separated by 10 % SDS-PAGE and then electrotransferred to polyvinylidene difluoride membranes (Immobilon PVDF, Millipore, Bedford). The membranes were washed with Tris-Buffered Saline and Tween 20 (PBST) and then blocked for 2 h at room temperature. The membranes were then incubated with special antibodies against β-tubulin, caspase3, PARP, CyclinB1, Cdc25c, Cdc2 (Cell Signaling Technology, USA) at 4 °C overnight and washed with TBST. Later, the membranes were incubated with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (1:5000) for 1 h at room temperature and then washed with TBST. Protein bands were detected by an enhanced chemiluminescence detection system (Westar Supernova, Cyanagen, Italy) and recorded on X-ray film (Fujifilm; Tokyo, Japan)

Fang S., Chen L., Yu M., Cheng B., Lin Y., Morris-Natschke S.L., Lee K.H., Gu Q, & Xu J. (2015). Synthesis, Antitumor Activity, and Mechanism of Action of 6-Acrylic Phenethyl Ester-2-pyranone Derivatives. Organic & biomolecular chemistry, 13(16), 4714-4726.

+ Open protocol

+ Expand

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Abs against phospho-ATM (Ser1981), ATM, phospho-Chk1 (Ser317), Chk1, phospho-Chk2 (Thr68), Chk2, phospho-p53 (Ser15), PTEN, phospho-CDK2 (Thr160), CDK2, Cyclin E, Cyclin B1, CDC2, phospho-CDC2 (Tyr15), cleaved PARP (Asp214), phospho-STAT3 (Ser727), STAT3, STAT5, caspase-3 and active caspase-3 (Asp175) were all obtained from Cell Signaling (Danvers, MA, USA). Abs recognizing p53 (EMD Millipore, Darmstadt, Germany), p21 (Santa Cruz, Dallas, Texas) and phospho-STAT5 (Ser726/731) (Sabbiotech, College Park, MD, USA) were obtained as indicated.

Chen L.M., Liu P.Y., Chen Y.A., Tseng H.Y., Shen P.C., Hwang P.A, & Hsu H.L. (2017). Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression. Scientific Reports, 7, 11864.

+ Open protocol

+ Expand

Ovarian Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The platinum-resistant human ovarian cancer cell line A2780/CP70 (p53 wild-type) was a generous gift from Dr B. Jiang at West Virginia University. IOSE-364, a normal ovarian surface epithelial cell line, was presented by Dr N. Auersperg at University of British Columbia, Canada. The cells were cultured in RPMI-1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Invitrogen) at 37°C in a humidified incubator with 5% CO₂. Theaflavin-3, 3′-digallate (TF3, >90.0%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibodies against Bcl-xL, Bad, p21, p53, MDM2 and GAPDH were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The primary antibodies against caspase-8 and -9, Puma, Bax, cyclin B1, phospho-cdc2 (Tyr15), cdc2, DR5, FADD, phospho-Akt (Thr180/Tyr182) and total-Akt were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

TU Y., KIM E., GAO Y., RANKIN G.O., LI B, & CHEN Y.C. (2016). Theaflavin-3, 3′-digallate induces apoptosis and G2 cell cycle arrest through the Akt/MDM2/p53 pathway in cisplatin-resistant ovarian cancer A2780/CP70 cells. International Journal of Oncology, 48(6), 2657-2665.

+ Open protocol

+ Expand

Molecular Mechanism Regulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-Hydroxyphthalimide was purchased from Accela ChemBio Co., Ltd. (Shanghai, China). Propidium iodide (PI), RNase A and 4,6-diamidino-2-phenylindole (DAPI) were from Sigma-Aldrich. Antibodies of mTOR, Phospho-mTOR (Ser2448), Phospho-mTOR (Ser2481), S6K1, Phospho-S6K1 (Thr389), Phospho-S6 Ribosomal Protein (Ser235/236), 4E-BP1, Phospho-4E-BP1 (Ser65), Phospho-Akt (Ser473), Phospho-Akt (Thr308), Cleaved PARP, Cleaved Caspase 3, Caspase 9, cyclin B1, cdc2 were obtained from Cell Signaling Technology; antibodies of S6, Akt, P-ERK1/2, β-actin, Caspase 3, Caspase 8, Bcl-xL, survivin were from Santa Cruz; antibody of ERK was from Epitomics; antibody of eIF4E was obtained from BD Biosciences; Alexa Fluor® 647 donkey anti-mouse IgG antibody was purchased from Invitrogen; all the other secondary antibodies were from Sigma-Aldrich.

Wang M., Zhou A., An T., Kong L., Yu C., Liu J., Xia C., Zhou H, & Li Y. (2016). N-Hydroxyphthalimide exhibits antitumor activity by suppressing mTOR signaling pathway in BT-20 and LoVo cells. Journal of Experimental & Clinical Cancer Research : CR, 35, 41.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!