Tissue microarrays (TMAs) had already been prepared for previous studies [4 (link), 25 (link)], by taking three cores of 0.6 mm from formalin-fixed paraffin-embedded (FFPE) tissue blocks. Of these blocks, 4-μm tissue sections were cut and then immunohistochemically stained for PD-1 and PD-L1 with the Ventana autostainer (Roche, Tuscon, AZ, USA), according to the instructions of the manufacturer. For PD-1, we used the mouse anti-PD-1 monoclonal antibody (ab52587/ NAT105, dilution 1:50; Abcam, Cambridge, UK) and for PD-L1, a rabbit anti-PD-L1 monoclonal antibody [741–4905 (clone sp263, dilution Ventana ready to use; Ventana Medical Systems, Tuscon, AZ, USA)]. ER (Ventana 1:100), PR (Dako 1:100), and HER2 (Neomarkers 1:100) were also stained with the Ventana autostainer.
741 4905 clone sp263
Manufactured by Roche
Sourced in United Kingdom
The 741–4905 (clone sp263) is a lab equipment product manufactured by Roche. It is designed for use in various laboratory applications. The core function of this product is to provide a reliable and consistent tool for research and analysis tasks. No further details or interpretations about its intended use are provided.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 741 4905 clone sp263
1
Immunohistochemical Profiling of PD-1 and PD-L1
2
Immunohistochemical Profiling of Tumor Samples
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The TMAs contained three cores of 0.6 mm from representative areas in formalin-fixed paraffin-embedded (FFPE) tissue blocks of both primary tumor and metastasis. 4 µm thick tissue sections were cut and immunohistochemically stained with the Ventana autostainer (Roche, Tuscon, USA). For PD-1, we used a mouse anti-PD-1 monoclonal antibody (ab52587 (NAT105, dilution 1:50, Abcam, Cambridge, UK)) and for PD-L1, a rabbit anti-PD-L1 monoclonal antibody (741–4905 (clone sp263, dilution Ventana ready to use; Ventana Medical Systems, Tuscon, Arizona, USA)). ER (Ventana 1:100), PR (Dako 1:100) and HER2 (Neomarkers 1:100) were also stained with the Ventana autostainer.
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