Human NSCLC A549 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (v/v) fetal bovine serum (FBS) and penicillin–streptomycin (100 U/mL; 100 µg/mL) at 37°C in a 5% CO2 humidified incubator. DMEM, FBS, and penicillin–streptomycin were purchased from Maixin Bio (Fuzhou, China).
Dulbecco modified eagle medium (dmem)
Manufactured by Maixin Group
Sourced in China
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium commonly used in the laboratory setting. It provides essential nutrients and growth factors required for the maintenance and proliferation of various cell lines. DMEM supports the in vitro cultivation of a wide range of cell types, including adherent and suspension cultures.
Automatically generated - may contain errors
2 protocols using dulbecco modified eagle medium (dmem)
1
NSCLC A549 Cell Culture Protocol
2
Culturing Human Ovarian Cancer Cells
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The human ovarian cancer cell lines, ES2, OVCAR429, HEY, OVCAR433, OVCAR5, SK-OV3, OCC1, OVCAR3, TOV21G, HEC1A, IGROV1, A2780, and OVCAR4 were provided by the Department of Biochemistry and Molecular Biology, Georgia Regents University (Georgia, USA) as a kind gift. Cells were cultured in Dulbecco's modified Eagle’s medium (DMEM) supplied with 10 % (w/v) fetal bovine serum (FBS) at 37 °C in a humidified incubator containing 5 % CO2. LPA was purchased from Avantis Lipid (Alabaster, AL). DMEM, serum, and other cell culture supplies were purchased from Maixin Biotechnology (Fuzhou, China).
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