400 spectrometer

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 400 spectrometer is a laboratory instrument designed for spectroscopic analysis. It is capable of measuring and analyzing the absorption, emission, or scattering of electromagnetic radiation by various samples.

Automatically generated - may contain errors

Lab products found in correlation

Lambda 25 uv vis spectrometer, by PerkinElmer (4 mentions) Vertex 70 ftir spectrometer, by Bruker (3 mentions) B 535 apparatus, by Büchi (2 mentions) Orbitrap exploris 240 mass spectrometer, by Thermo Fisher Scientific (1 mentions) Cisplatin, by Merck Group (1 mentions) Synergy h1 hybrid reader, by Agilent Technologies (1 mentions) Em 208, by Philips (1 mentions) 1290 uhplc system, by Agilent Technologies (1 mentions) Quemesa, by EMSIS (1 mentions) 241 polarimeter, by PerkinElmer (1 mentions) 2100 microscope, by JEOL (1 mentions) 2550 spectrophotometer, by Shimadzu (1 mentions) Jsm 7600f microscope, by JEOL (1 mentions) Differential scanning calorimeter, by Mettler Toledo (1 mentions) Thermogravimetric analyzer, by Mettler Toledo (1 mentions) Mpms xl7 squid magnetometer, by Quantum Design (1 mentions) Silica gel 60, by Merck Group (1 mentions) 341 polarimeter, by PerkinElmer (1 mentions) Lh 20, by Merck Group (1 mentions) Ftir spectrophotometer, by PerkinElmer (1 mentions)

Close spelling variants of this product

Inova 400 MHz spectrometer (37 citations) Mercury 400 NMR spectrometer (15 citations) Unity 400 spectrometer (13 citations) Spectrometers (10 citations) MR 400 MHz spectrometer (10 citations) Mercury 400 MHz NMR spectrometer (10 citations) 400-MR 400 MHz spectrometer (8 citations) Unity-Plus-400 spectrometer (7 citations) 400 or 500 MHz spectrometers (4 citations) Avance 400-MHz spectrometer (4 citations)

26 protocols using 400 spectrometer

NMR Spectroscopic Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proton nuclear magnetic resonance (¹H NMR) spectra were recorded on a Varian 400 spectrometer and proton-decoupled carbon-13 NMR (¹³C {¹H} NMR) spectra were recorded on a Varian 500 or Varian 400 spectrometer at 25°C. Spectra were reported in parts per million (ppm) downfield of tetramethylsilane and are referenced to the residual resonances of the protium NMR solvent [CD₃OD: 3.31 (methanol)] and carbon NMR solvent [CD₃OD: 49.00 (methanol)]. MestReNova (v12.0.3) was used for all chemical NMR analysis. Data are reported as chemical shift, multiplicity (br, broad; s, singlet; d, doublet; t, triplet; q, quartet; quin, quintet; sept, septet; m, multiplet), coupling constants in Hertz (Hz) and integration. High-resolution mass spectrometric data were obtained on a Thermo Exploris 240 Orbitrap mass spectrometer by the Stanford University Mass Spectrometry (SUMS) core facility.

Ahn G., Riley N.M., Kamber R.A., Wisnovsky S., Moncayo von Hase S., Bassik M.C., Banik S.M, & Bertozzi C.R. (2023). Elucidating the cellular determinants of targeted membrane protein degradation by lysosome-targeting chimeras. Science (New York, N.Y.), 382(6668), eadf6249.

+ Open protocol

+ Expand

Spectroscopic Characterization of Synthesized Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

¹H and ¹³C NMR spectra were recorded on a Varian 400 spectrometer (at 500 MHz or at 125 MHz, respectively) or on a Varian 400 spectrometer (at 400 MHz or at 100 MHz, respectively) in CDCl₃ or DMSO-d₆. Structures were also confirmed with HMBC techniques. IR spectra were recorded on a Bruker Vertex 70 FT/IR spectrometer. Melting points were determined in open capillarity tubes with a Buchi B-535 apparatus and were uncorrected. Mass spectra were obtained with an Agilent 1100 spectrometer under chemical ionization conditions. Column chromatography was performed using Macherey-Nagel Silica 60, 0.04–0.063 mm silica gel.

Frasinyuk M.S., Mrug G.P., Bondarenko S.P., Sviripa V.M., Zhang W., Cai X., Fiandalo M.V., Mohler J.L., Liu C, & Watt D.S. (2015). Application of Mannich Bases to the Synthesis of Hydroxymethylated Isoflavonoids As Potential Antineoplastic Agents. Organic & biomolecular chemistry, 13(46), 11292-11301.

+ Open protocol

+ Expand

Spectroscopic Characterization of Compound 12

Check if the same lab product or an alternative is used in the 5 most similar protocols

General: IR spectra were recorded on a diamond anvil spectrophotometer. UV spectra were recorded using a Perkin‐Elmer Lambda 25 UV‐VIS spectrometer with EtOH as the solvent. ¹H and ¹³C NMR spectra were recorded at 600 MHz and 150 MHz, respectively, using a Varian 400 spectrometer. Chemical shifts, δ are given in ppm relative to the residual solvent and coupling constants, J are given in Hz. Low resolution and high resolution mass spectra were obtained at the University of Wales, Swansea using electron impact ionisation and chemical ionisation. Melting points were determined on a Kofler hot‐stage microscope. Compound 12 has been reported previously.1

Plater M.J, & Harrison W.T. (2019). An Organic Zeolite With 10 Å Diameter Pores Assembles From a Soluble and Flexible Building Block by Non‐Covalent Interactions. ChemistryOpen, 8(4), 457-463.

+ Open protocol

+ Expand

Characterization of New Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The melting points of the new compounds were measured in a microfusion melting point apparatus (X-4) (Taike, Beijing, China) and uncorrected. ¹H-NMR and ¹³C-NMR spectra were recorded on Varian 400 spectrometer at 400 MHz and 101 MHz using tetramethylsilane as internal standard (solvent CDCl₃ or DMSO-d₆). HR-MS date were detected on an FTICR-MS Varian 7.0T FTICR-MS equipment (Agilent, Lexington, MA, USA). Crystal structure was recorded on a Bruker SMART 1000CCD diffraction meter.

Gao W., Li X., Ren D., Sun S., Huo J., Wang Y., Chen L, & Zhang J. (2019). Design and Synthesis of N-phenyl Phthalimides as Potent Protoporphyrinogen Oxidase Inhibitors. Molecules, 24(23), 4363.

+ Open protocol

+ Expand

Analytical Methods and Telmisartan Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 1

Analytical Methods. NMR spectra were recorded using a Varian-400 spectrometer for ¹H (400 MHz). Chemical shifts (δ) are given in ppm downfield from tetramethylsilane, as internal standard, and coupling constants (J-values) are in hertz (Hz). Purifications by flash chromatography were performed. Liquid chromatography/mass spectrometry (LC/MS) analyses were conducted using Shimadzu LC-20AD pumps and a SPD-20A UV-vis detector. High-resolution mass spectra (HMRS) were recorded on a QSTAR Elite mass spectrometer.

Telmisartan Extraction. Telmisartan tablets were triturated, suspended in methanol and stirred for about 20 mins. Filtered off the solid, the methanol solution was concentrated, and the residue was purified by chromatography to afford white solid in 90% yield. ¹H NMR (CDCl₃, 400 MHz) δ 8.38 (m, 1H), 8.02 (dd, 1H, J=1.2, 1.2 Hz), 7.39 (m, 8H), 7.17 (s, 1H), 7.15 (s, 1H), 7.04 (s, 1H), 6.95 (s, 1H), 5.40 (s, 2H), 3.74 (s, 3H), 3.13 (t, 2H, J=7.6, 8.0 Hz), 2.69 (s, 3H), 1.99 (m, 2H), 1.15 (t, 3H, J=7.6, 7.2 Hz).

US11311526B2. Radiation mitigator and method of use thereof (2022-04-26). Georgetown University [US], The Board of Regents of the University of Oklahoma [US]. Inventors: Milton L. Brown [US], Yali Kong [US], Courtney Houchen [US], Sripathi M. Sureban [US], Parthasarathy Chandrakesan [US].

+ Open protocol

+ Expand

Spectroscopic Characterization of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

IR spectra were recorded on an ATI Mattson Fourier transform infrared (FTIR) spectrometer using KBr discs. Ultraviolet (UV) spectra were recorded using a PerkinElmer Lambda 25 UV/Vis spectrometer with EtOH as the solvent. ¹H and ¹³C nuclear magnetic resonance (NMR) spectra were recorded at 400 and 100.5 MHz, respectively, using a Varian 400 spectrometer. Chemical shifts, δ, are given in ppm and measured by comparison with the residual solvent. Coupling constants, J, are given in Hz. Copies of the NMR spectra are provided in the Supporting Information. High‐resolution mass spectra were obtained at the University of Wales, Swansea, using an Atmospheric Solids Analysis Probe (ASAP) (Positive mode) Instrument: Xevo G2‐S ASAP. Melting points were determined on a Kofler hot stage microscope. The starting materials 3–5 were purchased from Sigma‐Aldrich and compound 6 from Fluorochem. The purity of new compounds was ascertained by Thin Layer Chromatography (TLC) on alumina‐backed TLC plates.

Plater M.J, & Harrison W.T. (2021). A Potential Iterative Approach to 1,4‐Dihydro‐N‐Heteroacene Arrays. ChemistryOpen, 11(3), e202100150.

+ Open protocol

+ Expand

Characterization of Cisplatin Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cisplatin was obtained from Sigma Aldrich (St. Louis, MO 63146 USA). ¹H and ¹³C NMR spectra were recorded on a Varian 500 spectrometer (at 500 MHz or at 125 MHz, respectively) or on a Varian 400 spectrometer (at 400 MHz or at 100 MHz, respectively) in deuteriochloroform (CDCl₃) or deuterated dimethylsulfoxide (DMSO-d₆). IR spectra were recorded on a Bruker Vertex 70 FT/IR spectrometer. Melting points were determined in open capillarity tubes with a Buchi B-535 apparatus and were uncorrected. Mass spectra were obtained with an Agilent 1100 spectrometer under chemical ionization conditions.

Popova A.V., Frasinyuk M.S., Bondarenko S.P., Zhang W., Xie Y., Martin Z.M., Cai X., Fiandalo M.V., Mohler J.L., Liu C., Watt D.S, & Sviripa V.M. (2018). Efficient Synthesis of Aurone Mannich Bases and Evaluation of their Antineoplastic Activity in PC-3 Prostate Cancer Cells. Chemicke zvesti, 72(10), 2443-2456.

+ Open protocol

+ Expand

NMR, Fluorescence, and HPLC Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

NMR spectra were recorded on a Varian 400 spectrometer or on a Varian 500 spectrometer using DMSO-d₆ as the solvent. ¹H-NMR titrations were performed on a Varian 500 spectrometer. The resonance multiplicity is described as s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), dd (doublet of doublets), or br (broad signal).
Fluorescence measurements were performed on a Synergy H1 hybrid reader (Biotek) spectrofluorometer and on a Varian-Cary Eclypse spectrofluorometer with a thermostat at 25 °C.
TEM images were recorded with a Camera Olympus QUEMESA and software RADIUS (EMSIS) on a TEM images Philips EM208 at 100 KV using a 200-mesh copper grid with carbon film.
Rebinding tests were performed on a 1290 UHPLC system (Agilent, Germany), consisting of a degasser, a quaternary pump, a thermostated column compartment, and a diode array detector (DAD) operating at 324 nm and 273 nm, and on a HPLC Thermo scientific Vanquish Dionex Softron consisting of a degasser, a quaternary pump, a thermostated column compartment, and a DAD operating at 325 nm and 270 nm.

Gutiérrez-Ortiz A.L., Vida V., Peterka M., Tušar J., Berti F., Navarini L, & Forzato C. (2022). Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts. Sensors (Basel, Switzerland), 22(24), 9874.

+ Open protocol

+ Expand

Synthetic Methods for Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents and solvents were obtained from commercial suppliers and used as received unless otherwise indicated. Dry solvents were obtained according to the standard procedures. All reactions were performed under inert atmosphere (N₂) unless otherwise noted. Analytical silica gel-coated TLC plates (silica gel 60 F254) were purchased from EM Science and were visualized with UV light or by treatment with either phosphomolybdic acid (PMA) or ninhydrin. Flash chromatography was carried out on Baker Silica Gel 40 µM. 1H NMR and ¹³C spectra were routinely recorded with a Varian 400 spectrometer operating at 400 and 100 MHz, respectively. The NMR solvent used was either CDCl₃ or CD₃OD as indicated. TMS was used as an internal standard. NMR and rotation of free bases were recorded. Salts of free bases were used for biological characterization. Elemental analyses were performed by Atlantic Microlab Inc. and were within ± 0.4% of the theoretical value. Optical rotations were recorded on a Perkin-Elmer 241 polarimeter.

Santra S., Sharma H., Vedachalam S., Antonio T., Reith M, & Dutta A. (2014). Development of potent dopamine-norepinephrine uptake inhibitors (DNRIs) based on a (2S,4R,5R)-2-benzhydryl-5-((4-methoxybenzyl)amino)tetrahydro-2H-pyran-4-ol molecular template. Bioorganic & medicinal chemistry, 23(4), 821-828.

+ Open protocol

+ Expand

Copolymer Characterization via NMR and GPC

Check if the same lab product or an alternative is used in the 5 most similar protocols

¹H-NMR spectra (in CDCl₃) was performed at room temperature with a Varian 400 spectrometer (Varian, USA) at 400 MHz to characterize chemical composition of the copolymers. The samples were dissolved in CDCl₃, and the chemical shifts were given in ppm using tetramethylsilane (TMS) as an internal reference. GPC (Agilent 110 HPLC, USA) was also used to determine the macromolecular weight and macromolecular weight distribution of the prepared copolymers. The samples were dissolved in freshly distilled tetrahydrofuran (THF) at a concentration of 1 mg/ml. THF was eluted at a rate of 1.0 ml/min. The molecular weights of samples were calibrated with polystyrene (PS) as standard.

Shi K., Wang Y.L., Qu Y., Liao J.F., Chu B.Y., Zhang H.P., Luo F, & Qian Z.Y. (2016). Synthesis, characterization, and application of reversible PDLLA-PEG-PDLLA copolymer thermogels in vitro and in vivo. Scientific Reports, 6, 19077.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!