Stat high sensitive troponin 1 assay

Standard biochemical parameters were assessed in the laboratory at the Faculty Hospital in Pilsen. The estimated glomerular filtration rate (eGFR) was calculated using the Modification of Diet in Renal Disease (MDRD) formula. Albuminuria is expressed as a urinary albumin-to-creatinine ratio (ACR). High-sensitivity cardiac troponin I (hsTnI) was measured using the Architect i2000 platform with STAT High Sensitive Troponin-I assay (Abbott Diagnostics, USA) (limit of quantification 4–10 ng/l, 10 % coefficient of variation 4.7 ng/l, 99th percentile 26.2 ng/l, percentage of measurable values in healthy population >80 %). Plasma levels of osteoprotegerin were measured using multiplex immunoanalyses based on xMAP® technology, commercially available kits MILLIPLEX MAP Human Bone Panel 1A magnetic (Merck-Millipore Corporation, USA) and the MAGPIX instrument (Luminex Corporation, USA). xMAP® technology and the MAGPIX system uses color-coded magnetic microspheres coated by antibodies to perform quantitative sandwich immunoanalysis of proteins in a variety of sample matrices.

N-terminal pro-brain natriuretic peptide (NT-proBNP) was determined using original analytical kits from Roche (Prague division, Prague, Czech Republic) on a Cobas 8000 analyzer. High-sensitivity cardiac troponin I (hsTnI) was measured using the Architect i2000 platform with the STAT High Sensitive Troponin-I assay (Abbott Diagnostics, Abbot Park, IL, USA). Growth/Differentiation Factor-15 (GDF-15; RayBiotech, Norcross, GA, USA) and galectin-3 (MyBiosource, San Diego, CA, USA) concentrations were determined using ELISA kits on a Nexgen ELISA four reader (Adaltis, Rome, Italy). Suppression of tumorigenicity2 (sST2) was measured using a Critical Diagnostics Presage^® ST2 Assay kit (Critical Diagnostics, San Diego, CA, USA). Routine biochemical parameters including renal functions were also measured in all subjects and did not change over time.