All in vivo experiments were performed in accordance with the guidelines provided by the Animal Ethics Committee of Renji Hospital. Five‐week‐old male BALB/c nude mice (Shanghai Laboratory Animal Center, Shanghai, China) were injected subcutaneously with LDHB‐KO HCT116/DLD1 cells (5 × 106 cells) stably expressing EGFP‐3Flag‐LDHBWT/K329R/K329Q. All mice were killed at 21 days after injection, and tumour tissues were collected and weighed. Five‐week‐old male BALB/c nude mice (Shanghai Laboratory Animal Center) received subcutaneous injections of 5 × 106 LDHB‐KO HCT116 cells that were stably transfected with EGFP‐3Flag‐LDHBWT or EGFP‐3Flag‐LDHBK329Q in 100 μL of saline at the right dorsal flank. Fourteen days after tumour implantation, the mice were randomly assigned to a treatment group receiving GW5074 (5 mg·kg−1) or a control group receiving saline three times per week intraperitoneally for 21 days. At the end of the experiment, animals were sacrificed, and tumour tissues were collected for weighing and immunohistochemical analysis.
Balb c nude mice
Manufactured by Shanghai Laboratory Animal Center
Sourced in China
BALB/c nude mice are an inbred strain of mice that are athymic, meaning they lack a functional thymus gland and are therefore immunodeficient. This strain is widely used in biomedical research, particularly in the study of human cancer and the evaluation of therapies.
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Lab products found in correlation
Minimr 60, by Niumag (3 mentions)
Balb c mice, by Shanghai Laboratory Animal Center (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Matrigel solution, by BD (2 mentions)
Sh nc, by GenePharma (2 mentions)
Matrigel, by Corning (2 mentions)
Pentobarbital sodium, by Merck Group (1 mentions)
D12492, by Research Diets (1 mentions)
Ivis imaging system, by Bruker (1 mentions)
Goat anti mouse igg, by Beyotime (1 mentions)
Mouse anti β actin, by Beyotime (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Ivis spectrum, by PerkinElmer (1 mentions)
Matrigel, by BD (1 mentions)
Sh vps9d1 as1, by GenePharma (1 mentions)
C57bl 6 mice, by Shanghai Laboratory Animal Center (1 mentions)
Monoethanolamine, by Sinopharm (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
211 protocols using balb c nude mice
1
Investigating LDHB's Role in Tumor Growth
2
Mammary Gland Tumor Xenograft Model in Nude Mice
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A total of 30 specific pathogen-free (SPF) female nude Balb/c mice (6–8 weeks old) were purchased from Shanghai Laboratory Animal Center (Shanghai, China). All rats were housed in a temperature-controlled facility at 23±1°C with a relative humidity of 50±5% and were exposed to a 12-h light/dark cycle. The mammary glands of mice were subcutaneously implanted with βig-h3-overexpressed, si-Rβig-h3-treated or si-Rvector-transfected MCF-7 cells (1×107) and were subsequently divided into three groups (n=10 in each group). Mice were observed for 40 days following tumor inoculation. Tumor diameters were recorded every 2 days and tumor volume was calculated using the following formula: 0.52 × smallest diameter2 × largest diameter (17 (link)). Experimental mice were euthanized when the tumor diameter reached 10 mm. The present study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of China (18 (link)). The present study was also approved by the Ethics Committee of Pingdu People's Hospital (Qingdao, China). All surgery was performed following intraperitoneal injection of sodium pentobarbital (40 mg/kg, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) to induce anesthesia and all efforts were made to minimize suffering.
3
Xenograft Model of Tumor Growth in Nude Mice
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Nude Balb/c mice (6–8 weeks old, weighing 18–22 g) were provided by Shanghai Laboratory Animal Center. CAL-27 and shCAL-27 cells (5 × 106 cells/mice) were suspended and transplanted subcutaneously into the armpit of nude mice. Tumor length and width were measured every three days, calculating tumor volume (V = 0.5 × L × W [2 (link)], V: volume, L: length, and W: width) [19 (link)]. After 29 days, all mice were killed, and xenograft tumors were removed for examination and fixed with 4% paraformaldehyde. Protein expression level of KIF22 was detected by western blot and immunohistochemical staining in xenograft tumors.
4
Circular RNA Regulation in A549 Cells
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Twelve male nude BALB/c mice (20–22 g, 4 weeks) were purchased from Shanghai Laboratory Animal Center (SLAC, Shanghai, China). A549 cells transfected with sh-circ_0001421 or sh-NC were injected into the nude mice. Tumor volume was measured once a week. Five weeks later, all the mice were euthanatized with an overdose of ether, and tumor weight was measured. Western blot and qRT-PCR were performed to examine the expression of CDCA3 and circ_0001421 or miR-4677-3p, respectively.
5
Tumor Growth with Fibroblast Co-Injection
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Six-week-old BALB/c nude mice were purchased from Shanghai Laboratory Animal Center. The animals were operated according to the protocol approved by the Institutional Animal Care and Use Committee of the Shanghai Jiao Tong University School of Medicine. Tumor xenografts were generated by subcutaneously co-injecting A375 cells with human non-activated fibroblasts (NAFs) or CAFs (cancer-associated fibroblasts) into the armpits bilaterally, the ratio of A375 to fibroblasts was 1:3, and the total cell number in each injection was 4 × 106. NAFs were depleted of IDH1 or infected by empty viruses. The tumor volumes in the three groups were determined weekly using digital caliper measurements and the following formula: tumor volume (mm3) = ½ × longest diameter × shortest diameter2. After 9 weeks, the mice were sacrificed and the tumors were excised.
6
BALB/c Nude Mice Xenograft Model
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All of the animal care and experimental procedures were performed in accordance with the Laboratory Animal Care Guidelines and approved by the Animal Care and Use Committee of Zhongshan Hospital, Fudan University.
Four-week-old male BALB/c nude mice were purchased from the Shanghai Experimental Animal Center and subcutaneously injected with 1 × 107 LM3 cells transfected with negative control (SCR) or GTSE1-SH. The tumor dimensions were measured with vernier calipers every 1 week. Mice were sacrificed 5 weeks after the subcutaneous injection, following the xenografts weights were immediately measured.
Four-week-old male BALB/c nude mice were purchased from the Shanghai Experimental Animal Center and subcutaneously injected with 1 × 107 LM3 cells transfected with negative control (SCR) or GTSE1-SH. The tumor dimensions were measured with vernier calipers every 1 week. Mice were sacrificed 5 weeks after the subcutaneous injection, following the xenografts weights were immediately measured.
7
Xenograft Mouse Model of CircRNA
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For in vivo analysis, BALB / c nude mice (5/group) were purchased from the Shanghai Experimental Animal Center. We suspended SK-HEP-1 cells (1 × 10 6 cells/mL) with stable hsa_circ_0056836 expression or miR-766-3p in 100 μL PBS, BALB/c nude mice were subcutaneously injected with the SK-HEP-1 cells containing with different miRNAs. All mice were sacrificed by carbon dioxide euthanasia with tumors being dissected and collected 5 weeks later. The animal work took place in the First Affiliated Hospital of Zhengzhou University. The study was performed in strict accordance with the guidelines adhered to the Guide for the Care and Use of Laboratory Animals and the protocol was approved by the Committee on the Ethics of Animal Experiments at the First Affiliated Hospital of Zhengzhou University.
8
Xenograft Model for EOC in Nude Mice
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Five-week-old female BALB/c nude mice were purchased from Shanghai Laboratory Animal Center and bred in the Laboratory Animal Center of Fudan University Shanghai Cancer Center under speci cpathogen-free conditions. Mice were maintained under a 12 h light/12 h dark cycle with free access to water and standard mouse diet. EOC cell suspensions (5 × 10 5 cells/100 µL of DMEM) were mixed with 100 µL of Matrigel solution (BD) and injected into the back anks of the nude mouse. The tumor size was measured using a Vernier caliper once a week, and the tumor volume was calculated using the following formula: V = (D × d )/2 mm, where D is the longest diameter and d is the shortest diameter of the tumor. The mice were sacri ced 5 weeks after implantation, and the tumors were weighed and photographed.
9
Xenograft Tumor Growth in BALB/c Mice
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Female 6-week-old BALB/c nude mice were obtained from the Shanghai Laboratory Animal Center (Shanghai, P.R. China). All mice were randomly divided into two groups (seven mice/group) and handled according to the procedure approved by the Institutional Animal Care and Use Committee of the Huaihe Hospital of Henan University. Transfected cells (4 × 103) were suspended in 100 μl of PBS and then injected subcutaneously into the left flank of each mouse. Tumor size was measured every 6 days. After 30 days, all mice were sacrificed for thorough removal and weighing of tumors. Tumor volume was calculated according to the following formula: volume = (length × width2)/2.
10
Subcutaneous Xenograft Tumor Model
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Approximately 1 × 106 cells were injected subcutaneously into the right neck of male BALB/C nude mice (age, 4–6 weeks; weight, 18–22 g, five mice per group), purchased from SLACCAS (Shanghai Laboratory Animal Center, CAS, Shanghai, China). The length and width of tumor xenografts were measured weekly by vernier calipers, and the tumor volume was calculated using the following formula: volume (cubic millimeters) = 0.5 × width2 × length. Six weeks after injection, the mice were killed by cervical dislocation. All experimental procedures were taken place at the animal center of The Second Affiliated Hospital of Zhejiang University School of Medicine and approved by the Animal Care and Use Committee of The Second Affiliated Hospital of Zhejiang University School of Medicine, and animal experiments were performed following the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
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