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Anti oga

Manufactured by Novus Biologicals
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Anti-OGA is a lab equipment product manufactured by Novus Biologicals. It is used for the detection and quantification of O-GlcNAcase (OGA) activity in biological samples.

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Lab products found in correlation

Anti o glcnac rl2, by Abcam (2 mentions) Anti pkm2, by Cell Signaling Technology (2 mentions) Anti actin, by Merck Group (2 mentions) Anti acetyl lysine ack, by Cell Signaling Technology (2 mentions) Anti hn, by Takara Bio (2 mentions) Anti ogt, by Cell Signaling Technology (2 mentions) Anti flag, by Merck Group (2 mentions)

Close spelling variants of this product

Anti-OGA antibody (NBP2-32233) (2 citations)

2 protocols using anti oga

1

Profiling Protein Acetylation and O-GlcNAcylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells and tumors were lysed in a 1% Nonidet P-40, 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA buffer with protease, protein phosphatase, and O-GlcNAcase inhibitors. Proteins were immunoprecipitated by incubating whole cell lysates/tumor homogenates with the indicated antibodies for 2 hours and precipitating with Protein A/G agarose beads. Whole cell lysates/tumor homogenates and immunoprecipitation samples were subjected to 8% SDS-PAGE gel electrophoresis and transferred to PVDF membranes. Membranes were incubated with the indicated primary antibodies overnight at 4°C. Western blots were visualized using HRP-conjugated secondary antibodies and ECL. The following antibodies were used: anti-actin (Sigma), anti-acetyl-lysine (AcK, Cell Signaling), anti-Flag (Sigma), anti-HN (Clontech), anti-OGT (Cell Signaling), anti-OGA (Novus), anti-O-GlcNAc (RL2, Abcam), and anti-PKM2 (Cell Signaling).
Singh J.P., Qian K., Lee J.S., Zhou J., Han X., Zhang B., Ong Q., Ni W., Jiang M., Ruan H.B., Li M.D., Zhang K., Ding Z., Lee P., Singh K., Wu J., Herzog R.I., Kaech S., Wendel H.G., Yates JR I.I.I., Han W., Sherwin R.S., Nie Y, & Yang X. (2019). O-GlcNAcase targets pyruvate kinase M2 to regulate tumor growth. Oncogene, 39(3), 560-573.
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2

Profiling Protein Acetylation and O-GlcNAcylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells and tumors were lysed in a 1% Nonidet P-40, 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA buffer with protease, protein phosphatase, and O-GlcNAcase inhibitors. Proteins were immunoprecipitated by incubating whole cell lysates/tumor homogenates with the indicated antibodies for 2 hours and precipitating with Protein A/G agarose beads. Whole cell lysates/tumor homogenates and immunoprecipitation samples were subjected to 8% SDS-PAGE gel electrophoresis and transferred to PVDF membranes. Membranes were incubated with the indicated primary antibodies overnight at 4°C. Western blots were visualized using HRP-conjugated secondary antibodies and ECL. The following antibodies were used: anti-actin (Sigma), anti-acetyl-lysine (AcK, Cell Signaling), anti-Flag (Sigma), anti-HN (Clontech), anti-OGT (Cell Signaling), anti-OGA (Novus), anti-O-GlcNAc (RL2, Abcam), and anti-PKM2 (Cell Signaling).
Singh J.P., Qian K., Lee J.S., Zhou J., Han X., Zhang B., Ong Q., Ni W., Jiang M., Ruan H.B., Li M.D., Zhang K., Ding Z., Lee P., Singh K., Wu J., Herzog R.I., Kaech S., Wendel H.G., Yates JR I.I.I., Han W., Sherwin R.S., Nie Y, & Yang X. (2019). O-GlcNAcase targets pyruvate kinase M2 to regulate tumor growth. Oncogene, 39(3), 560-573.
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