Nisoldipine

Manufactured by Merck Group

Sourced in United States

Nisoldipine is a laboratory reagent used in the analysis and testing of various chemical and biological samples. It is a calcium channel blocker that can be used in various analytical techniques, such as chromatography and spectroscopy, to help identify and quantify specific compounds. Nisoldipine is a versatile and widely used lab equipment product, but its detailed description and intended use should be provided by subject matter experts to ensure accuracy and objectivity.

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Lab products found in correlation

8 protocols using nisoldipine

Patch Clamp Analysis of Cardiac Ion Currents

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To assess the effects of the compounds on AP and ion currents, patch clamp experiments were performed using Axopatch 200B patch clamp amplifier (Molecular Devices) and pCLAMP software (Axon Instruments).
APs were recorded in current clamp with 2–3 ms 0.4 mA square pulses at 0.5, 1 and 2 Hz. Membrane currents were recorded using the whole cell configuration. For studies of I_K (I_Kr + I_Ks), and I_K1 cardiomyocytes were superfused with normal Tyrode solution. To measure I_Kr, I_Ca,L and I_Ks were inhibited by 1 µM nisoldipine (Sigma) and 30 µM chromanol 293B (Sigma). I-V relationship for I_K and I_Kr tail currents were determined by applying 1.5 s depolarizing voltage pulses from holding potential of −40 mV to test potentials ranging from −30 to + 60 mV. Tail current was measured upon repolarization to −40 mV.
I_K1 was elicited from a holding potential of −20 mV by voltage steps from −120 mV to + 50 mV. Steady-state I_K1 amplitudes were estimated at the end of the 500 ms pulse. All experiments were carried out at room temperature.

Kreifels P., Bodi I., Hornyik T., Franke G., Perez-Feliz S., Lewetag R., Moss R., Castiglione A., Ziupa D., Zehender M., Brunner M., Bode C, & Odening K.E. (2022). Oxytocin exerts harmful cardiac repolarization prolonging effects in drug-induced LQTS. International Journal of Cardiology. Heart & Vasculature, 40, 101001.

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Patch Clamp Analysis of Late Na+ Current

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Myocytes were placed in a bath mounted on top of an inverted microscope (Olympus IX71; Olympus, Canada), and rod‐shaped quiescent myocytes were selected for the study. Myocytes were superfused with modified Tyrode's solution at 35 to 36 °C. Pipettes with a resistance of 1.5–2.5 MOhm filled with Cs⁺ solution (in mmol/L: 25 CsCl, 5 NaCl, 110 CsOH, 110 aspartic acid, 5 MgATP, 5 EGTA, and 10 HEPES) were zeroed in the solution, then used to form a tight seal, and after that, the membrane under the pipette was ruptured using the zap function of the amplifier and gentle suction. Current and membrane potential was measured using a Multiclamp 700B amplifier (Molecular Devices, USA) in the voltage‐clamp mode. The measured signal was digitized at 10 kHz by 16‐bit analog‐digital board DigiData 1440A (Molecular Devices) under the control of pClamp 10 software (Molecular Devices, USA) and stored for offline analysis. Late Na⁺ current was measured in nominally K⁺/Ca²⁺‐free modified Tyrode's solution as superfusate with 1 μmoL/L nisoldipine (Sigma Aldrich, Canada). The current was elicited by depolarizations from −120 (pre‐pulse) to −40 mV. tetrodotoxin in citrate buffer (5 μmoL/L tetrodotoxin; Abcam, USA) was used to record background and isolate I_Na‐L.¹⁴

Zhabyeyev P., McLean B., Bassiouni W., Valencia R., Paul M., Darwesh A.M., Seubert J.M., Hazra S, & Oudit G.Y. (2023). Loss of PI3Kα Mediates Protection From Myocardial Ischemia–Reperfusion Injury Linked to Preserved Mitochondrial Function. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 12(12), e022352.

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Embryonic Development Modulation

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Blebbistatin (5 uM), nisoldipine (10 uM), cyclosporine A (CsA, 10 μg/ml), FK506 (1 μg/ml), and BayK8644 (20 uM) (all from Sigma-Aldrich, St. Louis, MO) were dissolved in DMSO and diluted to final working concentration in embryo media without antibiotics. Embryos (24 hpf) were chemically and manually dechorionated using pronase. Drug solutions were applied to embryos at 24 hpf and re-applied every 6 hours during the period of drug treatment. Media containing an equivalent volume of DMSO was used as control. Embryos were incubated at 28.5°C in the dark to prevent light degradation of drugs.

Andersen N.D., Ramachandran K.V., Bao M.M., Kirby M.L., Pitt G.S, & Hutson M.R. (2014). Calcium Signaling Regulates Ventricular Hypertrophy During Development Independent of Contraction or Blood Flow. Journal of molecular and cellular cardiology, 80, 1-9.

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Evaluating Ion Channel Modulators

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Nisoldipine, NiCl₂, 4‐aminopyridine (4‐AP), and ATX‐II were purchased from Sigma‐Aldrich Co (St. Louis, MO). Stock solutions for the drugs were prepared according to the vendor's instructions and then diluted for studies as needed.

Stroud D.M., Yang T., Bersell K., Kryshtal D.O., Nagao S., Shaffer C., Short L., Hall L., Atack T.C., Zhang W., Knollmann B.C., Baudenbacher F, & Roden D.M. (2016). Contrasting Nav1.8 Activity in Scn10a −/− Ventricular Myocytes and the Intact Heart. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(11), e002946.

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Bladder Contractility Pharmacological Modulators

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Tetrodotoxin, phentolamine, iberiotoxin, NS-1619, Y-27632, apamin, NS-309, and ryanodine were purchased from Abcam (Cambridge, MA, USA). Atropine, propranolol, suramin, nisoldipine, and SKF-96365 were purchased from Sigma-Aldrich (St. Louis, MO USA). Bisindolylmaleimide 1 and H₂O₂ were obtained from Cayman Chemical (Ann Arbor, MI, USA) and Fisher Scientific (Pittsburg, PA, USA), respectively.
NS-1619, nisoldipine, NS 309, and SKF-96365 were dissolved in dimethylsulphoxide (DMSO). ryanodine was dissolved in 100% ethanol. All other drugs were prepared with water. Final ethanol and DMSO concentrations in the bath solution did not exceed 0.1% and were shown not to affect spontaneous contractions or bladder function in previous reports [35 (link)–37 (link)].

Wang M., Xing N., Wu L., Huang W.C., Xu Z, & Liu G. (2018). Regulation of Spontaneous Contractions in Intact Rat Bladder Strips and the Effects of Hydrogen Peroxide. BioMed Research International, 2018, 2925985.

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Screening Library of FDA-Approved Drugs

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The Screen-Well FDA-approved drug library was purchased from Enzo Life Sciences (catalogue no. BML-2841; Farmingdale, NY, USA). The 640 compounds in this library were provided at 2 mg/mL in dimethyl sulfoxide (DMSO). Flubendazole (FLB), mebendazole (MEB), benomyl (BEN), nisoldipine (NIS), nifedipine (NIF), felodipine (FEL), niguldipine (NIG), amphotericin B (AMB), itraconazole (ITZ), voriconazole (VRZ) and 5-flucytosine (5FC) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fluconazole (FLC) was obtained from Cayman Chemical (Ann Arbor, MI, USA). Stock solutions of amphotericin B, itraconazole and voriconazole were prepared at 1.6 mg/mL in water; 5-flucytosine was made at 6.4 mg/mL in water; fluconazole was made at 12.8 mg/mL in water and all remaining drugs were dissolved in DMSO at 2 mg/mL. All compounds were stored at −80 °C.

Truong M., Monahan L.G., Carter D.A, & Charles I.G. (2018). Repurposing drugs to fast-track therapeutic agents for the treatment of cryptococcosis. PeerJ, 6, e4761.

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Characterization of Drug Metabolism Assays

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Uridine 59-diphosphoglucuronic acid trisodium salt (UDPGA), NADPH, alamethicin from Trichoderma viride, adenosine 3-phosphate 5-phosphosulfate, diclofenac sodium salt, midazolam hydrochloride, felodipine, S-(+)-mephenytoin, raloxifene hydrochloride, lovastatin, nisoldipine, nifedipine, methadone, quinidine, testosterone, benzydamine hydrochloride, cisapride, cyclosporin, terbutaline, sildenafil, verapamil, trazodone, atorvastatin, simvastatin, buspirone, rifabutin, saquinavir, terfenadine, zolpidem, repaglinide, indinavir, alprazolam, carbazeran, enalapril, ramipril , and dabigatran etexilate were purchased from Sigma-Aldrich (St. Louis, MO). NADPH-regenerating system containing NADP + , glucose-6phosphate, and glucose-6-phosphate dehydrogenase was purchased from Corning (Wiesbaden, Germany). Organic solvents were of LC-mass spectrometry (MS) or higher quality grade and were acquired from VWR International (Radnor, PA) or Fisher Scientific UK Ltd (Loughborough, UK). Purified water was obtained from Milli-Q Integral 5 Water Purification System (Merck KGaA, Darmstadt, Germany). Phosphate buffer, HQM Hepatocyte/Enterocyte Incubation Medium, and Co-Factor N for MetMax were purchased from In Vitro ADMET Laboratories (Columbia, MD).

Davies M., Peramuhendige P., King L., Golding M., Kotian A., Penney M., Shah S, & Manevski N. (2020). Evaluation of In Vitro Models for Assessment of Human Intestinal Metabolism in Drug Discovery. Drug metabolism and disposition: the biological fate of chemicals, 48(11).

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Evaluation of Pharmacological Compounds

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The following drugs were used: BYL719 (BYL; ChemieTek, USA) as 10 µmol/l stock in DMSO, KB-R7943 mesylate (KB-R; Tocris Bioscience, USA) as 100 mmol/l stock in DMSO, Nisoldipine (Sigma Aldrich, USA) as 10 mmol/l stock in DMSO, ranolazine (Ran; Tocris Bioscience, USA) as 100 mmol/l stock in double-distilled water (ddH2O), and tetrodotoxin in citrate buffer (TTX, Abcam, USA) as 10 mmol/l stock in ddH2O. All stocks were stored at -20 °C.

Zhabyeyev P., McLean B., Chen X., Vanhaesebroeck B, & Oudit G.Y. (2019). Inhibition of PI3Kinase-α is pro-arrhythmic and associated with enhanced late Na⁺ current, contractility, and Ca²⁺ release in murine hearts. Journal of molecular and cellular cardiology, 132.

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