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Rab11

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RAB11 is a member of the Rab GTPase family, which are involved in the regulation of intracellular membrane trafficking. RAB11 is a small GTP-binding protein that plays a role in the recycling of endosomes and the transport of various cargo between the trans-Golgi network and the plasma membrane.

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Lab products found in correlation

Pggt1b, by Fortis Life Sciences (2 mentions) Rabggta, by Merck Group (2 mentions) Rabggta, by OriGene (2 mentions) Gfp tagged cdna of rab1a, by Addgene (2 mentions) Rab7a, by Addgene (2 mentions) Rab34, by Addgene (2 mentions) Rab35, by Addgene (2 mentions) Anti ha, by Fortrea (2 mentions) Pcdna3.1 myc his, by Thermo Fisher Scientific (1 mentions) Pcmv myc, by Takara Bio (1 mentions) Pcmv ha, by Takara Bio (1 mentions) Pegfp n1, by Takara Bio (1 mentions)

Close spelling variants of this product

GFP-rab11 WT MCherry-Rab11 GFP-Rab11 EGFP-RAB11 DsRed-Rab11

3 protocols using rab11

1

Validation of PTAR1 antibody specificity

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A rabbit polyclonal antibody against the peptide corresponding to amino acids 339 to 359 (CDGLNDSSKQGYSQETKRLKRT) of human PTAR1 primary sequence was custom made by YenZyme antibodies, LLC, CA 94080. The specificity of the antibody was validated by silencing PTAR1 in primary fibroblasts NHFs and HEK293T cell lines (Supplementary Fig. 1A). Antibodies were from Abcam (FNTA, FNTB), Abnova (PGGT1B), Bethyl (RabGGTB), Aviva Biosystems (RabGGTA), Sigma (anti-FLAG M2, anti-FLAG, β-actin), Santa Cruz Biotechnology (SKP1), and Covance (anti-HA). A polyclonal rabbit antibody to FBXL2 was generated using a peptide against FBXL2 as previously described27 (link). Human cDNA ORFs for PTAR1, FNTA, and RabGGTA were purchased from Origene. GFP-tagged cDNA of RAB1a, RAB7a, RAB11, RAB34, and RAB35 were obtained from Addgene. The variously tagged-genes and mutants were generated in pcDNA3 and pEGFPC1 vectors either by sub-cloning or site-directed mutagenesis methodologies, as described.
Kuchay S., Wang H., Marzio A., Jain K., Homer H., Fehrenbacher N., Philips M.R., Zheng N, & Pagano M. (2019). GGTase3 is a Newly Identified Geranylgeranyltransferase Targeting a Ubiquitin Ligase. Nature structural & molecular biology, 26(7), 628-636.
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2

Validation of PTAR1 antibody specificity

Cited 1 time
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A rabbit polyclonal antibody against the peptide corresponding to amino acids 339 to 359 (CDGLNDSSKQGYSQETKRLKRT) of human PTAR1 primary sequence was custom made by YenZyme antibodies, LLC, CA 94080. The specificity of the antibody was validated by silencing PTAR1 in primary fibroblasts NHFs and HEK293T cell lines (Supplementary Fig. 1A). Antibodies were from Abcam (FNTA, FNTB), Abnova (PGGT1B), Bethyl (RabGGTB), Aviva Biosystems (RabGGTA), Sigma (anti-FLAG M2, anti-FLAG, β-actin), Santa Cruz Biotechnology (SKP1), and Covance (anti-HA). A polyclonal rabbit antibody to FBXL2 was generated using a peptide against FBXL2 as previously described27 (link). Human cDNA ORFs for PTAR1, FNTA, and RabGGTA were purchased from Origene. GFP-tagged cDNA of RAB1a, RAB7a, RAB11, RAB34, and RAB35 were obtained from Addgene. The variously tagged-genes and mutants were generated in pcDNA3 and pEGFPC1 vectors either by sub-cloning or site-directed mutagenesis methodologies, as described.
Kuchay S., Wang H., Marzio A., Jain K., Homer H., Fehrenbacher N., Philips M.R., Zheng N, & Pagano M. (2019). GGTase3 is a Newly Identified Geranylgeranyltransferase Targeting a Ubiquitin Ligase. Nature structural & molecular biology, 26(7), 628-636.
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3

Construct Myc- and GFP-tagged Proteins

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Myc-tagged TMEM59L, TMEM59, and ATG3 were constructed by using the pCDNA3.1-myc/his (Invitrogen) construct as backbones. Myc-ATG5 was constructed by using the pCMV-myc (Clontech) construct as backbone; GFP-tagged TMEM59L and TMEM59 were constructed by using the pEGFP-N1 (Clontech) as backbones. HA-tagged LC3B and ATG16L1 were constructed by using the pCMV-HA (Clontech) vector as backbones. GFP-tagged Rab5 plasmid was kindly provided by Dr. Steve Caplan (University of Nebraska, Lincoln, NE); Rab7 (Addgene plasmid #12605) and Rab11 (Addgene plasmid #12674) were kindly provided by Dr. Richard Pagano (Mayo Clinic, Rochester, MN) and obtained from Addgene.
Pre-made adenoviruses encoding human TMEM59L cDNA fused with HA tag (Ad-59L-HA) and control adenovirus were purchased from Abmgood, and amplified in HEK293A cells and purified as described previously [17 (link)].
Zheng Q., Zheng X., Zhang L., Luo H., Qian L., Fu X., Liu Y., Gao Y., Niu M., Meng J., Zhang M., Bu G., Xu H, & Zhang Y.W. (2016). The Neuron-Specific Protein TMEM59L Mediates Oxidative Stress-Induced Cell Death. Molecular neurobiology, 54(6), 4189-4200.
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