B6.129 tlr2tm1kir j

Wild type BALB/cJ and C57BL/6J mice along with mutant knockout mice B6.129-Tlr2^tm1Kir/J, B6.129S1-Nod2^tm1Flv/J, and B6.129S1-Tlr5^tm1Flv/J were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Caspase1-/- on the C57BL/6 background were generously gifted from Dr. J. P. Ting (UNC-Chapel Hill) [18 (link)]. Nod2-/- mice on the BALB/c background were generously provided by Dr. Holly Rosenzweig (Oregon Health & Science University), bred at CSU, and Nod2-/- genotype was confirmed [19 (link)].
All animals were age matched (8–12 weeks), maintained in specific pathogen free conditions, individually tracked and monitored daily for clinical signs of stress or illness, including but not limited to changes in skin and hair, eyes and mucous membranes, respiratory system, circulatory system, central nervous system, salivation, diarrhea, or lethargy. Upon arrival, animals were housed socially (2–5) in commercially available, individually ventilated caging systems with a 12 h light/12 h dark cycle. Animals were provided ad libitum commercial irradiated rodent chow (Teklad Global) and tap water filtered via reverse osmosis in autoclaved water bottles; all bedding and enrichment materials was autoclaved prior to use and changed regularly.

All animal use protocols were approved by the MGH IACUC and carried out in accordance with national guidelines for the ethical use of animals in research. C57BL/6J (Jackson Laboratories strain [Bar Harbor, ME, USA] Number 000664), Tlr2^−/− (B6.129-tlr2^tm1Kir/J, Jackson Laboratories strain number 004650), Sting^−/− (C57BL/6J-Sting^gt/J, Jackson Laboratories strain number 017537), and cGAS^−/− (B6(C)-Cgas^{tm1d(EUCOMM)Hmgu}/J, Jackson Laboratories strain number 026554) mice were ordered from Jackson Laboratories. Mice were euthanized by carbon-dioxide inhalation, and femurs and tibias were harvested for bone marrow isolation. Bone marrow cells were incubated at 37°C with 5% carbon dioxide in BMDM media (DMEM [Gibco, Billings, Montana, USA] with 20% fetal bovine serum [Hyclone, Logan, Utah, USA] and 25 ng/mL recombinant mouse M-CSF [R and D Systems, Minneapolis, MN, USA]) on petri dishes. After 6 days, adherent cells were washed and harvested for use as bone marrow-derived macrophages.

C57Bl/6J (B6) and B6.SJL-Ptprc^aPepc^b/BoyJ (B6-CD45.1), and B6.129-Tlr2^tm1Kir/J (TLR2 KO) mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). TLR2 KO mice were bred in-house and were age-matched with vendor-purchased B6 mice. Female B6 and TLR2 KO mice were between 6 and 10 weeks old at the time of inoculation. All mice were housed in specific pathogen-free conditions at the University of Arizona in accordance with the Institutional Animal Care and Use Committee (IACUC).

Female, specific pathogen-free (SPF), 6–7-week-old C57BL/6J (H-2K^b and I-A^b), BALB/c (H-2Kd and I-A^d) mice were purchased from Japan SLC, Inc. (Shizuoka, Japan). Age- and gender-matched ovalbumin (OVA)-specific T cell receptor (TCR) transgenic mice, TLR2 knockout (K/O) mice (B6.129-Tlr2tm1Kir/J), and TLR4 K/O mice (C57BL/10ScNJ) in a C57BL/6J background were purchased from the Jackson Laboratory (Bar Harbor, ME). The animals were fed a sterile commercial mouse diet and provided with water ad libitum. Mice infected with Mtb H37Rv were housed under adequate conditions in a BL-3 biohazard animal barrier facility at the Avison Biomedical Research Center, Yonsei College of Medicine. The animal experiments complied with the Ethics Committee and Institutional Animal Care and Use Committee of Yonsei University Health System (Permission no.: 2016-019).

WT C57BL/6J (JAX:000664), CD45.1 (B6.SJL-Ptprc^a Pepc^b/BoyJ, JAX:02014), TLR2^–/– (B6.129-Tlr2^tm1Kir/J, JAX:004650), TLR4^–/– (B6.B10ScN-Tlr4^lps–del/JthJ, JAX:007227), Rag2^–/– (B6(Cg)-Rag2^tm.1Cgn/J, JAX:008449), and OTII (B6.Cg-Tg[TcraTcrb]425Cbn/J, JAX:004194) mice were purchased from The Jackson Laboratory. Tlr2^–/– Tlr4^–/– mice were generated by crossing TLR2^–/– and TLR4^–/– mice. Batf3^–/– (C.129S-Batf3^tm1Kmm/J, JAX:013756) mice were obtained via Miriam Merad (Precision Immunology Institute and Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA).

WT (C57BL/6J), TLR2^−/− (B6.129-Tlr2^tm1Kir/J), MYD88^−/− (B6.129P2(SJL)-Myd88^tm1.1Defr/J), and p47^phox−/− (B6N.129S2-Ncf1^tm1Shl/J) mice were purchased from The Jackson Laboratory. Female mice were 6–8 weeks old and were used for isolation of BMMs and mouse infections.
All protocols involving animals were approved by the Institutional Animal Care and Use Committee of Stony Brook University.