Protein lysates were extracted from tissues of injured spinal cord tissue 72 hrs post-injury. Protein concentration was determined using the BCA protein assay kit (CW0014S, CWBIO; Haimen, Jiangsu, China). Concentrations of 20–40 µg total protein were separated on polyacrylamide gels, transferred to polyvinylidene difluoride membranes and incubated overnight at 4°C with the following primary antibodies: NF-κB (p65) (10745-1-AP, Proteintech), p-NF-κB (Ser536) (#3033, Cell Signaling), IκBα (10268-1-AP，Proteintech Group), p-IκBα (#2859, Cell Signaling), and β-actin (TA-09, Zhongshan Golden Bridge Biotechnology, Beijing, China). Membranes were incubated with secondary antibodies, and signals were visualized using enhanced chemiluminescence.
Nf κb p65 10745 1 ap
Manufactured by Proteintech
Sourced in China
NF-κB (p65) (10745-1-AP) is a primary antibody that detects the p65 subunit of the NF-κB transcription factor complex. NF-κB is a key regulator of genes involved in immune and inflammatory responses, cell growth and survival. This antibody can be used in various applications such as western blotting, immunohistochemistry, and immunofluorescence to study the expression and localization of the p65 subunit.
Automatically generated - may contain errors
Lab products found in correlation
P iκbα, by Cell Signaling Technology (1 mentions)
P nf κb ser536, by Cell Signaling Technology (1 mentions)
β actin, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Gapdh ap0063, by Bioworld Technology (1 mentions)
Laminb1 12987 1 ap, by Proteintech (1 mentions)
Valeric acid, by Merck Group (1 mentions)
Isobutyric, by Merck Group (1 mentions)
Butyric, by Merck Group (1 mentions)
Nrf2 16396 1 ap, by Proteintech (1 mentions)
Ho 1 10701 1 ap, by Proteintech (1 mentions)
Keap1 10503 2 ap, by Proteintech (1 mentions)
β actin gb11001, by Wuhan Servicebio Technology (1 mentions)
3 protocols using nf κb p65 10745 1 ap
1
Spinal Cord Injury Signaling Pathways
2
Catalpol Modulates Inflammatory Pathways
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Catalpol (99% purity) was obtained from Nanjing King Bamboo Biological Technology Co., Ltd. (Nanjing, China), and fetal bovine serum was purchased from Hyclone. The GAPDH (AP0063) was purchased from Bioworld (Jiangsu, China), IL-1 beta (26048-1-AP), IL-6 (66146-1-lg), MMP3 (17873-1-AP), MMP13 (18165-1-AP), IkB Alpha (10268-1-AP), Lamin B1 (12987-1-AP), and NF-κB p65 (10745-1-AP) were purchased from Proteintech (Wuhan, China), NOD2 (DF12125) was purchased from Affinity (Jiangsu, China), and JNK(ET1601-28), P-JNK(ET1609-42), ERK(ET1601-29), P-ERK(ET1610-13), P38(ET1602-26), and P-P38(ER2001-52) were purchased from HUABIO (Zhejiang, China), and all other chemicals were purchased from Sigma.
3
Ferulic Acid Modulates Nrf2/Keap1/HO-1 and NF-κB Signaling
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Ferulic acid (FA, purity ≥ 98 %) was obtained from Dalian Meilun Biotechnology (Dalian, China). Acetic, propionic, butyric, isobutyric, isovaleric, and valeric acid standards were provided by the Sigma-Aldrich Chemical Co. Ltd (St. Louis, MO, USA).
The antibodies of Nuclear factor E2-related factor 2 (Nrf2,16396-1-AP), Kelch-like ECH-associated protein 1(Keap1,10503-2-AP), Heme oxygenase-1(HO-1,10701-1-AP), and Nuclear factor kappa B p65 (NF-κB p65,10745-1-AP) were purchased from Proteintech (Wuhan, China). p38 MAPK (A0227), Phospho-p38 MAPK (P-p38, AP0057), Phospho-Nuclear factor kappa B p65 (P-p65, AP0446) were purchased from ABclonal (Wuhan, China). The β-actin (GB11001) was the production of Servicebio (Wuhan, China).
The antibodies of Nuclear factor E2-related factor 2 (Nrf2,16396-1-AP), Kelch-like ECH-associated protein 1(Keap1,10503-2-AP), Heme oxygenase-1(HO-1,10701-1-AP), and Nuclear factor kappa B p65 (NF-κB p65,10745-1-AP) were purchased from Proteintech (Wuhan, China). p38 MAPK (A0227), Phospho-p38 MAPK (P-p38, AP0057), Phospho-Nuclear factor kappa B p65 (P-p65, AP0446) were purchased from ABclonal (Wuhan, China). The β-actin (GB11001) was the production of Servicebio (Wuhan, China).
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