Dichloromethane dcm

Chloroquine Diphosphate (CQ), Poloxamer 407 (Pluronic^® F-127), and Poloxamer 188 (Pluronic^® F-68) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). d,l-poly(lactic acid) (PLA) (inherent viscosity 0.67 dL g⁻¹ at 25 °C) was purchased from LACTEL Absorbable Polymers^® (Birmingham, AL, USA). Dichloromethane (DCM) (dielectric constant (ε) of 9.1), ethanol (EtOH) (ε of 24.6), acetone (ACE) (ε of 20.6) and N-Methyl-2-pyrrolidone (NMP) were purchased from Labsynth^® (São Paulo, Brazil). The purified water (1.3 µS cm⁻¹) was prepared from reverse osmosis purification equipment, model OS50 LX, Gehaka (Sao Paulo, Brazil). All other reagents have analytical grade.

Benznidazole (BNZ) (Roche^®, São Paulo, Brazil) was donated by LAFEPE^®®—Pharmaceutical Laboratory of Pernambuco (Recife, Brazil); poly vinyl alcohol (PVA) with viscosimetric molecular mass of 4.7 × 10⁴ g/mol (Vetec^®, São Paulo, Brazil); copolymer of Polymethylmethacrylate (PMMA), Eudragit^®® E PO (EUD) was purchased from Pharma Polymers (Röhm GmbH & Co. KG, Darmstadt, Germany); SA (N-Acetylneuraminic acid, Neu5Ac, >98%, CarboSynth, Compton, Berkshire, England) and Chol (92.5%, Vetec^®®, Sigma-Aldrich, Saint Louis, MO, EUA). The organic solvents ethanol (EtOH) (ɛ of 24.6), acetone (ACE) (ɛ of 20.6), and dichloromethane (DCM) (dielectric Constant (ɛ) of 9.1) were purchased from Labsynth^®® (São Paulo, Brazil). Both 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and dimethyl sulfoxide (DMSO) were purchased from Sigma Aldrich (Saint Louis, CA, USA). The purified water (1.3 μS) was prepared by equipment model OS50 LX (Gehaka, SP, Brazil) from reverse osmosis purification. All other chemicals and reagents were of analytical grade.