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Taqman 48 analyzer

Manufactured by Roche
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The TaqMan® 48 Analyzer is a real-time PCR (Polymerase Chain Reaction) system designed for gene expression analysis and detection of nucleic acids. It is capable of processing up to 48 samples simultaneously, utilizing TaqMan® technology for accurate and reliable results.

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Lab products found in correlation

Ampliprep instrument, by Roche (2 mentions) Ampliprep, by Roche (1 mentions) Cobas 8000 analyzer, by Roche (1 mentions) Facscan 2, by BD (1 mentions) Taqman analyzer, by Roche (1 mentions) Fast sybr green master mix, by Thermo Fisher Scientific (1 mentions) Purelink genomic dna mini kit, by Thermo Fisher Scientific (1 mentions) Steponeplustm, by Thermo Fisher Scientific (1 mentions)

6 protocols using taqman 48 analyzer

1

HIV-1 Viral Load Analysis Using COBAS Assay

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Human plasma collected in EDTA anticoagulant was used for viral load analysis. HIV-1 viral load testing was performed using the COBAS®Ampliprep/ COBAS®Taqman® 48 HIV-1 Test. The specimen was prepared using the automated COBAS® Ampliprep Instrument with amplification and detection was done using the COBAS® TaqMan® 48 Analyzer as per manufacturer’s instructions.
Luchen C.C., Mwaba J., Ng’ombe H., Alabi P.I., Simuyandi M., Chilyabanyama O.N., Hatyoka L.M., Mubanga C., Bosomprah S., Chilengi R, & Chisenga C.C. (2021). Effect of HIV status and retinol on immunogenicity to oral cholera vaccine in adult population living in an endemic area of Lukanga Swamps, Zambia. PLoS ONE, 16(12), e0260552.
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2

Comprehensive Blood Analysis of HIV/AIDS Patients

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A 2ml sample of venous blood was collected from each HIV/AIDS patient on an empty stomach at each time point. Blood cells were analyzed by flow cytometry (FACSCan II, BD Biosciences, San Jose, CA) using a combined CD3/CD4/CD8/CD45 Multitest reagent (BD Biosciences, San Jose, CA), allowing the absolute number of lymphocyte subsets to be measured and analyzed. All tests were completed less than 4 hours after venous blood collection. White blood cell (WBC) and platelet counts and hemoglobin (Hb) concentration were measured by routine blood testing using a Nisen Meikang automatic hematocyte counter (Japan). Total cholesterol (TC), total triglyceride (TG), alanine transaminase (ALT), aspartate aminotransferase (AST), and creatinine levels, aspects of blood lipids, and liver and renal function tests were performed using a Roche Cobas 8000 analyzer. Samples were prepared using a High Pure System Viral Acid kit, while a COBAS TaqMan 48 analyzer was used for automatic amplification and measurement. Samples were tested after routine daily indoor quality control testing.
Li B., Li M., Song Y., Lu X., Liu D., He C., Zhang R., Wan X., Zhang R., Sun M., Kuang Y.Q, & Li Y. (2022). Construction of Machine Learning Models to Predict Changes in Immune Function Using Clinical Monitoring Indices in HIV/AIDS Patients After 9.9-Years of Antiretroviral Therapy in Yunnan, China. Frontiers in Cellular and Infection Microbiology, 12, 867737.
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3

COBAS TaqMan 48 Analyzer Result Validation

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After the COBAS TaqMan 48 Analyzer run, the results will be checked for flags or error messages in the result report. Specimens with flags and comments would be interpreted as described in the results section. After accepting, the results would be stored in the data archive. All the used K-tubes would be removed from the COBAS TaqMan 48 Analyzer.
The results would be validated or invalidated depending on whether there is an appearance of a flag or not on any of the controls [HBV L (+) C, v2.0, and CTM (−) C].
Eleje G.U., Mbachu I.I., Ogwaluonye U.C., Kalu S.O., Onubogu C.U., Nweje S.I., Uzochukwu C.E., Nwankwo C.H., Fiebai P.O., Loto O.M., Akaba G.O., Usman H.A., Rabiu A., Egeonu R.O., Igue O.E., Adesoji B.A., Jibuaku C.H., Aja P.O., Chidozie C.P., Ibrahim H.S., Aliyu F.E., Numan A.I., Okoro O.D., Omoruyi S.A., Oppah I.C., Anyang U.I., Ahmed A., Chukwurah S.N., Umeononihu O.S., Chukwuanukwu R.C., Umeh E.O., Emeka E.A., Ogbuagu C.N., Yakasai I.A., Ezechi O.C, & Ikechebelu J.I. (2020). Prevalence, seroconversion and mother-to-child transmission of dual and triplex infections of HIV, hepatitis B and C viruses among pregnant women in Nigeria: study protocol. Reproductive Health, 17, 144.
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4

Quantitative HCV-RNA PCR Identification

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Identification of HCV infection was confirmed by quantitative HCV- RNA PCR using COBAS® AmpliPrep/COBAS® TaqMan® HCV Quantitative Test, version 2.0 (lower limit of detection, 15 IU/mL), according to the manufacturer’s instructions. This test is used for quantifying HCV RNA genotypes 1 to 6 in human EDTA plasma or serum using the COBAS® AmpliPrep Instrument for automated specimen processing and the COBAS® TaqMan® Analyzer or the COBAS® TaqMan® 48 Analyzer for automated amplification and detection of HCV RNA.
Hawsawi N.M., Saber T., Salama H.M., Fouad W.S., Hagag H.M., Alhuthali H.M., Eed E.M., Saber T., Ismail K.A., Al Qurashi H.H., Altowairqi S., Samaha M, & El-Hossary D. (2023). Genotypes of Hepatitis C Virus and Efficacy of Direct-Acting Antiviral Drugs among Chronic Hepatitis C Patients in a Tertiary Care Hospital. Tropical Medicine and Infectious Disease, 8(2), 92.
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5

Quantifying Intracellular HBV-DNA and Secreted HBsAg

Cited 1 time
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To determine intracellular HBV-DNA quantities, total DNA was extracted from HBV-infected Huh7/NTCP cells using PureLink® Genomic DNA Mini Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. Total DNA was diluted to 10 ng/μL. Real-time qPCR was carried out using Fast SYBR® Green Master Mix (Thermo Fisher Scientific), and fluorescent signals were analyzed using StepOnePlusTM. The primers used in the reactions were as follows: 5′-CCTCTGCCTAATCATCTCATGTTC-3′ (forward) and 5′-CGGTGTCGAGGAGATCTCGAATAG-3′ (reverse) for HBV; and 5′-CCATGCCATCACTGCCACCC-3′ (forward) and 5′-GCCAGTGAGCTTCCCGTTCAG-3′ (reverse) for GAPDH, as an internal control [37 (link)]. Each 20 μL reaction mixture consisted of 10 ng/μL DNA (2 μL), 50 μM each forward and reverse primers (0.12 μL), RNase-free water (7.76 μL), and Fast SYBR® Green Master Mix 10 μL. The final concentration of each primer was 300 μM.
HBV-DNA levels in culture medium supernatants were determined using Cobas® TaqMan® 48 Analyzer. For sample preparation, a culture medium supernatant of 0.35 mL/sample was diluted with 1.55 mL PBS to obtain a total volume of 1.9 mL.
HBsAg levels in the culture medium supernatants were measured using the ARCHITECTTM i2000.
Fujita K., Mimura S., Iwama H., Nakahara M., Oura K., Tadokoro T., Nomura T., Tani J., Yoneyama H., Morishita A., Oryu M., Himoto T., Nishitsuji H., Shimotohno K., Omata M, & Masaki T. (2018). Serum miRNAs Predicting Sustained HBs Antigen Reduction 48 Weeks after Pegylated Interferon Therapy in HBe Antigen-Negative Patients. International Journal of Molecular Sciences, 19(7), 1940.
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6

Automated HBV DNA Quantification

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The COBAS TaqMan Analyzer 48 or 96 Analyzer will be started within 120 min following completion of specimen and control preparation. The COBAS TaqMan 48 analyzer automatically determines the HBV DNA concentration for the specimens and controls. The DNA concentration will be expressed in copies/mL. The Cycle Threshold value (Ct) for the HBV DNA and the HIV QS DNA will be determined. The HBV DNA concentration based upon the Ct values for the HBV DNA and the HIV-IQS DNA and the lot-specific calibration coefficients provided on the cassette barcodes would also be determined.
Eleje G.U., Mbachu I.I., Ogwaluonye U.C., Kalu S.O., Onubogu C.U., Nweje S.I., Uzochukwu C.E., Nwankwo C.H., Fiebai P.O., Loto O.M., Akaba G.O., Usman H.A., Rabiu A., Egeonu R.O., Igue O.E., Adesoji B.A., Jibuaku C.H., Aja P.O., Chidozie C.P., Ibrahim H.S., Aliyu F.E., Numan A.I., Okoro O.D., Omoruyi S.A., Oppah I.C., Anyang U.I., Ahmed A., Chukwurah S.N., Umeononihu O.S., Chukwuanukwu R.C., Umeh E.O., Emeka E.A., Ogbuagu C.N., Yakasai I.A., Ezechi O.C, & Ikechebelu J.I. (2020). Prevalence, seroconversion and mother-to-child transmission of dual and triplex infections of HIV, hepatitis B and C viruses among pregnant women in Nigeria: study protocol. Reproductive Health, 17, 144.
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