Mouse sciatic nerves were dissected and fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer. Nerve samples were then osmicated, dehydrated, and embedded in araldite resin. Transverse nerve sections (1 µm) were cut on a Leica RM2065 microtome and stained with methylene blue Azure II. Images were collected on a Leica DMR microscope or an Olympus BX61 microscope. Axon numbers were determined from two non-overlapping fields (50×50 µm) from each of three mutant and three control nerve samples. Axon diameters were measured by Image J.
Dmr microscope
Manufactured by Olympus
Sourced in Germany
The DMR microscope is a high-performance optical microscope designed for laboratory use. It features a compact and ergonomic design, providing users with a stable and reliable platform for various imaging applications. The DMR microscope offers a range of objective lenses, allowing for a wide variety of magnification levels to suit different specimen requirements. Its core function is to provide clear and detailed visualization of samples, enabling researchers and technicians to observe and analyze their subjects with precision.
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Lab products found in correlation
4 protocols using dmr microscope
1
Quantitative Histological Analysis of Mouse Sciatic Nerve
2
Coronal Brain Imaging Protocol
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Coronal brain sections were imaged on a Leica DMR microscope (Wetzlar, Germany) with an Olympus DP70 digital camera (Shinjuku, Tokyo, Japan). Each coronal brain section was imaged using a 10x objective, then stitched together using Adobe Photoshop CC15 (Adobe, San Jose, CA). The corresponding anterior-posterior levels of the coronal brain slices were identified using the Paxinos and Watson brain atlas55 .
3
GUS Staining in U. gibba Tissues
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Transgenic and nontransgenic U. gibba tissues were incubated in GUS reaction buffer (0.5 mg/mL of 5‐bromo‐4‐chloro‐3‐indolyl‐b‐d ‐glucuronide in 100 mM sodium phosphate, pH 7.0) overnight at 37 °C. The tissues were cleared by the Malamy and Benfey method [48 (link)] and observed and photographed using Nomarski optics in a Leica DMR microscope and a SZH10 Olympus stereomicroscope.
4
Quantitative Histological Analysis of Mouse Sciatic Nerve
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Mouse sciatic nerves were dissected and fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer. Nerve samples were then osmicated, dehydrated, and embedded in araldite resin. Transverse nerve sections (1 µm) were cut on a Leica RM2065 microtome and stained with methylene blue Azure II. Images were collected on a Leica DMR microscope or an Olympus BX61 microscope. Axon numbers were determined from two non-overlapping fields (50×50 µm) from each of three mutant and three control nerve samples. Axon diameters were measured by Image J.
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