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35 protocols using poly sodium 4 styrenesulfonate

1

Synthesis and Characterization of Polymer Solutions

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Poly(diallyldimethylammonium
chloride) (PDADMAC, 30 wt % in water, Mw ≈ 200–350 kDa) solution and poly(sodium 4-styrenesulfonate)
(PSS, 25 wt % in water, Mw ≈ 200
kDa) were purchased from Merck (The Netherlands). Polyelectrolyte
solutions were used directly without further purification. RTV-615
A (Permacol B.V, Ede, The Netherlands), prepolymer (1020 kg/m3) and RTV-615 B (Permacol B.V, Ede, The Netherlands), and
curing agent (990 kg/m3) were used to produce polydimethylsiloxane
(PDMS). Sodium chloride (NaCl) (99.96%) was obtained from AkzoNobel
(The Netherlands). PS-FluoRed-1.0; 1.14 μm (2.5 wt %, SD = 0.03
μm, abs/em = 530/607 nm) and PS-RhB-PEG-Fi70–1: 1.09
μm (2.5 wt %, SD = 0.04 μm, abs/em = 560/584 nm) were
obtained from Microparticles GMBH (Berlin, Germany). FluoSpheres PS-carboxylate;
1.00 μm (2 wt %, SD = 0.02 μm, abs/em = 580/605 nm) was
obtained from Thermo Fisher (United States).
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2

Polyelectrolyte Complex Formation

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Poly(sodium 4-styrenesulfonate) (PSS, Mw ∼ 200 kDa, 30 wt% in water), poly(diallyldimethylammonium chloride) (PDADMAC, Mw ∼ 350 kDa, 20 wt% in water), poly(ethylene glycol) (PEG) of various molecular weights (200, 400, 600, 1000, 1500, and 2000 Da) were purchased from Merck. Magnesium sulfate (MgSO4), sodium sulfate (Na2SO4), magnesium chloride (MgCl2), potassium chloride (KCl) and 2-propanol (IPA) were purchased from Sigma Aldrich. Sodium chloride (NaCl, pharmaceutical grade, SanalP) was kindly supplied by Akzo Nobel.
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3

Polyelectrolyte Complexes and Gold Nanorods

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Poly(sodium 4-styrenesulfonate) [PSS (MW ≈ 75,000, 18 wt% in water)] and poly(allylamine hydrochloride) [PAH (MW ≈ 15,000)], Figure S1, were obtained from Sigma-Aldrich (Steinheim, Germany). Trifluoroacetic acid (TFA) and sodium hydroxide (NaOH) used to control the pH were also purchased from Sigma-Aldrich. CaCl2 (purity ≥ 99.5%)] was obtained from Fisher Chemical (Waltham, MA, USA) and Na2CO3 was obtained from J. T. Baker (Philadelphia, PA, USA). TMPyP tetraiodide salt was purchased from Sigma-Aldrich. Gold nanorods (CTAB stabilized, 25 × 71 nm, product number A12-25-650-CTAB-DIH-25 lot RPD236AD) were purchased from Nanopartz Inc., Ltd. (Loveland, CO, USA). All the reagents were used as received. Polyelectrolyte solutions (3 mg/mL of PSS and 12 mg/mL of PAH, 0.5 M NaCl) were prepared in bi-distilled water, and the pH was adjusted to 6.5 for the PSS/PAH used in the PEC’s preparation. PSS solution (1.7 mg/mL) was used for nanorod functionalization. Glass microscope slides (0.13–0.17 mm thickness) were obtained from Normax, Marinha Grande, Portugal.
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4

Multifunctional Hydrogel Biosensor Fabrication

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PEG tetra-vinyl sulfone (10 kDa, PEG-VS) and PEG tetra-thiol (10 kDa, PEG-SH) purchased from JenKem Technology, USA. Oxygen-sensitive phosphor Pd(II) meso-tetra(sulfophenyl) tetrabenzoporphyrin sodium salt (S-PdBP) was purchased from Frontier Specialty Chemicals, USA. Glucose oxidase (≤ 40 U/mg) from Aspergillus niger, (Z,Z,Z)-Sorbitan tri-9-octadecenoate (SPAN 85), and polyoxyethylenesorbitan trioleate (TWEEN 85) were purchased from Tokyo Chemical Industry, USA. Catalase from bovine liver (2,000-5,000 U/mg), alginic acid sodium salt from brown algae (viscosity of ~250 cps at 2% solution, average Mw 75,000-100,000 Da), poly(allylamine hydrochloride) (PAH) (average Mw 17,500 Da), poly(Sodium 4-styrenesulfonate) (PSS) (average Mw ~70,000 Da), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIZMA base), triethanolamine (TEOA), 2-(N-morpholino) ethane-sulfonic acid sodium salt (MES sodium salt), and calcium carbonate were purchased from Sigma Aldrich Chemical Co., St. Louis, MO, USA. Isooctane, dimethyl sulfoxide (DMSO), tris(hydroxymethyl) aminomethane (TRIS) hydrochloride, and phosphate buffered saline (PBS) tablets were purchased from Avantor Performance Materials, LLC, USA. Calcium chloride dihydrate was purchased from VWR Chemicals, LLC, OH, USA. All reagents were used as received without further purification unless mentioned otherwise.
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5

Rhodamine B-Functionalized Carbon Nanotubes

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Rhodamine B isothiocyanate (70% labeling efficiency), (3-aminopropyl) triethoxysilane (97%), 4-(dimethylamino)pyridine (≥99%), dicyclohexylcarbodiimide solution (≥99%), triethylamine BioUltra ≥99.5%, poly(diallyldimethylammonium chloride) (MW <100,000 Da), poly-(sodium 4 styrenesulfonate) (MW =70,000 Da), nitric acid (HNO3, 65%), sodium hydroxide (NaOH, ≥97%), tetraethyl orthosilicate (TEOS, 98%), and EtOH were obtained from Sigma-Aldrich Química SL (Madrid, Spain). Multiwalled carbon nanotubes 9.5 nm in diameter and 1.5 μm in length, 95% C purity, syn thesized using Catalytic Chemical Vapor Deposition process, were purchased from Nanocyl as a powder. Ammonium hydroxide solution (NH4OH, 28%–30% NH3), cystamine hydrochloride 97%, N,N-dimethylformamide were obtained from Fluka. Sulfuric acid (H2SO4, 96%) was purchased from Panreac and sodium chloride (NaCl, ≥99%) from Merck.
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6

2D Photonic Crystal Cavity Fabrication

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Sulfuric acid, hydrogen peroxide, acetone, and 2-propanol were purchased from Wako Pure Chemicals (Osaka, Japan) and used for cleaning the wafer. ZEP520A (ZEON Corp., Tokyo, Japan) and xylene (Wako Pure Chemicals, Osaka, Japan) were used for fabricating the 2D-PhC cavity.
For optical characterization, poly(allylamine hydrochloride) (PAA) and poly(sodium 4-styrene sulfonate) (PSS), purchased from Sigma-Aldrich (St. Louis, MO), were used.
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7

Polyelectrolyte Complexes Formation

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Poly-L-lysine hydrobromide (Mw 24 kDa), poly-L-lysine -FITC labelled (Mw 15-30 kDa), poly(ethyleneimine) (Mw 750 kDa), Poly(allylamine hydrochloride) (Mw 56 kDa), poly(sodium 4-styrenesulfonate) (Mw 70 kDa), Tris base and sodium chloride were purchased from Sigma, Germany and used without further purification. Dried sodium hyaluronate (Mw 360 kDa) was from Lifecore Biomedical, USA and hydrochloric acid from Merck, Germany.
The water used in all experiments was prepared in a three-stage Millipore Milli-Q Plus 185 purification system and had a resistivity higher than 18.2 MΩ cm. The 12mm (diameter) round coverslips (Size No. 1) were purchased from Marienfeld GmbH, Germany and Hellmanex II from Hellma Analytics, Germany.
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8

Synthesis of Plasmonic Nanoparticles

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Hexadecyltrimethylamonium
bromide (CTAB, ≥ 98%) was purchased from Tokyo Chemical Industry.
Hydrogen tetrachloroaurate solution (HAuCl4, 30 wt %),
sodium borohydride (NaBH4, ≥ 98%), silver nitrate
(AgNO3, ≥ 99.0%), sodium bromide (NaBr, ≥
99,99%), l-ascorbic acid (C6H8O6, ≥ 99%), poly(sodium 4-styrenesulfonate) (NaPSS, Mw
70 kDa), sodium citrate (citNa, > 99%), thiol-polyethylene glycol-carboxylic
acid (thiol-PEG, > 99%), sodium chloride (≥ 99%), sodium
fluorescein (fluorescent tracer grade), and sodium hydroxide (NaOH,
≥ 97%) were obtained from Sigma-Aldrich, Singapore. H2O2 solution (30–32 wt %) was acquired from QüreC
(Quality Reagents Chemical, New Zealand), while hydrochloric acid
(HCl, 36–38 wt %, Duskan Pure Chemical) was acquired from Duskan
Reagents, Singapore. Polyurethane (1 mm thick, Swees Engineering Co.,
pte, PU) and crystal violet (≥ 99%, Certistain, CV) were
obtained from MERK, Singapore; Nutrient Agar (NA) CM0003 was obtained
from Oxoid).
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9

Synthesis and Characterization of Zinc-Based Photosensitizers

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Zinc acetate (99%), thiourea (AR, 99%), indocyanine green (ICG), iron (III) chloride (FeCl3) and 1,3-diphenylisobenzofuran (DPBF) were purchased from Aladdin Co. Ltd. Polyvinylpyrrolidone (K30), Zinc nitrate hexahydrate (AR), methanol anhydrous, sulfuric acid and sodium acetate anhydrous were obtained from Sinopharm Chemical Reagent Co. Ltd. Tirapazamine and CCK-8 were supplied by Dalian Meilun Biotechnology Co. Ltd. Poly (sodium 4-styrenesulfonate) (PSS, Mw = ~70000), 2-methylimidazole, N, N-Dimethyl-p-phenylenediamine (≥99.0%) and dichlorofluorescein diacetate (DCFH-DA, ≥97%) were purchased from Sigma-Aldrich Co. Ltd.
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10

Fabrication of Colloidal Polymer Films

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Glass substrates (3.5 × 2.5 cm2) were obtained from Electro Optical Technologies. Polystyrene spheres with diameter of 80 ± 7 nm (sulfate latex with surface charge density 1.2 µC/cm2 and concentration 8% w/v) and 500 ± 50 nm (non-functionalized Polybeads® – microspheres containing a slight anionic charge from sulfate ester, concentration 2.5% w/v) were respectively purchased from Invitrogen and Polyscience, Inc. Poly(diallyldimethylammonium chloride) (PDDA, MW 200000–350000), poly(sodium 4-styrenesulfonate) (PSS, MW 70000) and poly(allylamine hydrochloride) (PAH, MW 50000) were purchased from Sigma-Aldrich.
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