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The VR682 is a laboratory equipment designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cells. The core function of the VR682 is to maintain consistent temperature, humidity, and gas levels within the cell culture chamber.

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Lab products found in correlation

Ccl 158, by American Type Culture Collection (2 mentions) Ccl 81, by American Type Culture Collection (1 mentions) Htb 96, by American Type Culture Collection (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Nahco3, by Thermo Fisher Scientific (1 mentions)

4 protocols using vr682

1

Propagation of GPCMV and HSV-1 Viruses

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GPCMV (strain 22122, ATCC VR682), first and second-generation GPCMV BAC derived viruses were propagated on guinea pig fibroblast lung cells (GPL; ATCC CCL-158™) as previously described. High titer stock was generated as previously described. Vero cells (ATCC; CCL-81™) and human osteosarcoma U2OS cells (ATCC; HTB-96™) were cultured in high glucose DMEM medium supplemented with 10% fetal calf serum (FCS). WT HSV-1 (17+strain) virus and ICP0Δ mutant virus stocks were generated on U2OS cells. Oligonucleotides were synthesized by Sigma-Genosys (The Woodlands, TX) (Table S2).
Hornig J., Choi K.Y, & McGregor A. (2017). The essential role of guinea pig cytomegalovirus (GPCMV) IE1 and IE2 homologs in viral replication and IE1-mediated ND10 targeting. Virology, 504, 122-140.
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2

Propagation and titration of GPCMV strains

Cited 2 times
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GPCMV (strain 22122, ATCC VR682), and second generation GPCMV BAC (34 (link), 62 (link)) derived viruses were propagated on guinea pig fibroblast lung cells (GPL; ATCC CCL 158) in F-12 medium supplemented with 10% fetal calf serum (FCS, Life Technologies), 10,000 IU of penicillin/liter, 10 mg of streptomycin/liter (Life Technologies), and 7.5% NaHCO3 (Life Technologies) at 37°C/5% CO2. Virus titrations were carried out on six-well plates. Plaques were visualized by fluorescence microscopy. High titer stock viruses were generated as previously described (68 (link)). Viruses were also propagated in renal and trophoblast epithelial cells for tropism studies (67 (link)). All virus titrations were carried out on GPL fibroblast cells. Viral growth studies were performed using an input virus moi of 1 pfu/cell. Time points were taken every 24h, up to 10 days post infection and titrated in duplicate on GPL cells. Oligonucleotides were synthesized by Sigma-Genosys (The Woodlands, TX) and are listed in supplemental table 1 (Table S1).
Coleman S., Choi K.Y, & McGregor A. (2017). Cytomegalovirus UL128 homolog mutants that form a pentameric complex produce virus with impaired epithelial and trophoblast cell tropism and altered pathogenicity in the guinea pig. Virology, 509, 205-221.
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3

GPCMV Propagation and Recombinant Adenovirus

Cited 3 times
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GPCMV (strain 22122, ATCC VR682) and second generation GPCMV BAC [75 (link),76 (link)] derived viruses were propagated on guinea pig fibroblast lung cells (GPL; ATCC CCL 158) or guinea pig renal epithelial cells (REPI), and virus titrations were carried out on 12-well plates of GPL cells as previously described [36 (link)]. Recombinant defective adenovirus (Ad5) vectors encoding individual glycoproteins (gB, gH, gL, GP129, GP131, GP133) were previously described [24 (link),25 (link),28 (link)]. All oligonucleotides were synthesized by Sigma-Genosys (The Woodlands, TX, USA).
Choi K.Y, & McGregor A. (2021). A Fully Protective Congenital CMV Vaccine Requires Neutralizing Antibodies to Viral Pentamer and gB Glycoprotein Complexes but a pp65 T-Cell Response Is Not Necessary. Viruses, 13(8), 1467.
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4

Minimally Adapted GPCMV Virus Propagation

Cited 1 time
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A minimally tissue culture adapted stock of GPCMV 22122 (ATCC VR-682), prepared as previously described by passaging guinea pig salivary gland homogenate twice on JH4 guinea pig lung fibroblasts (ATCC CCL-158), was used for this study (33 (link), 45 (link), 46 (link)). JH4 cells were purchased from ATCC and propagated according to their specifications excepting that the growth media was supplemented with sodium bicarbonate (47 ). GPCMV stocks were prepared as previously described and titered on JH4 cells (48 (link)). For animal studies, virus was aliquoted into single-use units, flash-frozen, and stored at -80°C. These aliquots were routinely re-titered as guinea pigs were infected to confirm that the stock did not deteriorate during storage.
Berkebile Z.W., Putri D.S., Abrahante J.E., Seelig D.M., Schleiss M.R, & Bierle C.J. (2021). The Placental Response to Guinea Pig Cytomegalovirus Depends Upon the Timing of Maternal Infection. Frontiers in Immunology, 12, 686415.
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