For multicolor flow cytometric analyses, the following fluorochrome-labeled Ab and IgG subset controls (all purchased from Becton-Dickinson (BD, Franklin Lakes, NJ, unless otherwise indicated) were utilized for surface or intracellular staining of cells: CD3-Alexa Fluor 700 (clone UCHT1), CD3-PerCPCy5.5 (5k7), CD4-V450 (RPA-T4), CD4-PE (RPA-T4), CD4-FITC (RPA-T4), CD4-V500 (RPA-T4), CD25-BV605 (2A3), CD25-PE (2A3), CD127-PE (hIL-7R-M21), CD127-PErCPCy5.5 (hIL-7R-M21), CD196-BV605 (11A9), IL-17A-Alexa Fluor 700 (N49-653), RORγt-PE (Q21-555), FoxP3-V450 (259D/C7). Foxp3-APC (PCH101) was a component of the Anti-Human Foxp3 Staining Set (eBioscience, San Diego, CA). FacsLysis was purchased from BD. The following reagents were purchased from Sigma-Aldrich, Inc. (St. Louis, MO): RPMI-1640, phosphate-buffered saline (PBS), fetal bovine serum, paraformaldehyde, phorbol 12-myristate 13-acetate, ionomycin, brefeldin A.
Il 17a alexa fluor 700
Manufactured by BD
The IL-17A-Alexa Fluor 700 is a fluorescently-labeled antibody that specifically binds to the cytokine interleukin-17A (IL-17A). It is designed for use in flow cytometry and other immunoassay applications to detect and quantify IL-17A-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 pe, by BD (2 mentions)
Cd3 percp cy5, by BD (2 mentions)
Cd127 percp cy5, by BD (2 mentions)
Facs lysis, by BD (2 mentions)
Rpmi 1640, by Merck Group (2 mentions)
Cd127 pe, by BD (2 mentions)
Cd3 alexa fluor 700, by BD (2 mentions)
Cd4 v450, by BD (2 mentions)
Cd4 fitc, by BD (2 mentions)
Cd25 pe, by BD (2 mentions)
Cd25 bv605, by BD (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Ionomycin, by Merck Group (2 mentions)
Brefeldin a, by Merck Group (2 mentions)
Foxp3 apc pch101, by Thermo Fisher Scientific (2 mentions)
Anti human foxp3 staining set, by Thermo Fisher Scientific (2 mentions)
Cd196 bv605, by BD (2 mentions)
Cd4 v500, by BD (2 mentions)
Foxp3 v450, by BD (2 mentions)
2 protocols using il 17a alexa fluor 700
1
Multicolor Flow Cytometry Analysis
2
Multicolor Flow Cytometry Analysis
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