Cells were centrifugated during 10 min at 377 g and fixed in an alcohol based fixative solution Thinprep® (Hologic) during 15 min. After centrifugated during 5 min at 377 g, the cells were resuspended in 10 ml of Epredia™ Gel (Richard-Allan Scientific™ HistoGel™). The gel was hardened during 15 min at 4 °C and the corresponding blocks were dehydrated and embedded in paraffin. 3 µm-sections were immunostained using an antibody anti-histone H3 containing the trimethylated lysine 27 (H3K27me3) (Diagenode). Heat induced antigen retrieval was done using CC1 basic buffer (Ventana). Staining was performed using DAB Ultraview detection system (Ventana).
Cc1 basic buffer
Manufactured by Roche
The CC1 basic buffer is a laboratory buffer solution designed for use in various biochemical and molecular biology applications. It serves as a standard buffer to maintain pH and ionic conditions required for various experimental procedures. The product details and intended use are presented in a factual and unbiased manner without any interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cc1 basic buffer
1
Histone H3 Lysine 27 Trimethylation Immunohistochemistry
2
Immunohistochemical Analysis of H3K27me3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were centrifugated during 10 min at 377g and fixed in an alcohol based fixative solution Thinprep® (Hologic) during 15 min. After centrifugated during 5 min at 377g, the cells were resuspended in 10ml of Epredia™ Gel (Richard-Allan Scientific™ HistoGel™). The gel was hardened during 15 min at 4°C and the corresponding blocks were dehydrated and embedded in paraffin. 3µm-sections were immunostained using an antibody anti-histone H3 containing the trimethylated lysine 27 (H3K27me3) (Diagenode). Heat induced antigen retrieval was done using CC1 basic buffer (Ventana).
Staining was performed using DAB Ultraview dection system (Ventana).
Staining was performed using DAB Ultraview dection system (Ventana).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!