Cetuximab was purchased from Merck (Darmstadt, Germany), and RSL3 (HY-100218A) and SB202190 (HY-10295) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Necrostatin-1 (GC11008) and Z-VAD-FMK (GC12861) were purchased from GLPBIO (Montclair, CA, USA). Ferrostatin-1 (Fer-1) (HY-100579) and 3-MA (HY-19312) were purchased from MedChemExpress. Primary antibodies against Keap1 (AF5266), HO-1 (AF5393), p38 (BF8015), and phosphorylated (p)-P38 (AF4001) were purchased from Affinity Biosciences (OH, USA). A Nrf2 antibody (16396-1-AP) was purchased from Proteintech (Rosemont, IL, USA). A Ki67 antibody (ab15580) was purchased from Abcam (Cambridge, United Kingdom).
Ki 67 antibody ab15580
Manufactured by Abcam
Sourced in United States
The Ki-67 antibody (ab15580) is a laboratory tool used to detect the Ki-67 protein, a well-established marker of cellular proliferation. The antibody can be used in various immunodetection techniques to identify and quantify proliferating cells within a sample.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Rsl3 hy 100218a, by MedChemExpress (1 mentions)
Sb202190 hy 10295, by MedChemExpress (1 mentions)
3 ma hy 19312, by MedChemExpress (1 mentions)
Ferrostatin 1 fer 1 hy 100579, by MedChemExpress (1 mentions)
Cetuximab, by Merck Group (1 mentions)
Hematoxylin, by Thermo Fisher Scientific (1 mentions)
Cyclin d3 dcs22, by Cell Signaling Technology (1 mentions)
Dab reaction, by Vector Laboratories (1 mentions)
Abc substrate kit, by Vector Laboratories (1 mentions)
Prb s780, by Cell Signaling Technology (1 mentions)
Pan keratin, by Cell Signaling Technology (1 mentions)
Cisplatin p4394, by Merck Group (1 mentions)
β actin sc 47778 antibody, by Santa Cruz Biotechnology (1 mentions)
Streptavidin hrp p0397, by Agilent Technologies (1 mentions)
U plex cytokine assay, by Mesoscale (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Bluing reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
7 protocols using ki 67 antibody ab15580
1
Ferroptosis Induction and Inhibition Assay
2
Immunohistochemical Analysis of Lymphoma Xenografts
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Lymphoma tissues from xenograft were fixed with 4%PFA, dehydrated, and embedded in paraffin followed by sectioned with a microtome. H&E sections and tissue microarray slides (US Biomax, Inc.) were blocked with 10% goat serum, incubated with the primary antibodies and subsequently incubated with biotinylated antibodies. Signal was developed with ABC substrate kit (Vector) followed by DAB reaction (Vector), and counterstained with Hematoxylin (Thermo Scientific). The Ki-67 antibody (ab15580) was purchased from Abcam. Fbxl8 antibody (NBP2-34012) was obtained from Novus Biologicals. Cyclin D3 (DCS22) and pRb (S780) were purchased from Cell Signaling Technology and Santa Cruz, respectively. The IHC score in each core was defined by following formula Intensity = (staining positive population (1 to 3) × staining intensity (1 to3)). The IHC scores 1–2, 3–5, and 6–9 were defined as expression of low, medium, and high, respectively.
3
Cisplatin-Induced EMT Signaling Pathway
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Cisplatin (P4394) was purchased from Sigma (USA). BCA protein assay kit (23225) was purchased from Thermo-Scientific (USA). Nitrocellulose membranes (1060003) were purchased from GE (USA). E-cadherin (#3195), Snail (#3879), Slug (#9585), Vimentin (#5741), p-ERK (#9106), ERK (#4695), and pan-keratin (#4545) antibodies were purchased from Cell signaling technology (USA). β-Actin (Sc-47778) antibody was purchased from Santa Cruz (USA). Ki-67 antibody (ab15580) was purchased from Abcam (USA). IR800 dye-conjugated rabbit (#926-32213) and mouse (#926-32212) secondary antibodies were purchased from Li-COR Biosciences (USA). Secondary antibody labeled with Cy3 (#711-165-152) was purchased from Jackson ImmunoResearch (USA) and Biotin-rabbit IgG (656140) from Invitrogen, and streptavidin-HRP (P0397) from DAKO (USA).
4
DSS-Induced Colitis and Cytokine Analysis
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Dextran sodium sulfate (DSS; molecular weight 36–50 kDa, #160110) was purchased from MP Biomedicals. TRITC-conjugated dextran (4 kDa) was obtained from TdB Consultancy AB. Primary antibodies directed against total AMPKα (#2532), AMPKα phosphorylated at Thr-172 (#2531) and β-actin (#4967) were purchased from Cell Signaling Technology. Ki67 antibody (ab15580) was purchased from Abcam. Circulating cytokines were analyzed using the U-PLEX cytokine assay from Meso Scale Diagnostics.
5
Peptide-Based Targeting of EGFR in HNSCC
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Disruptin, Biotin-NH-YGRKKRRQRRRSVDNPHVC-CO2H, NB-Disruptin (non-biotinylated), NH2-YGRKKRRQRRRSVDNPHVC-CO2H, RI-Disruptin (retroinverso), NH2-cvhpndvsrrrqrrkkrgy-CO2H, and Scram-peptide, Biotin-NH-YGRKKRRQRRRNHVPSDVC-CO2H were synthesized by SynPeptide Co Ltd. EGFR antibody (cat#4267) was acquired from Cell Signaling Technology, Inc. (Danvers, MA). Ki-67 antibody (ab15580) was acquired from Abcam (Cambridge, MA). CD-31 (clone JC70A) was purchased from (Dako, Carpinteria, CA). The human head and neck squamous cell carcinoma (HNSCC) cell lines, UMSCC11B, and UMSCC47 were kindly provided by Dr. Thomas Carey (University of Michigan, Ann Arbor, MI). The lung cancer cell line, NCI-H1975, was provided by J. Engelman (Massachusetts General Hospital, Boston, MA). A549 cells were purchased from the American Type Culture Collection (Manassas, VA). All cell lines were grown in RPMI-1640 medium supplemented with 10% Fetal Bovine Serum (Gibco, Waltham, Massachusetts) and 1X Penicillin-Streptomycin-Glutamine (Gibco# 10378016). Other reagents used in this study were Propidium Iodide (Invitrogen # P1304MP), Matrigel (BD Biosciences # 356237), Harris Hematoxylin (Leica # 3801560), Bluing Reagent (Thermo Scientific #7301), Clarifier 1 (Thermo Scientific #7401). ABC-HRP Kit (PK6100), and DAB Substrate Kit (SK-4100) were purchased from Vector Laboratories.
6
Mammary gland wholemount staining
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Wholemount staining was performed on the mammary glands of virgin mice using Harris's modified hematoxylin. Paraffin-embedded sections were used to perform immunohistochemical analyses. Ki-67 antibody (Ab15580) was from Abcam (Cambridge, MA, USA). F4/80 antibody (Q61549) was from Serotec (Oxford, United Kingdom). CD31 antibody (DIA-310) was from HistoBioTec (Miami beach, FL, USA). Stat3 (#9139), phospho-Stat3 (Tyr705, #9145), and beta-tubulin (#2128) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Secondary goat anti-mouse and secondary goat anti-rabbit antibodies were from Abcam. Positive signals were quantified using Image J software.
7
Paraffin-Embedded Tumor Tissue Analysis
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Tumor tissue was fastened with 4% paraformaldehyde (Solarbio) at room temperature for 20 min and encapsulated in paraffin to prepare paraffin sections. The sections were hatched overnight with diluted Ki67 antibody (ab15580, 1:100; Abcam) at 4°C, and hatched with the secondary antibody for 30 min. The samples were stained with diaminobenzidine (Solarbio) and observed under a microscope and photographed.
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