Kyse170

Manufactured by Japanese Collection of Research Bioresources Cell Bank

Sourced in Japan

KYSE170 is a cell line derived from human esophageal squamous cell carcinoma. It is maintained and distributed by the Japanese Collection of Research Bioresources Cell Bank as a research tool for studies related to esophageal cancer.

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Lab products found in correlation

Kyse70, by Japanese Collection of Research Bioresources Cell Bank (4 mentions) Rpmi 1640 medium, by Nacalai Tesque (4 mentions) Kyse150, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Horseradish peroxidase hrp conjugated anti rabbit or mouse secondary antibodies, by Cell Signaling Technology (2 mentions) Kyse30, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Phosphorylated p38, by Cell Signaling Technology (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Te 15, by RIKEN BioResource Center (1 mentions) Met 5a, by American Type Culture Collection (1 mentions) Het 1a, by American Type Culture Collection (1 mentions) Human umbilical vein endothelial cells (huvec), by PromoCell (1 mentions) Singlequot, by Lonza (1 mentions) Endothelial basal medium, by Lonza (1 mentions) Te 11, by RIKEN BioResource Center (1 mentions) Rabbit polyclonal antibodies against gapdh, by Santa Cruz Biotechnology (1 mentions) Rabbit monoclonal anti cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions) Rabbit monoclonal anti caspase 3 antibody, by Cell Signaling Technology (1 mentions) Kyse220, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Kyse510, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Kyse410, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)

8 protocols using kyse170

Esophageal Squamous Cell Carcinoma Cell Lines

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The human ESCC cell lines TE5, TE8, TE9, and TE15 were obtained from the Riken Cell Bank (Tsukuba, Japan). The human ESCC cell lines KYSE70, KYSE150, and KYSE170 were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). These cells were grown in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan) supplemented with 100 U/ml of penicillin, 100 μg/ml of streptomycin, and 10% fetal bovine serum (FBS). Cells were cultured in flasks or dishes in a humidified incubator at 37°C under 5% CO₂ in air.
The monoclonal anti-AE1 antibody used in the immunohistochemical analysis and protein assay was obtained from Abcam (Cambridge, MA, UK). The rabbit monoclonal c-Jun N-terminal kinase (JNK), phosphorylated JNK, extracellular signal-regulated kinase (ERK), phosphorylated ERK, p38, and phosphorylated p38 antibodies were purchased from Cell Signaling Technology (Beverly, MA). The mouse monoclonal ACTB antibody was purchased from Sigma-Aldrich (St. Louis, MO). Horseradish peroxidase (HRP)-conjugated anti-rabbit or mouse secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA).

Shiozaki A., Kudou M., Ichikawa D., Shimizu H., Arita T., Kosuga T., Konishi H., Komatsu S., Fujiwara H., Okamoto K., Kishimoto M., Marunaka Y, & Otsuji E. (2017). Expression and role of anion exchanger 1 in esophageal squamous cell carcinoma. Oncotarget, 8(11), 17921-17935.

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ESCC Cell Line Cultivation Protocol

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Human ESCC cell line TE13 was obtained from Riken Cell Bank (Tsukuba, Japan). The human ESCC cell line KYSE170 was obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). Cells were passaged and stored at −80°C in our laboratory for fewer than 6 months after receipt. All experiments were carried out within eight passages of resuscitation. The cells were maintained in a culture medium consisting of RPMI‐1640 (Life Technologies, Grand Island, NY, USA) supplemented with 10% heat‐inactivated FBS, 1 mM sodium pyruvate and 4 mM l‐glutamine at 37°C in 5% CO₂.

Ogino S., Konishi H., Ichikawa D., Matsubara D., Shoda K., Arita T., Kosuga T., Komatsu S., Shiozaki A., Okamoto K., Kishimoto M, & Otsuji E. (2018). Glutathione S‐transferase Pi 1 is a valuable predictor for cancer drug resistance in esophageal squamous cell carcinoma. Cancer Science, 110(2), 795-804.

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Esophageal Cell Lines for Research

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Human EC cell lines, KYSE170 and KYSE70, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). TE‐15, TE‐11, TE‐8, and TE‐2 were obtained from RIKEN Bioresource Center (Ibaraki, Japan). HUVEC was purchased from Promocell (Heidelberg, Germany). The human normal esophageal squamous epithelial cell line, HET‐1A, and human mesothelial cell line, MeT‐5A, were purchased from the ATCC (Rockville, MD, USA). These non‐tumor cell lines were used as the control. All EC cell lines, MeT‐5A, and HET‐1A, were maintained in RPMI medium (Nakalai Tisque), supplemented with 10%FBS (System Biosciences). HUVEC was cultured in an endothelial basal medium (Lonza) with endothelial growth supplement Single Quots (Lonza). All cell lines were cultured in an humidified 37°C incubator with 5% carbon dioxide.

Furuke H., Konishi H., Arita T., Kataoka S., Shibamoto J., Takabatake K., Takaki W., Shimizu H., Yamamoto Y., Komatsu S., Shiozaki A., Fujiwara H, & Otsuji E. (2022). Plasma microRNA‐192‐5p can predict the response to neoadjuvant chemotherapy and prognosis in esophageal cancer. Cancer Science, 114(4), 1686-1696.

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Culturing Esophageal and Biliary Cancer Cells

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Esophageal and biliary cancer were selected as the primary superficial cancers in our investigation. The human esophageal cancer cell lines KYSE30, KYSE70, and KYSE170 cells were obtained from the JCRB cell bank (Osaka, Japan). The KYSE30 cells were cultured in Dulbecco’s modified Eagle medium (D-MEM) supplemented with 2% fetal bovine serum (FBS). The KYSE70 cells were cultured in D-MEM supplemented with 5% FBS. The KYSE170 cells were cultured in the RPMI-1640/nutrient mixture F-12 medium supplemented with 2% FBS. The human cholangiocarcinoma cell lines HuCCT-1 and KKU-213 cells were obtained from the JCRB cell bank. The HuCCT-1 cells were cultured in RPMI-1640 medium supplemented with 10% FBS. The KKU-213 cells were cultured in the D-MEM supplemented with 10% FBS.
All cell culture media were supplemented with 2 mM L-glutamine solution without antibiotics. The cells were cultured in a humidified incubator with 5% CO₂ at 37 °C.

Nakada Y., Sugihara T., Tanaka M., Hamamoto W., Kanda T., Sakaguchi T., Kurumi H., Onoyama T., Ikebuchi Y., Takata T., Isomoto H, & Yamaguchi N. (2024). The Potential of Narrow-Band Imaging as a Novel Light Source for Photodynamic Therapy for Superficial Cancers via Endoscopes. Gastroenterology Research, 17(2), 72-81.

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Culturing and Characterizing ESCC Cell Lines

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The human ESCC cell lines TE2, TE5, TE9, and TE13 were obtained from the Riken Cell Bank (Tsukuba, Japan). The human ESCC cell line KYSE170 was obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). These cells were grown in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan) supplemented with 100 U/ml of penicillin, 100 μg/ml of streptomycin, and 10% fetal bovine serum (FBS). Cells were cultured in flasks or dishes in a humidified incubator at 37°C under 5% CO₂ in air.
The monoclonal anti-AE2 antibody used in the immunohistochemical analysis was obtained from Novus Biologicals (Littleton, CO). The monoclonal anti-AE2 antibody used in the protein assay was obtained from Santa Cruz Biotechnology (Dallas, TX). Rabbit polyclonal antibodies against GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Horseradish peroxidase (HRP)-conjugated anti-rabbit or mouse secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA).

Shiozaki A., Hikami S., Ichikawa D., Kosuga T., Shimizu H., Kudou M., Yamazato Y., Kobayashi T., Shoda K., Arita T., Konishi H., Komatsu S., Kubota T., Fujiwara H., Okamoto K., Kishimoto M., Konishi E., Marunaka Y, & Otsuji E. (2018). Anion exchanger 2 suppresses cellular movement and has prognostic significance in esophageal squamous cell carcinoma. Oncotarget, 9(40), 25993-26006.

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Cultivation of ESCC Cell Lines

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The human ESCC cell line TE5, TE8, TE19m and TE15 was obtained from the Cell Resource Centre for Biomedical Research at the Institute of Development, Aging, and Cancer (Tohoku University, Sendai, Japan). The human ESCC cell lineLYSE150 and KYSE170 was obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). The cells were cultivated using our previously reported protocols²⁰. These cell lines were grown in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan) supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum (FBS). Cells were cultured in flasks and dishes in a humidified incubator at 37 °C in 5% CO₂ in air. A rabbit monoclonal anti-TRPV2 antibody was used for the immunohistochemical analysis, and a protein assay was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The following antibodies were used in the Western blotting analysis: a rabbit monoclonal anti-caspase 3 antibody and rabbit monoclonal anti-cleaved caspase 3 antibody, which were purchased from Cell Signaling Technology (Beverly, MA). A mouse monoclonal anti-β-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Kudou M., Shiozaki A., Yamazato Y., Katsurahara K., Kosuga T., Shoda K., Arita T., Konishi H., Komatsu S., Kubota T., Fujiwara H., Okamoto K., Kishimoto M., Konishi E., Marunaka Y, & Otsuji E. (2019). The expression and role of TRPV2 in esophageal squamous cell carcinoma. Scientific Reports, 9, 16055.

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Esophageal and Neuroblastoma Cell Lines Cultivation

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Nine human ESCC cell lines, KYSE30, KYSE140, KYSE170, KYSE180, KYSE220, KYSE270, KYSE410, KYSE450, and KYSE510, were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank [22 (link)]. Two neuroblastoma cell lines, IMR-32 and KELLY, were obtained from the JCRB Cell Bank and Public Health England, respectively. KYSE140 was cultured in Ham's F12 medium containing 2% (v/v) FBS; KYSE30, KYSE170, KYSE180, KYSE220, KYSE270, KYSE410, KYSE450, and KYSE510 were cultured in Ham's F12/RPMI1640 medium containing 2% (v/v) FBS; IMR-32 was cultured in MEM medium containing 10% (v/v) FBS and non-essential amino acid (NEAA); and KELLY was cultured in RPMI1640 medium containing 10% (v/v) FBS.

Nakazato H., Takeshima H., Kishino T., Kubo E., Hattori N., Nakajima T., Yamashita S., Igaki H., Tachimori Y., Kuniyoshi Y, & Ushijima T. (2016). Early-Stage Induction of SWI/SNF Mutations during Esophageal Squamous Cell Carcinogenesis. PLoS ONE, 11(1), e0147372.

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Characterization of ESCC Cell Lines

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Cell Lines, Antibodies, and Other Reagents Seven human ESCC cell lines (TE2, TE4, TE5, TE8, TE9, TE13, and TE15) and the PK59 human pancreatic cell line were obtained from the Riken Cell Bank (Tsukuba, Japan), and four other human ESCC cell lines (KYSE70, KYSE150, KYSE170, and KYSE790) were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). Two cell lines (TE15, KYSE170) were used for in vitro studies, with these cells being cultured in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan) supplemented with 100 U/mL penicillin, 100 mg/mL streptomycin, and 10% fetal bovine serum under a humidified 5% CO 2 atmosphere at 37 C. 10e18 For immunohistochemistry, a polyclonal anti-LRRC8A antibody (ab157489) and a monoclonal anti-cytokeratin 13 antibody (ab16112) were purchased from Abcam (Cambridge, MA), whereas the monoclonal anti-LRRC8A antibody (SAB1412855) for the protein assay was from Sigma-Aldrich (St. Louis, MO), and the mouse monoclonal antibody that targeted b-actin was also from Sigma-Aldrich. Horseradish peroxidaseeconjugated anti-rabbit or mouse secondary antibodies were from Cell Signaling Technology (Beverly, MA).

Konishi T., Shiozaki A., Kosuga T., Kudou M., Shoda K., Arita T., Konishi H., Komatsu S., Kubota T., Fujiwara H., Okamoto K., Kishimoto M., Konishi E., Marunaka Y, & Otsuji E. (2019). LRRC8A Expression Influences Growth of Esophageal Squamous Cell Carcinoma. The American journal of pathology, 189(10).

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