The antibodies used in this study were anti-Fibronectin (rabbit polyclonal 1:400; Novusbio NBP1-91258), and conjugated FITC-CD31 (mouse monoclonal 1:100; Biolegend c.102405). The secondary antibody was Donkey anti-Rabbit Alexa-596 (1:200). Hematoxylin and eosin stain kit (Vectors Laboratory; H-3502).
Anti fibronectin
Manufactured by Novus Biologicals
Sourced in United States
Anti-Fibronectin is a laboratory reagent used for detecting and quantifying the protein fibronectin in biological samples. Fibronectin is an important extracellular matrix protein involved in various cellular processes. Anti-Fibronectin is a specific antibody that can be used in techniques such as Western blotting, ELISA, and immunohistochemistry to study the expression and distribution of fibronectin.
Automatically generated - may contain errors
Lab products found in correlation
Nbp1 91258, by Novus Biologicals (1 mentions)
Bovine serum albumin (bsa), by Dingguo (1 mentions)
Anti e cadherin, by Abcam (1 mentions)
Anti vimentin, by Abcam (1 mentions)
Anti macc1, by Abcam (1 mentions)
Goat anti rabbit igg, by Southern Biotech (1 mentions)
Trizol kit, by Takara Bio (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Dorsomorphin, by MedChemExpress (1 mentions)
Anti mmp2, by Novus Biologicals (1 mentions)
Anti mmp9, by Novus Biologicals (1 mentions)
Val boropro, by MedChemExpress (1 mentions)
Anti nlrc4, by Novus Biologicals (1 mentions)
Anti cd45, by Novus Biologicals (1 mentions)
Vx 765, by MedChemExpress (1 mentions)
Etoposide, by Apexbio (1 mentions)
Anti collagen type 1, by Proteintech (1 mentions)
Alexa fluor 488 conjugated secondary antibody, by Abcam (1 mentions)
Streptozocin, by Merck Group (1 mentions)
Anti β actin, by Proteintech (1 mentions)
3 protocols using anti fibronectin
1
Immunohistochemistry of Fibronectin and CD31
2
Antibody Procurement and Characterization
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Anti-MACC1, Anti-E-cadherin and anti-vimentin antibodies were obtained from Abcam (Cambridge, UK). Anti-MMP2, anti-p-AMPK, anti-MMP9 and anti-fibronectin antibodies were purchased from Novus Biologicals (Colorado, USA), Signalway Antibody (Maryland, USA) and Proteintech (Chicago, USA), respectively. Goat anti-rabbit IgG and rabbit anti-mouse IgG for western blotting were from SouthernBiotech (AL, USA). Alexa Fluor 448-labeled antibodies for immunofluorescence assays were from Beyotime (ShangHai, China). ACh was purchased from BioBasic Inc. (Toronto, Canada). Darifenacin and dorsomorphin were provided by MedChemExpress (NJ, USA). Lipofectamine 2000 reagent was from Invitrogen (CA, USA), while a Trizol kit was from Takara (Tokyo, Japan). Triton, bovine serum albumin (BSA) and DAPI were from DINGGUO CHANGSHENG (Beijing, China), Biosharp (AnHui, China) and Beyotime (ShangHai, China), respectively.
3
Antibody-based Protein Detection in Cellular Processes
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Anti‐GSDMD (Cat. no. NBP2‐33422), anti‐NLRC4 (Cat. no. NB100‐56142), anti‐CD45 (Cat. no. NB100‐77417) and anti‐fibronectin (Cat. no. NBP1‐91258) were obtained from Novus (Centennial, CO, USA). The anti‐caspase‐1 polyclonal antibody (Cat. no. ab1872) was obtained from Abcam (Cambridge, MA, USA). Anti‐IL‐1β (Cat. no. 12242) and anti‐caspase‐5 (Cat. no. 46680) were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti‐caspase‐4 (Cat. no. 11856‐1‐AP), anti‐collagen type I (Cat. no. 14695‐1‐AP) and anti‐β‐actin (Cat. no. 60008‐1‐Ig) were obtained from Proteintech (Wuhan, China). Alexa Fluor 488–conjugated secondary antibody (Cat. no. ab150089) was obtained from Abcam. VX‐765 (Cat. no. HY‐13205) and Val‐boroPro (Cat. no. HY‐13233A) were obtained from MedChemExpress (Monmouth Junction, NJ, USA). Etoposide (Cat. no. A1971) was obtained from ApexBio (Houston, TX, USA). Streptozocin (Cat. no. S0130) was obtained from Sigma Aldrich (St Louis, MO, USA).
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