Fei quanta 200 feg sem

Manufactured by Thermo Fisher Scientific

Sourced in United States

The FEI Quanta 200 FEG SEM is a scanning electron microscope that utilizes a field emission gun (FEG) as its electron source. It is capable of generating high-resolution images of sample surfaces at magnifications up to 1,000,000x.

Automatically generated - may contain errors

Lab products found in correlation

Invia raman microscope, by Renishaw (1 mentions) Cpd300 critical point dryer, by Leica (1 mentions) Pelco sc 6 sputter coater, by Ted Pella (1 mentions)

8 protocols using fei quanta 200 feg sem

Electrode Morphology and Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

To study the morphological features of the electrodes, we used a FEI Quanta 200 FEG SEM (FEI Company, Hillsboro, OR, USA) coupled to an EDX (X-ray energy dispersive system) for compositional analysis. SEM micrographs were usually taken working at 30 kV without any metallization of the samples.
Raman spectra were acquired by means of a Renishaw InVia Raman Microscope (Renishaw, Wotton-under-Edge, UK), equipped with a 532 nm Laser and focused on the sample with a Leica MSDS microscope (Leica Microsystems, Wetzlar, Germany) using a 50x long working distance magnification lens. Maximum laser power was equal to 140 mW, and it was reduced by means of Holographic filters ranging from 1 to 10% according to the sample’s response, with an accumulation time equal to 10 s and averaged for four accumulations for each spectrum.

Zaffora A., Megna B., Seminara B., Di Franco F, & Santamaria M. (2024). Ni,Fe,Co-LDH Coated Porous Transport Layers for Zero-Gap Alkaline Water Electrolyzers. Nanomaterials, 14(5), 407.

+ Open protocol

+ Expand

Characterizing LNP-F3 Nanoparticle Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols

The morphology of LNP-F3 was investigated using an FEI Quanta 200 FEG SEM (FEI Company, Hillsboro, OR, USA) operating with an accelerating electron voltage of 20 keV. Electron micrographs were acquired in a high-vacuum condition (3.8 × 10⁻⁵ mbar) and magnified up to 300,000×, setting the horizontal field and work distance at 853 nm and 10.7 mm, respectively. The surface electrical conductivity of the LNP-F3 was obtained by placing a few drops of fresh dispersion (diluted 1:1 with MilliQ water) onto an aluminium stub and placing it into a CaCl₂ desiccator at 4 °C for 24 h. Afterward, the dry sample was covered with a thin Au film deposited by sputtering before any investigation.

Angellotti G., Di Prima G., D’Agostino F., Peri E., Tricoli M.R., Belfiore E., Allegra M., Cancemi P, & De Caro V. (2023). Multicomponent Antibiofilm Lipid Nanoparticles as Novel Platform to Ameliorate Resveratrol Properties: Preliminary Outcomes on Fibroblast Proliferation and Migration. International Journal of Molecular Sciences, 24(9), 8382.

+ Open protocol

+ Expand

Plasma Clot Structural Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma clots from five patients (three Child score A and two Child score B) and five healthy individuals were generated and prepared for scanning electron microscopy as described [35] . Subjects were chosen based on clot permeability values closest to the median of their respective groups. Each clot was imaged in five different areas at 20 9 10 3 magnification using a FEI Quanta 200 FEG-SEM (FEI, Hillsboro, OR, USA). Average fiber diameters were measured from 50 random fibers in each sample using ImageJ software.

Hugenholtz G.C., Macrae F., Adelmeijer J., Dulfer S., Porte R.J., Lisman T, & Ariëns R.A. (2016). Procoagulant changes in fibrin clot structure in patients with cirrhosis are associated with oxidative modifications of fibrinogen. Journal of thrombosis and haemostasis : JTH, 14(5).

+ Open protocol

+ Expand

Characterization of PCL Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The morphology and structural features of PCL nanoparticles were examined by scanning electron microscopy (SEM) (FEI Quanta 200 FEG SEM, Thermo Fisher Scientific, Waltham, MA, USA). SEM specimens were prepared by dispersing the NP powder onto aluminium stubs (TAAB Laboratories, Berks, UK) with carbon-coated adhesive tabs, followed by sputter-coating with 10 nm gold for 2 min (Q150R coater, Quorum, Laughton, UK) to enhance conductivity.
For transmission electron microscopy (TEM), samples of liquid NP suspensions were dropped with a Pasteur pipette onto a carbon/formvar coated copper grid. After 15 s excess sample was blotted off with filter paper. Then a drop of stain (1% uranyl acetate) was added if required and blotted after 15 seconds. The grid was placed into a specimen holder and inserted into a Philips/FEI CM 120 BioTwin TEM (FEI Company, Hillsboro, OR, USA) for imaging at 120 kV.

Eleftheriadou D., Evans R.E., Atkinson E., Abdalla A., Gavins F.K., Boyd A.S., Williams G.R., Knowles J.C., Roberton V.H, & Phillips J.B. (2022). An alginate-based encapsulation system for delivery of therapeutic cells to the CNS. RSC Advances, 12(7), 4005-4015.

+ Open protocol

+ Expand

Scanning Electron Microscopy of Fish Spines

Check if the same lab product or an alternative is used in the 5 most similar protocols

One representative fish spine (≥4 IVDs) from each group (young wt and sp7^−/−) was processed for SEM. Fish were dehydrated with 99% ethanol and mechanically dissected, during which as much of the muscle and soft tissue surrounding the spine was removed as possible. Dissected spines were dehydrated using a Leica CPD300 Critical Point Dryer, mounted on SEM stubs and sputter coated with gold/palladium before being imaged in an FEI Quanta 200 FEGSEM (Thermo Fisher Scientific).

Kague E., Turci F., Newman E., Yang Y., Brown K.R., Aglan M.S., Otaify G.A., Temtamy S.A., Ruiz-Perez V.L., Cross S., Royall C.P., Witten P.E, & Hammond C.L. (2021). 3D assessment of intervertebral disc degeneration in zebrafish identifies changes in bone density that prime disc disease. Bone Research, 9, 39.

+ Open protocol

+ Expand

Exosome Characterization using SEM Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Exosomes captured on magnetic beads were fixed in 4% glutaraldehyde (in PBS) for 30min at RT. The beads were washed x3 in PBS and cytospun on epoxy silane coated glass slides (Nexterion, Schott AG). To characterize the beads, slides were air-dried overnight and gold-coated using a Pelco SC-6 sputter coater (Ted Pella, Inc., Redding, CA). Imaging was performed using an FEI Quanta 200 FEG SEM (Thermo Fisher Scientific, Waltham, MA) at Carnegie Mellon University Materials Characterization Facility.

Theodoraki M.N., Hong C.S., Donnenberg V.S., Donnenberg A.D, & Whiteside T.L. (2020). Evaluation of exosome proteins by on-bead flow cytometry. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 99(4), 372-381.

+ Open protocol

+ Expand

Scanning Electron Microscopy of Acalypha Plant

Check if the same lab product or an alternative is used in the 5 most similar protocols

The morphology structure of the tested plant was examined from the images established by SEM FEI Quanta 200 FEG (FEI Company, Hillsboro, OR, USA). The A. cordata plant parts powder samples were spread on an aluminum table and measured at three different locations. The samples were analysed in a low vacuum mode operating at 3.0 kV using an LDF detector.

Puzerytė V., Viškelis P., Balčiūnaitienė A., Štreimikytė P., Viškelis J, & Urbonavičienė D. (2022). Aralia cordata Thunb. as a Source of Bioactive Compounds: Phytochemical Composition and Antioxidant Activity. Plants, 11(13), 1704.

+ Open protocol

+ Expand

Oat Powder Morphological Structure Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The morphological structure of the tested plant was examined using the images obtained with SEM FEI Quanta 200 FEG (FEI Company, Hillsboro, OR, USA). The samples were analysed in low vacuum mode operating at 3.0 kV using an LDF detector. A. sativa powder samples from the control and enzymatically hydrolysed residue groups were spread on an aluminium table and measured at three different locations.

Streimikyte P., Kailiuviene J., Mazoniene E., Puzeryte V., Urbonaviciene D., Balciunaitiene A., Liapman T.D., Laureckas Z., Viskelis P, & Viskelis J. (2022). The Biochemical Alteration of Enzymatically Hydrolysed and Spontaneously Fermented Oat Flour and Its Impact on Pathogenic Bacteria. Foods, 11(14), 2055.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!