Salmonella shigella agar ssa

For each sample, 10 g were aseptically weighed in a sterile stomacher bag (Seward, London, UK), then homogenized in stomacher (Laboratory Blender, London, UK) with 90 ml of sterile peptone water 0.1 % (w/v) (Merck, Germany) for 2 min. Further decimal dilutions were prepared in 9 ml volumes of 0.1 % (w/v) peptone water and microbiological analyses were made using pour plate method (ICMSF, 1996) . Total aerobic bacterial count (TABC) on Plate Count Agar (PCA) (Merck, Germany), incubated at 30 °C for 48 h; Coliforms and E. coli most probable number "MPN" counts were done following the procedures described in AOAC (1995) . Staphylococci on Baird Parker Agar (BPA) (Merck, Germany) supplemented with Egg Yolk Tellurite Emulsion (Merck, Germany) incubated at 37°C for 2 d; yeast and mold on Rose Bengal Chloramphenicol agar (Merck, Germany) supplemented with Chloramphenicol Supplement (Merck, Germany) incubated at 25°C for 5 d; Psychrophilic bacterial count on Plate Count Agar (PCA) (Merck, Germany), incubated at 7 °C for 10 d and Salmonella on Salmonella Shigella agar (SSA) (Merck, Germany), Bacillus cereus was counted on selective agar base (Atlas, 2010) at 37°C for 72 h. Bacillus cereus grow as moderate-sized (5mm) colonies, which was turquoise, surrounded by a precipitate of egg yolk and turquoise (Ronald, 2010) .