DPPC, cholesterol, and 4-ATP were purchased from Fisher Scientific (Waltham, MA, USA). DOX was obtained from Cayman Chemicals (Ann Arbor, MI, USA). Tetrachloroauric acid trihydrate (HAuCl4·3H2O), AA, HA, and calcein AM/PI staining kit were purchased from Sigma–Aldrich (St Louis, MO). All chemicals were used as received without further treatment.
Calcein am pi staining kit
Manufactured by Merck Group
Sourced in United States
The Calcein AM/PI staining kit is a laboratory tool used for live/dead cell staining. It contains Calcein AM, a fluorescent dye that is absorbed by live cells, and Propidium Iodide (PI), a stain that enters only dead or damaged cells. This kit enables researchers to distinguish between viable and non-viable cells in a sample.
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Lab products found in correlation
2 protocols using calcein am pi staining kit
1
Liposomal Delivery of Doxorubicin
2
Cell Viability Assessment of Hydrogels
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Cell viability of the hydrogels toward mouse fibroblast (L929), human umbilical vein endothelial cell (HUVEC), RA synovial fibroblast (RA-FLS), and macrophage cell line RAW264.7 (104 cells per well) were evaluated by using a standard 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay (Solarbio, China). Cells were cocultured with Nap-GFFY, Nap-GDFDFDY, MTX-GFFY, MTX-GDFDFDY, and MTX with various concentrations (200, 100, 50, 10, 5, 0 μM), respectively, for 48 h. Then MTT assay was performed and the absorbance at 490 nm was measured using a microplate reader. Cell viability was also evaluated by Live/dead staining. After cocultured with the hydrogels or MTX (100 μM) for 48 h cells were stained with Calcein AM/PI staining kit (Sigma, USA) and then observed by a fluorescence microscope (Leica, Germany).
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