SIV ISH was performed on serial sections using next-generation ISH RNAscope, as previously described (32 (link)). The slides were scanned at high magnification (×400) using the Aperio AT2 digital Whole Slide Scanning System (Leica Biosystems), yielding high-resolution data from the entire tissue section. After regions of interest (ROIs) were determined and mapped on the digital Aperio scans of each tissue, laser capture microdissection was performed on the subjacent serial sections aligned to the specified ROIs using an ArcturusXT, Nikon TE2000 Microscope laser capture microdissection system. The targeted areas were captured on different caps (CapSure Macro LCM Caps, Applied Biosystems), as previously described (42 (link)). Each tube was inverted to allow total immersion of the tissue in the protease K solution and incubated at 65°C for 20 hours. The samples were then used for viral sequencing, as described above.
Aperio at2 digital whole slide scanning system
Manufactured by Leica
Sourced in Germany
The Aperio AT2 is a digital whole slide scanning system designed by Leica. It captures high-resolution digital images of microscope slides. The system is capable of scanning a variety of slide types and can create digital slide images for use in digital pathology workflows.
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Lab products found in correlation
2 protocols using aperio at2 digital whole slide scanning system
1
Spatial mapping of viral RNA by Next-Gen ISH
2
Thyroid Follicular Lesion Categorization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The NCC Ethics Committee/Institution Review Board (2021031709490902) approved our research. In this study, patient consent was not required as participants were not at risk. We collected 1,374 thyroid FSs in the Pathology Department of NCC/CICAMS from September 2018 to December 2020, which includes the following: 536 PTC, 72 thyroid adenomatous lesions (TAL), 45 thyroid fibrous calcified nodules (TFCN), 691 nodular goiters (NG), 5 other thyroid carcinomas (Other TC: which includes medullary thyroid carcinoma, undifferentiated carcinoma, and poorly differentiated carcinoma), and 25 other benign thyroid lesions (Other BTL: which includes thyroiditis and granulomatous thyroiditis) (Table 1 ). To generate the WSIs, all FSs were scanned using the Aperio AT2 Digital Whole Slide Scanning System (Leica Biosystems, Germany). Notably, we have excluded images containing artifacts of tissue processing, including air bubbles, folding, handwriting, crushing, poor staining, and blurring when scanning the sections, which seriously affected the recognition.
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