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Anti gabarapl1

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Anti-GABARAPL1 is a primary antibody that recognizes the GABARAPL1 (Gamma-aminobutyric acid receptor-associated protein-like 1) protein. GABARAPL1 is a member of the GABARAP protein family and is involved in the regulation of autophagy and intracellular trafficking.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Chloroquine, by Fujifilm (1 mentions) Anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Anti ha 12ca5, by Merck Group (1 mentions) Anti α tubulin dm1a, by Merck Group (1 mentions) Mg132, by Peptide Institute (1 mentions) Anti myc sc 40, by Santa Cruz Biotechnology (1 mentions) Anti nrf1 d5b10, by Cell Signaling Technology (1 mentions) Anti ulk1 d8h5, by Cell Signaling Technology (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Anti gapdh 6c5, by Santa Cruz Biotechnology (1 mentions) Chloroquine, by Merck Group (1 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti p β catenin, by Cell Signaling Technology (1 mentions) Anti p β catenin ser 552, by Cell Signaling Technology (1 mentions) Anti sqstm1 p62 ab101266, by Abcam (1 mentions) Anti vimentin, by Cell Signaling Technology (1 mentions) Z vad fmk, by Selleck Chemicals (1 mentions)

2 protocols using anti gabarapl1

1

Investigating Autophagy Regulation Mechanisms

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In this study, we utilized MG132 (Z-Leu-Leu-Leu-H (aldehyde), Peptide Institute), bafilomycin A1 (Sigma), and chloroquine (Wako). The antibodies utilized in this study were anti-NRF1 (D5B10; Cell Signaling Technology), anti-p62 (PM045; MBL), anti-S403-P-p62 (4F6; MBL), anti-ULK1 (D8H5; Cell Signaling Technology), anti-TBK1 (ab109735; Abcam), anti-GFP (sc-9996; Santa Cruz), anti-LC3B (L7543; Sigma), anti-GAPDH (6C5; Santa Cruz), anti-α-tubulin (DM1A; Sigma), anti-HA (12CA5; Sigma), and anti-Myc (sc-40; Santa Cruz) for immunoblot analyses; anti-p62 (PM066; MBL), anti-ULK1 (F-4; Santa Cruz), and anti-S172-P-TBK1 (D52C2; Cell Signaling Technology) for immunofluorescence analysis; anti-ubiquitin (clone FK2) (D058-3; MBL), anti-GABARAPL1 (D5R9Y; Cell Signaling Technology) for immunofluorescence and immunoblot analyses, anti-HA (3F10; Sigma) for immunoprecipitation; and normal rabbit anti-IgG antibody (Wako) and rabbit anti-Nrf1 polyclonal antibody (raised against mouse NRF1 residues from 292 to 741)33 (link) for ChIP assay.
Hatanaka A., Nakada S., Matsumoto G., Satoh K., Aketa I., Watanabe A., Hirakawa T., Tsujita T., Waku T, & Kobayashi A. (2023). The transcription factor NRF1 (NFE2L1) activates aggrephagy by inducing p62 and GABARAPL1 after proteasome inhibition to maintain proteostasis. Scientific Reports, 13, 14405.
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2

Isolation and Characterization of Dip G

Cited 1 time
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Dip G (>98% purity) was isolated as previously described22 (link). Chloroquine, oil red O, sodium butyrate (NaB), methyl thiazolyl tetrazolium (MTT) and tamoxifen (TAM) were purchased from Sigma-Aldrich (St. Louis, MO). Z-VAD-FMK and necrostatin-2 were from Selleck (Houston, TX). Anti-E-cadherin (14472), anti-vimentin (5741), anti-N-cadherin(13116), anti-p-β-catenin (Ser33/37/Thr41) (9561), anti-p-β-catenin (Thr41/Ser45) (9565), anti-p-β-catenin (Ser552) (5651), anti-p-β-catenin (Ser675) (4176), anti-β-catenin (8480), anti-c-Myc (18583), anti-LC3B (3868) and anti-GABARAPL1 (26632) were purchased from Cell Signaling Technology (Beverly, MA). Polyclonal anti-GABARAPL1 (11010-1-AP) was from Proteintech Group (Chicago, IL). Anti-ERα (sc-8002), anti-ERβ (sc-373853 X) and anti-β-Actin (sc-47778) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-SQSTM1/p62 (ab101266) was from Abcam (Cambridge, UK). CD24 monoclonal antibody (FITC) (11-0247-42), CD44 monoclonal antibody (PE-eFluor 610) (61-0441-80), Lipofectamine 3000 and Lipofectamine RNAi MAX were purchased from Thermo Fisher Scientific (Waltham, MA).
Fan M., Chen J., Gao J., Xue W., Wang Y., Li W., Zhou L., Li X., Jiang C., Sun Y., Wu X., Wu X., Ge H., Shen Y, & Xu Q. (2020). Triggering a switch from basal- to luminal-like breast cancer subtype by the small-molecule diptoindonesin G via induction of GABARAPL1. Cell Death & Disease, 11(8), 635.
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