Mouse cd31 clone mec 13.3

Manufactured by BD

Mouse CD31 (clone MEC 13.3) is a laboratory reagent used for the identification and quantification of mouse CD31 (also known as PECAM-1) by flow cytometry. CD31 is a cell surface glycoprotein that is expressed on endothelial cells, platelets, and some leukocytes. This reagent can be used to detect and analyze the presence of CD31-positive cells in mouse samples.

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Lab products found in correlation

Human ccl2, by R&D Systems (1 mentions) αsma a2547, by Merck Group (1 mentions) Ab14917, by Abcam (1 mentions)

Spelling variants of this product

Clone MEC 13.3 (23 citations) CD31 (clone MEC 13.3, (15 citations) CD31 (MEC 13.3, (9 citations)

2 protocols using mouse cd31 clone mec 13.3

Antibody Procurement for Immunohistochemistry

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Antibodies were purchased commercially as follows: mouse CD31 (clone MEC 13.3; BD Pharmingen, San Diego, CA), mouse F4/80 (clone BM8; eBioscience, San Diego, CA), human CD68 (clone PG‐M1; Dako, Glostrup, Denmark), human CCL2 (R&D Systems), mouse iNOS (#ab15323; abcam, Cambridge, MA), mouse arginase1 (#sc‐20150; Santa Cruz biotechnology, Santa Cruz, CA), and mouse MRC1/CD206 (#ab64693; abcam).

Arakaki R., Yamasaki T., Kanno T., Shibasaki N., Sakamoto H., Utsunomiya N., Sumiyoshi T., Shibuya S., Tsuruyama T., Nakamura E., Ogawa O, & Kamba T. (2016). CCL2 as a potential therapeutic target for clear cell renal cell carcinoma. Cancer Medicine, 5(10), 2920-2933.

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Immunohistochemical Analysis of Tumor Vasculature

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Tumor sections (5 μm cryostat sections) were fixed in 4% paraformaldehyde for 20 min at room temperature and blocked in 1% donkey serum in tris-buffered saline (TBS) for 2 h. Sections were then incubated overnight with anti-rabbit LYVE-1 polyclonal (Ab14917, 1:200; Abcam) or rat monoclonal anti-mouse CD31 (clone MEC 13.3, 1:1000; BD Pharmingen) and monoclonal anti- mouse α-smooth muscle actin (αSMA A2547, 1:1000; Sigma) antibodies. Preparations were mounted and analyzed with a Leica microscope, and counted at a 10x magnification.

Dumond A., Brachet E., Durivault J., Vial V., Puszko A.K., Lepelletier Y., Montemagno C., Pagnuzzi-Boncompagni M., Hermine O., Garbay C., Lagarde N., Montes M., Demange L., Grépin R, & Pagès G. (2021). Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 40, 33.

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