Biochemicals and analytical-grade inorganic chemicals were purchased from Fisher Scientific (Atlanta, GA), Bio-Rad (Hercules, CA) and Sigma-Aldrich (St. Louis, MO). Primers were ordered from Eurofins Genomics (Louisville, KY, USA) and used without further purification. Monarch PCR and DNA Cleanup Kit (5 μg), Phusion DNA polymerase, PCR reagents, and DpnI restriction enzyme were from New England Biolabs, while Hi-Lo DNA standards were from Minnesota Molecular, Inc. (Minneapolis, MN).
Pcr reagents
Manufactured by New England Biolabs
Sourced in United Kingdom, United States
PCR reagents are a collection of chemical compounds used in the Polymerase Chain Reaction (PCR) process. These reagents include DNA polymerase enzymes, DNA templates, nucleotides, and buffers that facilitate the amplification of specific DNA sequences. The core function of PCR reagents is to enable the exponential replication of DNA fragments for various applications, such as genetic analysis, diagnostic testing, and molecular biology research.
Automatically generated - may contain errors
Lab products found in correlation
Dpni restriction enzyme, by New England Biolabs (2 mentions)
Monarch pcr and dna cleanup kit 5 μg, by New England Biolabs (2 mentions)
Phusion dna polymerase, by New England Biolabs (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Dna extraction kit, by Qiagen (1 mentions)
Cd cho media, by Thermo Fisher Scientific (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Plasmid purification kit, by Qiagen (1 mentions)
Nitrocefin, by Thermo Fisher Scientific (1 mentions)
Human lysozyme, by Merck Group (1 mentions)
Anti mhc 2 dr antibody, by BioLegend (1 mentions)
Delfia eu labeled streptavidin, by PerkinElmer (1 mentions)
Microamp optical adhesive film, by Thermo Fisher Scientific (1 mentions)
Sypro orange 5000 protein stain, by Merck Group (1 mentions)
Microamp fast optical 0.1 ml 96 well plates, by Thermo Fisher Scientific (1 mentions)
Oligonucleotides for sequencing and, by Integrated DNA Technologies (1 mentions)
Growth media, by BD (1 mentions)
Peptides derived from p99βl, by GenScript (1 mentions)
Ni nta resin, by Qiagen (1 mentions)
Pcr cleanup and gel extraction kits, by Zymo Research (1 mentions)
7 protocols using pcr reagents
1
PCR Reagents and DNA Cleanup
2
Reagents and Materials Sourcing
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Chemicals were purchased from Sigma‐Aldrich (St. Louis, MO) and cell culture reagents were sourced from Gibco/ThermoFisher (Waltham, MA). CD‐CHO media was purchased from Thermo Fisher Scientific Inc. (Waltham, MA). DNA size markers, enzymes and PCR reagents were purchased from NEB (Ipswich, MA). DNA extraction kits were purchased from Qiagen (Germantown, MD). Oligonucleotide primers and probes were commercially synthesized by IDT (Coralville, IA).
3
Medaka Fish Husbandry Protocol
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Chemicals were purchased from Sigma, enzymes were from New England Biolabs, and PCR reagents were from TaKaRa unless otherwise indicated. Fish work was performed in strict compliance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Advisory Committee for Laboratory Animal Research in Singapore and approved by this committee (Permit Number: 27/09). Medaka strains HB32C, i3, HdrR and transgenic lines (see below) were maintained under an artificial photoperiod of 14-h light to 10-h darkness at 26 °C as described34 (link).
4
Oligonucleotides and Protein Purification Protocol
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oligonucleotides for sequencing and standard PCR methods (25 nmol scale, standard desalting) and oligonucleotides for gene synthesis (100 nmol scale, PAGE Purified) were purchased from Integrated DNA Technology (San Diego, CA). Nitrocefin was purchased from Oxoid (Cambridge, UK). Human lysozyme and SYPRO Orange 5000× Protein Stain were purchased from Sigma (St. Louis, MO). MicroAmp Fast Optical 0.1 ml 96-Well Plates and MicroAmp Optical Adhesive Film were from Applied Biosystems (Bedford, MA). Restriction enzymes and PCR reagents were purchased from New England BioLabs (Ipswich, MA). Growth media was purchased from Becton Dickinson (Franklin Lakes, NJ). Plasmid purification kits and Ni-NTA resin were purchased from Qiagen (Valencia, CA). PCR cleanup and gel extraction kits were from Zymo Research (Irvine, CA). Peptides derived from P99βL were ordered from GenScript (Piscataway, NJ), and were greater than 85% pure. Biotinylated tracer peptides were purchased from 21st Century Biochemicals (Marlborough, MA). MHC II DR molecules were purchased from Benaroya Research Institute (Seattle, WA), anti-MHC II-DR antibody from Biolegend (San Diego, CA), and DELFIA Eu-labeled Streptavidin was from PerkinElmer (Boston, MA). Unless noted, all other chemicals and reagents were from VWR (Radnor, PA).
5
Neuroprotective Effects of Oleuropein and Rutin
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PC12 cells were obtained from American Type Culture Collection (Manassas, VA, USA). Oleuropein and Rutin were purchased from AppliChem GmbH (Saxony-Anhalt, Germany). Fetal bovine serum (FBS), horse serum, bovine serum albumin (BSA), L-glutamine, penicillin, streptomycin, RPMI 1640 medium were purchased from HyClone (Logan, UT, USA). Mouse nerve growth factor 2.5S (2.5S mNGF) was obtained from Promega (Madison, WI, USA). Collagen Type IV from human placenta was purchased from Sigma-Aldrich (Steinheim, Germany). 6-hydroxydopamine was obtained from Tocris (Minneapolis, MN, USA). Annexin V-FITC/PI Assay Kit was obtained from eBioscience (San Diego, CA, USA). High Pure RNA Isolation Kit and High Fidelity cDNA Synthesis Kit were obtained from Roche Diagnostics (Mannheim, Germany). BCA™ Protein Assay Kit was obtained from Thermo Scientific (Waltham, MA, USA). PCR reagents were purchased from New England Biolabs (Ipswich, UK). Rabbit mono- or poly-clonal antibodies to PDI, CHOP, PERK, PDI, ATF-4, IRE1α, β-Actin were obtained from CST (Danvers, MA, USA). All other reagents were purchased from Sigma or Millipore unless otherwise noted.
6
PCR Reagents and DNA Cleanup
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Biochemicals and analytical-grade inorganic chemicals were purchased from Fisher Scientific (Atlanta, GA), Bio-Rad (Hercules, CA) and Sigma-Aldrich (St. Louis, MO). Primers were ordered from Eurofins Genomics (Louisville, KY, USA) and used without further purification. Monarch PCR and DNA Cleanup Kit (5 μg), Phusion DNA polymerase, PCR reagents, and DpnI restriction enzyme were from New England Biolabs, while Hi-Lo DNA standards were from Minnesota Molecular, Inc. (Minneapolis, MN).
7
Detailed Biochemical Reagent Sources
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If not otherwise indicated, all chemicals, substrates, and reagents were obtained from Carl Roth GmbH (Karlsruhe, Germany) or Sigma-Aldrich (St. Louis, MO, USA). Auxiliary enzymes were purchased from Sigma-Aldrich or Megazyme (Bray, Ireland). DNA polymerases, restriction endonucleases, T4 ligase, DNase I, and PCR reagents were bought from New England Biolabs (Ipswich, MA, USA) or Thermo Fisher Scientific Inc. (Waltham, MA, USA). F6P and S7P were obtained as disodium salt and barium salt from Biosynth Carbosynth (Staad, Switzerland; Compton, UK). R5P was purchased as barium salt from TCI (Zwijndrecht, Belgium). Oligonucleotides were synthesized by Eurofins Scientific (Luxemburg, Luxemburg).
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