Pig anti insulin antibody

Manufactured by Agilent Technologies

Sourced in United States

The Pig anti-insulin antibody is a laboratory reagent designed for research applications. It is a specific antibody raised against insulin, a hormone produced by the pancreas that regulates blood sugar levels. This antibody can be used to detect and measure insulin in biological samples, such as cell cultures or blood, as part of research studies.

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Lab products found in correlation

Vectashield mounting medium with dapi, by Vector Laboratories (2 mentions) Rabbit anti β catenin antibody, by Santa Cruz Biotechnology (1 mentions) Rabbit anti dnmt1 antibody, by Abcam (1 mentions) Rabbit anti sox2 antibody, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium with 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Mouse anti ki 67, by Abcam (1 mentions) Mouse anti dnmt1, by Abcam (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Rabbit anti ifnγ, by Abcam (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Rabbit anti tnf α, by Abcam (1 mentions)

Spelling variants of this product

Anti-insulin (46 citations) Insulin (34 citations) Guinea pig anti-insulin antibody (18 citations) Anti-insulin antibody (15 citations) Polyclonal guinea pig anti-insulin antibody (9 citations) Insulin antibody (6 citations) Guinea pig anti-swine insulin antibody (3 citations) Anti-TM (1 citations)

3 protocols using pig anti insulin antibody

Dual Immunofluorescence for DNMT1, Sox2, β-catenin

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For the detection of protein expression of DNMT1, Sox2, and β-catenin in human and animal tissues, 5 μm-thick paraffin or optimum cutting temperature (OCT) sections were stained by dual IF. The sections were incubated with primary antibodies (1:100 dilution of rabbit anti-DNMT1 antibody [Abcam, Cambridge, MA], 1:50 dilution of rabbit anti-Sox2 antibody [Santa Cruz Biotech, Santa Cruz, CA], 1:100 dilution of rabbit anti-β-catenin antibody [Santa Cruz Biotech, Santa Cruz, CA], 1:200 dilution of mouse anti-PTH antibody [Abcam, Cambridge, CA], and 1:500 dilution of pig anti-insulin antibody [Dako, Carpinteria. CA]) overnight at 4°C. Slides were then incubated with secondary antibodies (1:200 dilutions each of anti-mouse Alexa Fluor 647, anti-pig Alexa Fluor 647, and anti-rabbit Alexa Fluor 488; Invitrogen, Grand Island, NY, USA) for 45 minutes in the dark. The slides were then mounted in Vectashield mounting medium with 4′, 6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Burlingame, CA, USA). Images were taken using a fluorescence microscope with a camera.

Yuan Z., Claros C.S., Suzuki M., Maggi E.C., Kaner J.D., Kinstlinger N., Gorecka J., Quinn T.J., Geha R., Corn A., Pastoriza J., Jing Q., Adem A., Wu H., Alemu G., Du Y.C., Zheng D., Greally J.M, & Libutti S.K. (2016). Loss of MEN1 activates DNMT1 implicating DNA hypermethylation as a driver of MEN1 tumorigenesis. Oncotarget, 7(11), 12633-12650.

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Fluorescent Immunohistochemistry for Pancreatic Tissues

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IF staining was used to evaluate the expression levels of Dnmt1 and Ki67 in pancreatic tissues from treated mice. The sections were incubated overnight at 4 °C with primary antibodies: pig anti-insulin antibody (1:500 dilution; DAKO, CA, USA), mouse anti-Dnmt1 (1:200 dilution), and mouse anti-ki67 (1:1000 dilution) (Abcam, Cambridge, MA), respectively. After washing, the slides were incubated with goat anti-mouse Alexa Fluor 488 (Life Technologies, Grand Island, NY) and goat anti-guinea pig Alexa Fluor 647 (Life Technologies, Grand Island, NY) (both 1:200 dilutions) for 45 min in the dark. The slides were assembled in Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Images were obtained with an epifluorescence microscope with an attached camera.

Yuan Z., Gardiner J.C., Maggi E.C., Adem A., Zhang G., Lee S., Romanienko P., Du Y.C, & Libutti S.K. (2018). Tissue-specific induced DNA methyltransferase 1 (Dnmt1) in endocrine pancreas by RCAS-TVA-based somatic gene transfer system promotes β-cell proliferation. Cancer gene therapy, 26(3-4), 94-102.

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Quantification of IFN-γ and TNF-α in Pancreatic Tissues

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IF staining was used for the evaluation of the expression levels of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in pancreatic tissues from Men1 KO and WT mice. The sections were incubated overnight at 4°C with primary antibodies: pig anti-insulin antibody (1:500 dilution; DAKO, CA, USA) and rabbit anti-IFN-γ (1:100 dilution; Abcam, MA, USA), rabbit anti-TNF-α (1:500 dilution; Abcam, MA, USA) antibodies. After washing, the slides were incubated with goat anti-rabbit Alexa Fluor 488 and goat anti-guinea pig Alexa Fluor 647 (both 1:200 dilutions; Thermo Fisher Scientific, NY, USA) for 45 min in the dark. The slides were assembled in Vectashield mounting medium with DAPI (Vector Laboratories, CA, USA). Images were obtained with an Epifluorescence microscope with an attached camera. The quantification of IF staining was performed by counting cells that strongly expressed the target proteins in 4 randomly selected fields per slide under 10 and 20x magnification.

Yuan Z., Gardiner J.C., Maggi E.C., Huang S., Adem A., Li G., Lee S., Slegowski D., Exarchakis A., Howe J.R., Lattime E.C., Zang X, & Libutti S.K. (2021). B7 immune-checkpoints as targets for the treatment of neuroendocrine tumors. Endocrine-related cancer, 28(2), 135-149.

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