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Enzyme linked immunosorbent assay

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Enzyme-linked immunosorbent assay (ELISA) is a widely used analytical technique for the detection and quantification of specific proteins, antibodies, or other biomolecules in a sample. It utilizes the principle of antigen-antibody interaction and an enzyme-catalyzed color change to provide a measurable signal proportional to the concentration of the target analyte.

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Lab products found in correlation

Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions) Rpmi 1640, by Harvard Bioscience (1 mentions) Vacutainer, by BD (1 mentions) Carefusion, by BD (1 mentions) Col 2, by Abcam (1 mentions) Dylight 800 4x peg conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Polydatin, by Merck Group (1 mentions) P akt, by Abcam (1 mentions) Col 1, by Abcam (1 mentions) Recombinant rat il 1β, by Merck Group (1 mentions) Tnf α, by Abcam (1 mentions) Collagenase type 2, by Merck Group (1 mentions) P pi3k, by Abcam (1 mentions) Interleukin 6 (il 6), by Abcam (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Gapdh, by Abcam (1 mentions) Anti nlrp3 ag 20b 0014 c100, by Adipogen (1 mentions) Anti asc, by Santa Cruz Biotechnology (1 mentions) Anti caspase 1 p10, by Santa Cruz Biotechnology (1 mentions)

26 protocols using enzyme linked immunosorbent assay

1

Quantifying Extracellular Matrix and Cell Dynamics

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Enzyme-linked immunosorbent assays (Cusabio, College Park, MD, USA) were used to determine the levels of collagen type I and III in the culture supernatants of seven groups of HSCs and myofibroblasts. Cell cycle progression and proliferation were assayed using propidium iodide staining and the Cell Counting Kit 8 (Dojindo, Kumamoto, Japan), respectively, after serum starvation for 24 h.
Moreover, the adipogenic activity of the seven groups of HSCs and myofibroblasts were assayed based on the expression of adipogenic transcription factors (TFs; PPARγ, C/EBPα, and RXRα) and normalized using β-catenin levels.
Pan Q., Guo C.J., Xu Q.Y., Wang J.Z., Li H, & Fang C.H. (2020). miR-16 integrates signal pathways in myofibroblasts: determinant of cell fate necessary for fibrosis resolution. Cell Death & Disease, 11(8), 639.
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2

Metabolic Biomarker Assessment Protocol

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Body height, body mass, waist circumference, and blood pressure were determined
and body mass index (BMI) was calculated. Fasting venous blood was collected and
centrifuged at 1509.3 × g, and FBG, triglycerides (TG), total
cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density
lipoprotein cholesterol (LDL-C) levels in the supernatant were measured using an
AU5400 automatic biochemical analyzer (Hitachi Medical Corporation, Tokyo,
Japan). Fasting insulin (FINS) was measured by radioimmunoassay, and hemoglobin
A1c (HbA1c) was determined by colorimetry using appropriate kits (Cusabio,
Houston, TX, USA). Serum resistin, leptin, adiponectin, and FFAs were detected
by enzyme-linked immunosorbent assays (Cusabio). Homeostatic model assessment of
insulin resistance (HOMA-IR) was calculated as = FPG × FINS/22.5.
Wang L.K., Wang H., Wu X.L., Shi L., Yang R.M, & Wang Y.C. (2019). Relationships among resistin, adiponectin, and leptin and microvascular complications in patients with type 2 diabetes mellitus. The Journal of International Medical Research, 48(4), 0300060519870407.
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3

Gut Hormone Profiling in Mice

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Blood was collected from the iliac vein into an EDTA anticoagulation tube and centrifuged (2500 rpm for 20 min), and the supernatant was collected. Cholecystokinin (CCK), peptide YY (PYY), aglucagon-like peptide 1 (GLP-1), ghrelin (GHR), and leptin (LEP) were detected via enzyme-linked immuno-sorbent assays (Cusabio Biotech, CN) according to the manufacturer’s instructions. For the detection of the insulin concentration, mice were fasted for 12 h, and the insulin level was measured via a radioimmunoassay. HOMA-IR was calculated as follows: HOMA-IR = fasting insulin (mmol/L) × fasting glucose (mU/L)/22.5.
Zhang C., Li H., Yan Y., Zhang X, & Tu Z. (2022). 5:2 intermittent fasting tapers food intake in the refeeding state and ameliorates metabolic disturbances in mice exposed to olanzapine. Frontiers in Psychiatry, 13, 926251.
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4

Plasma RIP3 Measurement by ELISA

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We used a commercially available enzyme-linked immunosorbent assay (CUSABIO, Wuhan, China) to measure plasma RIP3 as previously described (18 (link)). Performing personnel were blind to each subject's AKI status. Plasma concentrations below the limit of detection (15.6 pg/mL) were set to 15.6 pg/mL for statistical analysis, and are reported with this value in the results.
Shashaty M.G., Reilly J.P., Sims C.A., Holena D.N., Qing D., Forker C.M., Hotz M.J., Meyer N.J., Lanken P.N., Feldman H.I., Christie J.D, & Mangalmurti N.S. (2016). Plasma levels of receptor interacting protein kinase-3 (RIP3), an essential mediator of necroptosis, are associated with acute kidney injury in critically ill trauma patients. Shock (Augusta, Ga.), 46(2), 139-143.
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5

Quantifying Galanin Levels in Gastric Cancer

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To determine levels of galanin, fasting venous blood samples were collected from
the gastric cancer patients at 2 days prior to the surgery and at 7 days after
the surgery; fasting venous blood samples were collected from the healthy
volunteers on the day that each volunteer was screened for inclusion in the
study. The blood samples (2 ml) were collected in prechilled EDTA tubes,
containing 100 µl aprotinin (1 µg/ml); within 30 min after collection, the tubes
were centrifuged for 15 min at 1000 × g at 4°C. Plasma was separated into vials
and stored at −80°C until measurement. One hundred microliters of plasma from
each assay point was used to analyze galanin content using an enzyme-linked
immunosorbent assay (CUSABIO, Inc., Wuhan, China). All measurements were
performed in duplicate, and the mean of the two measurements was used for
statistical analyses. According to the manufacturer’s specifications, the range
of the assay was 4.7–300 pg/ml, and the average sensitivity of the assay was
1.17 pg/ml. Intra-assay precision coefficient of variation was <8% and
inter-assay precision coefficient of variation was <10%.
Zhang L., Fang P., Chai C., Shao L., Mao H., Qiao D., Kong G., Dong X., Shi M., Zhang Z, & Bo P. (2019). Galanin expression is down-regulated in patients with gastric cancer. The Journal of International Medical Research, 47(3), 1241-1249.
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6

TGF-β1 Expression in Breast Cancer Cells

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TGF-β1 (RapidBio, Dallas, TX, USA) expression in the MDA-MB231, T47D, and MCF-7 human breast cancer cell lines was detected by enzyme-linked immunosorbent assay (CusaBio, Wuhan, People’s Republic of China). MCF-7, MDA-MB231, and T47D cell counts were obtained after 2 days. A total of 5×105 cells for each cell line were placed in six-well plates with 2 mL of culture solution per well. The culture supernatant was collected into a sterile tube after cell culture for 48 hours and centrifuged for 20 minutes. The supernatant was measured at an absorbance of 450 nm.
Zhao Y., Hu J., Li R., Song J., Kang Y., Liu S, & Zhang D. (2015). Enhanced NK cell adoptive antitumor effects against breast cancer in vitro via blockade of the transforming growth factor-β signaling pathway. OncoTargets and therapy, 8, 1553-1559.
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7

Serum Lipid and Insulin Analysis

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Serum were obtained by centrifugation of clotted blood collected from the eye sockets of the mice and stored at -80 °C. The serum fasting insulin (FINS) levels were examined using an enzyme-linked immunosorbent assay (Cusabio, Wuhan, Hubei, China). The serum FFAs, triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) levels were examined using commercial reagent kits (Jiancheng, Nanjing, Jiangsu, China).
Li X., Wu Y., Zhao J., Wang H., Tan J., Yang M., Li Y., Deng S., Gao S., Li H., Yang Z., Yang F., Ma J., Cheng J, & Cai W. (2020). Distinct cardiac energy metabolism and oxidative stress adaptations between obese and non-obese type 2 diabetes mellitus. Theranostics, 10(6), 2675-2695.
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8

Quantification of SDF-1 and CXCR4 in Tissues

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Liver samples are weighed and immediately placed in 10 volumes (wt/vol) of a protease inhibitor cocktail containing 10 nmol/l EDTA, 2 mmol/l PMSF, 0.1 mg/ml soybean trypsin inhibitor, 1.0 mg/ml bovine serum albumin, and 0.002% sodium azide in isotonic PBS, pH 7.0. Tissues are disrupted with a tissue homogenizer, and lysates are incubated at 4 °C for 2 h. Samples are clarified by two rounds of centrifugation at 12,500 g for 10 min at 4 °C. SDF-1 concentrations in blood and liver are assessed by enzyme-linked immunosorbent assay (CUSABIO, TX, USA). CXCR4 concentration in human MSCs are assessed by ELISA (CUSABIO, TX, USA).
Jin W., Liang X., Brooks A., Futrega K., Liu X., Doran M.R., Simpson M.J., Roberts M.S, & Wang H. (2018). Modelling of the SDF-1/CXCR4 regulated in vivo homing of therapeutic mesenchymal stem/stromal cells in mice. PeerJ, 6, e6072.
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9

Cytokine Production in Ti-Treated Cells

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Cells were cultured with Ti(IV) ions (5, 25, 50, or 100 μM) alone or in combination with 5 μg/ml of PHA for 24 h. The cell supernatants were then collected and assayed for IL-1β, TNF-α, and RANKL concentrations by the enzyme-linked immunosorbent assay (Cusabio Biotech, China).
Chen J., Li Q., Pang Z., Gong M, & Tang L. (2018). Titanium Ions Promote Exogenous Calcium-Dependent Calcium Influx in Activated Jurkat T Cells: A Possible Mechanism to Explain Its Immunostimulatory Properties. Mediators of Inflammation, 2018, 3286905.
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10

Quantifying Inflammatory Cytokines in Pleural Tissue

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The presence of interferon-γ, IL-2, TNF-α, monocyte chemotactic protein (MCP) -1, and IL-6 in pleural tissue was quantified using an enzyme-linked immunosorbent assay (Cusabio, Wuhan, China).
Kim Y., Park S.Y., Jung H., Noh Y.S., Lee J.J, & Hong J.Y. (2019). Inhibition of NADPH Oxidase 4 (NOX4) Signaling Attenuates Tuberculous Pleural Fibrosis. Journal of Clinical Medicine, 8(1), 116.
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