Cefaclor

To prepare the antibiotics-induced depression model, mice were allocated randomly in each group (n = 6/group). Mice were administrated orally with Cefaclor (200 mg/kg/day, dissolved in 0.2 mL saline) once a day for 5 days. Control groups were treated with vehicle (0.2 mL saline) instead of Cefaclor. The doses of Cefaclor used in the study were established according to their clinical doses⁶⁵ and equivalent dose calculation based on body surface area^{66 (link)}. Cefaclor (PHR1283) was purchased from Sigma-Aldrich (St. Louis, MO).
To examine the antidepressant effects of fluoxetine on Cefaclor-induced anxiety- and depression-like behaviors in mice, mice were allocated randomly to each group (n = 8/group). fluoxetine (20 mg/kg/day, dissolved in 0.2 mL saline) was orally administrated once a day for 5 days^{67 (link)}. Control groups was treated with vehicle (0.2 mL saline) instead of fluoxetine. fluoxetine (PHR1394) was purchased from Sigma-Aldrich.

Dithiothreitol (DTT), glutathione (GSH), 2-mercaptoethanol, β-NADPH, 5,5-dithio-bis-(2-nitrobenzoic acid), ethanothiol, triethanolamine, 2-vinylpyridine, sulfosalicylic acid, bovine serum albumin (BSA), buthionine sulfoximine (BSO), tris(2-carboxyethyl)phosphine (TCEP), N-acetyl-L-cysteine, L-cysteine, cefaclor, and cephadroxil were products of Sigma. HSA, was from Sigma or purified from serum as described below. glutathione reductase (GSR) and Triton X-100 were purchased from Roche and Calbiochem, respectively. Microcon cells and Immobilon-P Transfer Membrane were obtained from Amicon and Millipore, respectively. The bicinchoninic acid (BCA) kit for protein determination was from Pierce. Amoxicillin (Clamoxil) was from Beecham. Ampicillin (AMP), benzylpenicillin (BP), and cefuroxime were from Laboratorios Normon (Madrid, Spain). Deuterium oxide (D₂O) for proton-nuclear magnetic resonance (¹H-NMR) studies was from VWR. ECL was purchased from GE Healthcare.