Cells were washed in PBS, fixed for 5 min with 95% methanol and permeabilized for 30 min at 4 °C with blocking buffer (3% BSA and 0.1% saponin in PBS). Then incubated with primary antibodies followed by secondary antibodies 1 h each, with intermittent washing. Finally, cells were incubated in 4′,6-diamidino-2-phenylindole (Dapi) and confocal images and Z-stacks were obtained using the Axio Observer.Z1 microscope with alpha Plan-Apochromat 63x/Oil DIC (UV) M27 (Zeiss) using Axiocam MR R3. (See Supplemental Materials and Methods for detail) For antigen retrieval protocol and immunocytochemistry with MitoTracker red see Supplemental Materials and Methods ).
Alpha plan apochromat 63x oil dic uv m27
Manufactured by Zeiss
The Zeiss Alpha Plan-Apochromat 63x/Oil DIC (UV) M27 is a high-magnification objective lens designed for microscopy applications. It features a 63x magnification with an oil immersion design and differential interference contrast (DIC) capabilities, making it suitable for use with ultraviolet (UV) light sources.
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Lab products found in correlation
2 protocols using alpha plan apochromat 63x oil dic uv m27
1
Immunocytochemistry and Confocal Microscopy
2
Immunofluorescence Staining of Cells
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Cells were washed in PBS, fixed for 5 minutes with 95% methanol and permeabilized for 30 minutes at 4ºC with blocking buffer (3% BSA and 0.1% saponin in PBS). Then incubated with primary antibodies followed by secondary antibodies 1 hour each, with intermittent washing Finally, cells were incubated in 4′,6-diamidino-2-phenylindole (Dapi) and confocal images and Z-stacks were obtained using the Axio Observer.Z1 microscope with alpha Plan-Apochromat 63x/Oil DIC (UV) M27 (Zeiss) using Axiocam MR R3. (See supplemental Materials and Methods for detail).
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