Starter 3100

Manufactured by Ohaus

Sourced in United States

The Starter 3100 is a compact and reliable pH/mV meter designed for everyday laboratory use. It features an intuitive user interface, automatic buffer recognition, and temperature compensation to provide accurate and reliable pH measurements. The Starter 3100 is a versatile instrument suitable for a wide range of applications in the laboratory.

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Lab products found in correlation

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Spelling variants of this product

Starter 3100 pH meter (5 citations) Starter 3100 Bench pH-Meter (2 citations) Starter 3100-F (2 citations) Starter 3100 pH Bench (2 citations)

27 protocols using starter 3100

Microbial and Physicochemical Analysis of Milk

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Milk samples (25 ml) were diluted in buffered peptone saline (225 ml, 0.5% w/v; peptone; 0.85% w/v; NaCl), mixed in stomacher bag (Seward 400, England) for 2 min. To quantify the various microbial groups, Increased sensitivity to <1 CFU (colony-forming unit) per mL was achieved by spread plating 1 mL of the undiluted sample onto the agar media as well as the 1:10 dilutions to eliminate any inhibitory effect that may be present in the undiluted sample. Total plate count (TPC) was carried out on plate count agar (PCA), incubated at 32°C for 72 h (18 ). The coliforms were determined by the most probable number (MPN) method according to the US standard method (19 ). Staphylococcus aureus was enumerated on Baird Parker agar supplemented with egg yolk according to (20 (link)). Listeria monocytogenes were detected according to (21 ) while the Escherichia coli was examined with MacConkey agar followed by 24 h incubation at 37°C according to (22 ).
Lactose, protein, fats, and total solids contents (%) were evaluated using Near Infra-Red Multipurpose Analyzer (MPA), Bruker Optik Gmbh (Ettlingen, Germany) (23 (link)). The pH of the samples was determined using a digital pH meter (Starter 3100; Ohaus, New Jersey, USA), and the titratable acidity was determined in triplicate using the standard method ISO/TS 11,869:2,012 (IDF/RM 150:2,012) (3 (link)).

Mbye M., Mohamed H., Ramachandran T., Hamed F., AlHammadi A., Kamleh R, & Kamal-Eldin A. (2021). Effects of Pasteurization and High-Pressure Processing of Camel and Bovine Cheese Quality, and Proteolysis Contribution to Camel Cheese Softness. Frontiers in Nutrition, 8, 642846.

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Microalga Remediation of Contaminated Water

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Batch cultures of living microalga P. duplex AARLG060 were cultivated in natural contaminated wastewater obtained from the rehabilitated reservoir. The modified medium that had been optimized for low cost and high Mn remediation from the previous study was used. It consisted of NaNO₃ (0.09438 g/L), KH₂PO₄ (0.02606 g/L), CaHCO₃ (0.0159 g/L), and MgSO₄.7H₂O (0.0500 g/L) (Thongpitak et al., 2018 ). Each treatment was grown in triplicate at ambient temperatures using LED illumination (111.81 μE m^–2 s^–1). The light conditions were set to simulate high light intensity for large-scale outdoor remediation. Microalgal growth was determined daily by the measurement of optical density at 665 nm (OD₆₆₅) using a spectrophotometer (genesis 20, ThermoScientific Fisher, United States). The pH value was measured using a pH meter (starter3100, OHAUS, United States). Initial microalga optical density was recorded at 665 nm (OD₆₆₅) and adjusted to 0.2 for each treatment. Additionally, 300 mL of microalga was cultivated in 500 mL Erlenmeyer flasks. The cultures were manually shaken three times per day to confirm that all alga cells had been suspended. Manual agitation was employed to prevent dissolved oxygen from agitation, which could interfere with Mn oxidation in the microalgal photosynthesis.

Thongpitak J., Pekkoh J, & Pumas C. (2019). Remediation of Manganese-Contaminated Coal-Mine Water Using Bio-Sorption and Bio-Oxidation by the Microalga Pediastrum duplex (AARLG060): A Laboratory-Scale Feasibility Study. Frontiers in Microbiology, 10, 2605.

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Synthesis of Gadolinium-based Nanoparticles

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Gadolinium(III) nitrate hexahydrate, Gd(NO₃)₃∙6H₂O (pure for analysis, Khimreaktiv, Russia), citric acid (ACS reagent, ≥99.5%, Sigma-Aldrich), branched polyethyleneimine, PEI as 50% aqueous solution, (PEIX, where X = 0, 1, 2, 3 for Mw 60 kDa, 25 kDa, 1.3 kDa, and 0.8 kDa, respectively, Fluka), poly(diallyldimethylammonium chloride), PDDC as 35% aqueous solution (Sigma-Aldrich), sodium chloride (chemically pure, Khimreaktiv, Russia), sodium hydroxide, nitric and hydrochloride acids (pure for analysis, Khimreaktiv, Russia) were used. Ultra-purified water (18.2 MΩcm resistivity at 25 °C) was produced from Direct-Q 5 UV equipment (Millipore S.A.S. 67, 120 Molsheim, France). Experiments and measurements were conducted at 298 K. The temperature was maintained using a Haake DC10 (Thermo Electron) cryo thermostat. Polymer concentrations are expressed in their “monomeric” units relative to their respective molecular weights.
A “Starter 3100” (Ohaus) pH-meter was used for measuring acidity values of solutions, and was calibrated using standard buffers (pH 4.01, 7.00 and 9.00); pH metric titration was performed using an A-600 autotitrator (Kyoto) with carbonate free alkali.

Burilova E., Solodov A., Shayimova J., Zhuravleva J., Shurtakova D., Evtjugin V., Zhiltsova E., Zakharova L., Kashapov R, & Amirov R. (2021). Design of High-Relaxivity Polyelectrolyte Nanocapsules Based on Citrate Complexes of Gadolinium(III) of Unusual Composition. International Journal of Molecular Sciences, 22(21), 11590.

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Fermented Rice Bran Extracts Modulate Lactobacillus

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Various concentrations (3.125, 6.25, 12.5, 25 and 50 mg/mL) of fermented rice bran H7 (H7F) and G13 (G13F) extracts were cocultured with L. delbrueckii (2 × 10⁴ cfu/mL) in 27.6 g/L de Man–Rogaosa–Sharpe (MRS) broth under anaerobic conditions at 37 °C for 24 h. Bacterial numbers were analyzed by flow cytometric analysis (BD FACSCantoTM II flow cytometer, BD Biosciences, San Jose, CA, USA) employing counting beads calculation (BD Biosciences, CA, USA) followed with pH value measurement, compared to nonfermented rice bran (H7NF and G13NF) extracts, by using pH meter (Starter 3100, Ohaus, Parsippany, NJ, USA).

Sutthanut K., Tippayawat P., Srijampa S., Phoksawat W., Vachirodom P, & Wandee R. (2022). Prebiotic, Antipathogenic Bacteria and Hypocholesterolemia Properties of Fermented Rice Bran Extracts Derived from Black Rice and Germinated Brown Rice. Foods, 11(22), 3704.

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Adsorption Optimization at Varied pH

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Adsorption was performed at various pH values that ranged from 3 to 10 at room temperature. The desired pH was adjusted with 0.01 M HCl and 0.01 M NaOH using a pH meter (OHAUS Starter 3100, Ohio, USA). The initial concentrations of the analytes were fixed at 10 mg L⁻¹ in 10 mL of an aqueous solution of the analytes with a sorbent dosage of 20 mg, which was agitated at 250 rpm for 120 min.

Md Yusoff M., Yahaya N., Md Saleh N, & Raoov M. (2018). A study on the removal of propyl, butyl, and benzyl parabens via newly synthesised ionic liquid loaded magnetically confined polymeric mesoporous adsorbent. RSC Advances, 8(45), 25617-25635.

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Comprehensive Characterization of BaTiO3 Nanoparticles

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A potentiostat (AMEL, models 2553 and 2700) with three electrode systems, i.e., a reference electrode (Ag/AgCl, 1 M saturated KCl), a platinum counter electrode, and high-quality pencil graphite, acting as a working electrode (WE) was employed to conduct the electrochemical tests. All three electrodes were properly washed by using double-distilled water (DDW) before each and every experiment. A digital pH meter (STARTER 3100, OHAUS) was used to adjust the desired pH of the solution. K. Roy's smart electronic balance (model number 108) and Abron Exports' magnetic stirrer hot plate (model number MSWHP) were used for weighing and stirring, respectively, during the synthesis of BaTiO₃ nanoparticles. For the characterization study, the following techniques were used: UV-visible (Specord 200+ of Analytik Jena's, serial no. 223E2003C, Germany), Fourier transform infrared (FTIR) (Shimadzu FTIR-8400S Spectrum), which performed in the range of 4000 to 500 cm⁻¹ at ambient temperature (25 ± 1 °C) using KBr pellets mixed at a ratio of 1 : 20, scanning electron microscopy (SEM) coupled with dispersive X-ray spectroscopy (EDX) (model JSM5910, Japan), Brunauer–Emmett–Teller (BET) analysis (Quantachrome-ASIQwin-USA), zeta potential (Nano Zs90) (Malvern, UK), X-ray diffraction (XRD) (JDX-3532, JEOL, Japan) (2θ range from 5 to 80°), and Raman analysis (InVia Raman microscope by RENISHAW UK).

Draz M.U., Zia Ul Haq M., Hayat A, & Ajab H. (2023). An ALP enzyme-based electrochemical biosensor coated with signal-amplifying BaTiO3 nanoparticles for the detection of an antiviral drug in human blood serum. Nanoscale Advances, 6(2), 534-547.

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Comprehensive Sausage Quality Analysis

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Moisture content (oven-drying method at 105℃), protein content (Kjeldahl
method), fat content (Soxhlet method), and ash content (muffle furnace method)
of the sausage samples were determined according to AOAC (2005) . The changes in pH values were monitored
directly using a digital pH meter (Starter-3100, Ohaus, NJ, USA). HygroLab-C1
(Rotronic, NY, USA) was used to measure the water activity (A_w) of
the sausage samples.

Ayyash M., Olaimat A., Al-Nabulsi A, & Liu S.Q. (2020). Bioactive Properties of Novel Probiotic Lactococcus lactis Fermented Camel Sausages: Cytotoxicity, Angiotensin Converting Enzyme Inhibition, Antioxidant Capacity, and Antidiabetic Activity. Food Science of Animal Resources, 40(2), 155-171.

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Sperm Motility Activation: Temperature, pH, and Hypoxia

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For activation of sperm motility, the following treatments were tested: temperature (29, 31, 33, and 35°C), pH (4 and 8), and interaction between pH and hypoxia (1 mgO₂·L⁻¹) in controlled activator solution (distilled water-0 mOsm kg⁻¹). The different temperatures were maintained using thermostats HOPAR model (Aquarium Heater H-606). The pHs 4 and 8 were achieved by adding HCl 1% and NaOH 1%, respectively. The pH values were then monitored using a digital pH meter (Ohaus, Starter 3100). Hypoxia was adjusted with the addition of gaseous nitrogen until the concentration of 1 mgO₂ L⁻¹. The sperm were activated in the proportion 2:20 (v:v), in a closed environment with controlled temperature (23°C). Immediately after activation, sperm were observed under an optical microscope (Leica DM500; 40x), by the same observer to avoid the subject bias of the analyzes. The motility time was monitored with the aid of a timer (s) being evaluated from the start of movement until 100% sperm immobility. To identify the percent of sperm motility, a scale from 0 to 100% was used, according to Cosson et al. (2008) (link), where: 1 = 0–5%, 2 = 5–25%, 3 = 25–50%, 4 = 50–75%, 5 = 75–100%. For each treatment, nine replicates were performed in triplicate.

Castro J.S., Braz-Mota S., Campos D.F., Souza S.S, & Val A.L. (2020). High Temperature, pH, and Hypoxia Cause Oxidative Stress and Impair the Spermatic Performance of the Amazon Fish Colossoma macropomum. Frontiers in Physiology, 11, 772.

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Broccoli Juice Fermentation with P. pentosaceus

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Broccoli (Brassica oleracea var. italica) used in this experiment was purchased from a local market in Jeonju, Korea. Broccoli juice was prepared as described by Xu et al. [38 (link)] with several modifications. After washing the broccoli with distilled water, the leaves, and stalks were removed, and the florets were homogenized in sterile distilled water at a ratio of 1:2 (broccoli/distilled water, g/mL). The prepared broccoli juice was filtered through a cloth to separate the solid components, transferred to an Erlenmeyer flask, and pasteurized at 60 °C for 5 min. P. pentosaceus JBCC 106 was pre-cultured in MRS broth medium at 37 °C for 24 h and inoculated into the broccoli juice and fermented at 37 °C for 36 h. During fermentation, 30 mL samples were collected at 0, 3, 6, 12, 24, and 36 h and stored at −20 °C until analysis. The pH of the stored samples was determined using a pH meter (Starter3100, Ohaus, Parsippany, NS, USA) and viable cell count was measured by dispensing each sample diluted in sterilized distilled water onto an MRS agar plate and incubating at 37 °C for 24 h. All experiments were conducted in triplicate.

Park S.K., Jin H., Song N.E, & Baik S.H. (2023). Probiotic Properties of Pediococcus pentosaceus JBCC 106 and Its Lactic Acid Fermentation on Broccoli Juice. Microorganisms, 11(8), 1920.

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Bioremediation of Crude Oil Contaminated Soil

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The pH of the soil samples (prepared by mixing 1 g soil sample in 2.5 mL of 0.01 M CaCl₂) was measured using a pH meter (Ohaus, starter 3100, Parsippany, NJ, USA) every week throughout the 4-week bioremediation.
Crude oil degradation in the soil treatments was analyzed using thin-layer chromatography-flame ionization detection (TLC-FID (IATROSCAN, MK-6s)). The analysis was performed by the Microbial Technology Service Center, Chulalongkorn University, Thailand, according to the method described by Maruyama and colleagues [23 (link)].

Mukjang N., Chitov T., Mhuantong W., Champreda V., Pathom-aree W., Sattayawat P, & Bovonsombut S. (2022). Bacterial Communities Associated with Crude Oil Bioremediation through Composting Approaches with Indigenous Bacterial Isolate. Life, 12(11), 1712.

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