Immunohistochemical staining was performed using a Ventana Discovery Ultra instrument (Roche, Catalog No. 05987750001). Paraffin-embedded sections were dewaxed, subjected to either citrate buffer [pH 6.0] or Tris EDTA [pH 8.0] for heat-induced antigen retrieval, and incubated for 40 min with the appropriate primary antibody to c-Fos (1:50 dilution, Santa Cruz Biotechnology, Catalog No. sc-166940), c-Myc (1:50 dilution, Abcam, Catalog No. ab32072), YAP (1:200 dilution, Novus Biologicals, Catalog No. NB110-58358), Cyclin D1 (1:200 dilution, Roche, Catalog No. 790-4508), keratin 14 (1:300 dilution, Biolegend, Catalog No. 905301), or involucrin (1:100 dilution, Sigma-Aldrich, Catalog No. I9018). For standard staining, the Discovery OmniMap anti-Rb HRP detection system (Roche, Catalog No. 760-4311) was used for detection and hematoxylin for counterstaining as indicated by the manufacturer. Quantitation of immunohistochemical signals were done using the ImageJ software by adjusting the signal threshold to the staining intensity and measuring the area of the resulting particles.
Cyclin d1
Manufactured by Roche
Cyclin D1 is a cell cycle regulatory protein that plays a crucial role in the progression of cells from the G1 phase to the S phase of the cell cycle. It is a key component of the cell cycle machinery and is essential for the normal division and proliferation of cells.
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Lab products found in correlation
3 protocols using cyclin d1
1
Immunohistochemical Staining and Quantification
2
Immunohistochemical Analysis of FFPE Tissues
3
Immunohistochemical Analysis of FFPE Tissues
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Routine sections of formalin-fixed paraffin-embedded (FFPE) tissue from the first left neck needle-core biopsy, the subsequent left neck excisional biopsy, the thyroidectomy and the thymus biopsy were stained with Hematoxylin and Eosin (H&E) and microscopically examined. Immunohistochemical (IHC) stains were performed and included CD79a (Roche), CD3 (Ventana), CD68 (Roche), CD1a (Roche), Langerin/CD207 (Leica), CD163 (Roche), BRAF VE1 (Ventana), Cyclin D1 (Roche) and Ki67 (Dako). The BRAF VE1 antibody clone is specific for the BRAF V600E mutant protein [33 (link)].
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