Mouse monoclonal anti γ tubulin antibody

Rabbit polyclonal anti-HDAC8 antibody were purchased from Abcam (Cambridge, MA, USA; Cat#: ab187139); mouse monoclonal anti-α-tubulin-FITC antibody, mouse monoclonal anti-acetyl-α-tubulin (Lys-40) antibody and mouse monoclonal anti-γ-tubulin antibody were purchased from Sigma (St. Louis, MO, USA; Cat#: F2168, T7451 and T6557); human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, USA; Cat#: CA95617); FITC-conjugated goat anti-rabbit IgG (H + L), TRITC-conjugated goat anti-rabbit IgG (H + L) and FITC-conjugated goat anti-mouse IgG (H + L) were purchased from Zhongshan Golden Bridge Biotechnology Co., LTD (Beijing, China).

Rabbit polyclonal anti-Kif2a antibody used in Western blot and immunofluorescence was purchased from Novus Biologicals (Littleton,CO); Mouse monoclonal anti-α-tubulin-FITC antibody and mouse monoclonal anti-γ-tubulin antibody were obtained from Sigma-Aldrich Co. (Cat# F2168, Ca#T6557); Rabbit polyclonal anti-bub3 antibody was obtained from Santa Cruz Biotechology (Ca#sc-28258); Human polyclonal anti-centromere antibody (ACA) was obtained from Antibodies Incorporated (Item # 15-234-0001). Alexa Fluor@ 488-conjugate Goat anti-Rabbit IgG (H + L) and Alexa Fluor @594-conjugate Goat anti-Rabbit IgG (H + L) were produced by Thermo Fisher Scientific (Catalog# A-11008, Catalog# A-11012); Cy5-conjugated goat anti-human IgG and Cy5-conjugated goat anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA) (Cat#115-175-146, Cat#115-175-062). Mouse monoclonal anti-β-actin antibody was purchased from Santa Cruz Biotechnology (SC-8432, Santa Cruz, CA).
All other reagents were purchased from Sigma Aldrich except when mentioned otherwise.

A rabbit polyclonal anti-Axin-1 antibody was purchased from Millipore (Billerica, MA, USA). Sheep polyclonal anti-BubR1 and rabbit polyclonal anti-Nek9 antibodies were from Abcam (Boston, USA). Mouse monoclonal anti-γ-tubulin antibody, anti-α-tubulin FITC-tagged antibody, and TRITC-tagged anti-phalloidin were obtained from Sigma-Aldrich (St Louis, USA). Alexa Fluor 546-conjugated donkey anti-rabbit and donkey anti-sheep antibodies were purchased from Life Technologies (Carlsbad, USA). An Alexa Fluor 546-conjugated donkey anti-mouse antibody was purchased from Invitrogen Life Technologies (Carlsbad, USA). Control rabbit IgG was purchased from Santa Cruz Biochemicals (Dallas, USA). Mounting medium containing DAPI was purchased from Vector Laboratories (Burlingame, USA) and milrinone was purchased from Cayman Chemical Co. (Ann Arbor, USA).