S bay k8644

([(3S)‐6‐({2′,6′‐dimethyl‐4′‐[3‐(methylsulfonyl)propoxy]biphenyl‐3‐yl}methoxy)‐2,3‐dihydro‐1‐benzofuran‐3‐yl]acetic acid hemihydrate) was synthesized at Takeda Pharmaceutical Company (Osaka, Japan). Diazoxide, KCl, and glimepiride were purchased from Wako Pure Chemical Industries (Osaka, Japan). Sterile D‐(+)‐glucose solution (45% in water), γ‐linolenic acid (γ‐LA), and (S)‐(‐)‐Bay K8644 were purchased from Sigma‐Aldrich (St. Louis, MO). D‐myo‐inositol 1,3,5‐trisphosphate hexakisacetoxymethyl ester, 2,4,6‐tri‐O‐butyryl (Bt₃IP₃), a cell‐permeable derivative of D‐myo‐inositol 1,3,5‐trisphosphate, was purchased from Merck Millipore (Tokyo, Japan). A cell‐permeable analog of DAG, 1‐oleoyl‐2‐acetyl‐sn‐glycerol (OAG), was purchased from Cayman Chemical (Ann Arbor, MI). Nifedipine was purchased from MP Biomedicals (Tokyo, Japan). Krebs‐Ringer‐bicarbonate‐HEPES (KRBH) buffer (116 mmol/L NaCl, 4.7 mmol/L KCl, 1.17 mmol/L KH₂PO₄, 1.17 mmol/L MgSO₄, 25 mmol/L NaHCO₃, 2.52 mmol/L CaCl₂, and 24 mmol/L HEPES (pH 7.4)) containing 0.2% bovine serum albumin (BSA) was freshly prepared before each experiment. Fura‐2 acetoxymethyl ester was purchased from Dojindo Laboratories (Kumamoto, Japan).