Chromatographic grade acetonitrile

Manufactured by Tedia

Sourced in United States

Chromatographic grade acetonitrile is a high-purity organic solvent commonly used as a mobile phase component in various chromatographic techniques, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC). It is a colorless, volatile liquid with a characteristic odor. The chromatographic grade ensures consistent quality and low levels of impurities, making it suitable for sensitive analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using chromatographic grade acetonitrile

Extraction and Separation of Sage Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Organic solvents including ethanol, petroleum ether, ethyl acetate, methanol, n-butanol, and chloroform used for crude extract preparation and HSCCC separation were all of analytical grade (Damao Chemical Factory, Tianjin, China). Chromatographic grade acetonitrile (Tedia Company Inc, Fairfield, USA) was used for HPLC analysis. The water used was pretreated with a Super Pure Water system (TYP10S, Jinan Taipingma Environmental Protection Equipment Co., Ltd.). The plant material of S. officinalis used here was the same as that in our previous study,^{14,15 (link)} as well as the medicine origin.

Yu J., Sun X., Zhao L., Wang X, & Wang X. (2020). An efficient method to obtain anti-inflammatory phenolic derivatives from Scindapsus officinalis (Roxb.) Schott. by a high speed counter-current chromatography coupled with a recycling mode. RSC Advances, 10(19), 11132-11138.

+ Open protocol

+ Expand

Analytical method for 2,4-D detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

2,4-Dichlorophenoxyacetic acid (2,4-D) (> 96% purity) was obtained from the Nanjing Chang Feng Agrochemical Co., Ltd. (Nanjing, China). Chromatographic grade acetonitrile and formic acid were purchased from TEDIA Company, Inc., OH Fairfield, USA. Ammonium acetate (Tiancheng Chemical Co., Ltd., Shanghai, China). HPLC-grade water was prepared using a Milli-Q purification system (Millipore, Bedford, MA, USA). All other reagents used in this study were of analytical or HPLC grade.

Chen X., Zhang H., Wan Y., Chen X, & Li Y. (2018). Determination of 2,4-Dichlorophenoxyacetic acid (2,4-D) in rat serum for pharmacokinetic studies with a simple HPLC method. PLoS ONE, 13(1), e0191149.

+ Open protocol

+ Expand

Analytical Procedure for Pesticide Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

β-CY (99.7%) and 3-PBA (98%) were obtained from the National Standard Substances Center (Beijing, China) and Sigma-Aldrich Chemical Co. (Shanghai, China), respectively. Chromatographic-grade acetonitrile was purchased from Tedia Co. (Fairfield, OH., USA). Acetonitrile, ethyl alcohol, KH₂PO₄, K₂HPO_4, MgSO₄, NaCl, NaOH, Na₂SO₄, (NH₄)₂SO₄, phenol, catechol, and gallic acid were of analytical grade and procured from Kelong Chemical Co. (Chengdu, China).

Zhao J., Chi Y., Xu Y., Jia D, & Yao K. (2016). Co-Metabolic Degradation of β-Cypermethrin and 3-Phenoxybenzoic Acid by Co-Culture of Bacillus licheniformis B-1 and Aspergillus oryzae M-4. PLoS ONE, 11(11), e0166796.

+ Open protocol

+ Expand

Isolation and Characterization of Lactobacillus plantarum PA01

Check if the same lab product or an alternative is used in the 5 most similar protocols

De Man, Rogosa, and Sharpe (MRS) medium was purchased from Beijing Obosing Biotechnology Co., Ltd. (Beijing, China). Lactobacillus plantarum PA01 was isolated from pickle and registered by the China Common Microbial Collections Management Center (CGMCC No.15660). Standards for high-performance liquid chromatography (HPLC) (gallic acid, ferulic acid, catechins, chlorogenic acid, rutin, phloridzin, epicatechin, quercetin, caffeic acid, phloretin) were purchased from Solarbio (Beijing, China). Chromatographic grade acetonitrile (TEDIA, Fairfield, OH, USA) and formic acid (Kemiou, Tianjin, China) were obtained for analysis.

Ma J., Wang Y., Zhao M., Tong P., Lv L., Gao Z., Liu J, & Long F. (2023). High Hydrostatic Pressure Treatments Improved Properties of Fermentation of Apple Juice Accompanied by Higher Reserved Lactobacillus plantarum. Foods, 12(3), 441.

+ Open protocol

+ Expand

Camellia Pollen Tyrosinase Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analytical‐grade ethanol, petroleum ether, ethyl acetate, and n‐butanol were purchased from Sinopharm Chemical Reagent Corporation. Chromatographic grade acetonitrile was purchased from Tedia Company Inc. Diaion HP‐20 macroporous adsorption resin was purchased from Beijing Green Herbs Co., Ltd. Mushroom tyrosinase (E.C.1.14.18.1), L‐tyrosine, and standards of arbutin and p‐coumaric acid ethyl ester were purchased from Merck Life Science Co., Ltd. Camellia pollen was purchased from Beijing Tong Ren Tang Co., Ltd.

Li L., Cai Y., Sun X., Du X., Jiang Z., Ni H., Yang Y, & Chen F. (2020). Tyrosinase inhibition by p‐coumaric acid ethyl ester identified from camellia pollen. Food Science & Nutrition, 9(1), 389-400.

+ Open protocol

+ Expand

Rotenone-based Microemulsion Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rotenone standard (98.0%) was purchased from Aldrich, USA; Rotenone (95.0%) was produced by Fengshun Tangxi Jiaxing Welfare Chemical Co., Ltd. China; 6.0% rotenone microemulsion (ME) was purchased from Beijing Sanpu Baicao Green Plant Preparation Co., Ltd. Cetyltrimethylammonium bromide (CTAB, 99%), absolute ethyl alcohol, tetraethyl orthosilicate (TEOS) and concentrated ammonia (25%) were purchased from Sinopharm Chemical Reagent Co., Ltd. Chromatographic grade acetonitrile was purchased from Tedia, USA. Water was prepared using a Milli-Q ultrapure water purifier for all experimental procedures.

Xu W., Shen D., Chen X., Zhao M., Fan T., Wu Q., Meng Z, & Cui J. (2023). Rotenone nanoparticles based on mesoporous silica to improve the stability, translocation and insecticidal activity of rotenone. Environmental science and pollution research international, 30(48).

+ Open protocol

+ Expand

Mass Spectrometry Analysis of Dry Fraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

The dry fraction was dissolved in a mixture containing 50% (v/v) chromatographic grade acetonitrile (Tedia, Fairfield, OH, USA), 50% (v/v) deionized water, and 0.1% formic acid. The solution was infused directly into the electrospray ionisation (ESI) source at a flow rate of 100 μL.min -1 , with the assistance of a high performance liquid chromatograph (HPLC; Shimadzu, Tokyo, Japan). ESI (+)-MS and ESI (-)-MS were acquired using a hybrid high-resolution and high accuracy (5 μL/L) micro-time of flight (TOF) (Q-TOF) mass spectrometer (Bruker Scientific ® , Billerica, MA, USA) under the following conditions: capillary and cone voltage, + 3500 V and + 40 V, respectively; and de-solvation temperature, 180°C. A TOF control software (Bruker Scientific ® ) was used for data acquisition and processing. The data was collected in an m/z range of 70-800 and at a speed of 2 scans/second; a resolution of 50,000 (FWHM) was obtained at m/z 200. No important ions were observed below m/z 100 or above m/z 600; therefore, the ESI (+)-MS data at the m/z range 295-350 is shown.

de S Wisintainer G.G., Scola G., Moura S., Lemos T.L., Pessoa C., de Moraes M.O., Souza L.G., Roesch-Ely M, & Henriques J.A. (2015). O-naphthoquinone isolated from Capraria biflora L. induces selective cytotoxicity in tumor cell lines. Genetics and molecular research : GMR, 14(4).

+ Open protocol

+ Expand

ESI-MS Analysis of Plant Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The dries extracts were dissolved in a solution of 50% (v/v) chromatographic grade acetonitrile (Tedia, Fairfield, OH, USA), 50% (v/v) deionized water, and 0.1% formic acid. The solutions were infused directly individually into the ESI source by means of a syringe pump (Harvard Apparatus) at a flow rate of 50 μL min⁻¹. ESI(+)-MS and tandem ESI(+)-MS/MS were acquired using a hybrid high-resolution and high accuracy (5 μg/L) microTof (Q-TOF) mass spectrometer (Bruker Scientific) under the following conditions: capillary and cone voltages were set to +3500 V and +40 V, respectively, with a desolvation temperature of 100°C. For ESI(+)-MS/MS, the energy for the collision induced dissociations (CID) was optimized for each component. Diagnostic ions in different fractions were identified by the comparison of their ESI(+)-MS/MS dissociation patterns with compounds identified in previous studies. For data acquisition and processing, compass software (Bruker Scientific) was used. The data were collected in the m/z range of 70–800 at the speed of two scans per second, providing the resolution of 50,000 (FWHM) at m/z 200. No important ions were observed below m/z 180 or above m/z 650; therefore ESI(+)-MS data is shown in the m/z 180−650 range.

Begnini K.R., Moura de Leon P.M., Thurow H., Schultze E., Campos V.F., Martins Rodrigues F., Borsuk S., Dellagostin O.A., Savegnago L., Roesch-Ely M., Moura S., Padilha F.F., Collares T., Pêgas Henriques J.A, & Seixas F.K. (2014). Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 639856.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!