Ifnar b6.129s2 ifnar1tm1agt mmjax, by Jackson ImmunoResearch - Product details

1

Generation of Knockout Mouse Strains

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C57BL/6J, MOLF/Ei, and Ifnar^−/−(B6.129S2-Ifnar1tm1Agt/Mmjax) (Müller et al., 1994 (link)) mouse strains were obtained from The Jackson Laboratory. Ifnb^−/− mice (Deonarain et al., 2003 (link)) were a gift from Dr. S. Vogel (University of Maryland). Mlkl^−/− (Murphy et al., 2013 (link)) mice were generated by Dr. W. Alexander (Walter and Eliza Hall Institute) and were a gift from Dr. M. Kelliher (University of Massachusetts). Ifnb^−/−Mlkl^−/− double knockout mice were generated by crossing Ifnb^−/− and Mlkl^−/− mice to homozygosity. Similarly, RIP1 Ki Ifnb^−/− mice were generated by crossing Ifnb^−/− and Ripk1^K45A/K45A mice to homozygosity. Akt1^−/−, Akt3^−/− and wild-type littermate controls (Easton et al., 2005 (link); Mao et al., 2007 (link)) were a gift from Dr. P. Hinds (Tufts University). 4E-BP triple knockout mice were a gift from Dr. N. Sonenberg (McGill University). RIP1/3 kinase inactive mice (Ripk1^K45A/K45ARipk3^K51A/K51A, RIPKi) (Berger et al., 2014 (link); Mandal et al., 2014 (link)) were gifts from Dr. A. Degterev. All genetically modified mice were fully backcrossed to the C57BL/6J background. All mice were housed in a pathogen-free facility at the Tufts University School of Medicine and experiments were performed in accordance with regulations and approval of the Tufts University Institutional Animal Care and Use Committee.

Muendlein H.I., Sarhan J., Liu B.C., Connolly W.M., Schworer S.A., Smirnova I., Tang A.Y., Ilyukha V., Pietruska J., Tahmasebi S., Sonenberg N., Degterev A, & Poltorak A. (2020). Constitutive Interferon Attenuates RIPK1/3-Mediated Cytokine Translation. Cell reports, 30(3), 699-713.e4.

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2

Generation of Knockout Mouse Strains

Cited 2 times

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C57BL/6J, MOLF/Ei, and Ifnar^−/−(B6.129S2-Ifnar1tm1Agt/Mmjax) (Müller et al., 1994 (link)) mouse strains were obtained from The Jackson Laboratory. Ifnb^−/− mice (Deonarain et al., 2003 (link)) were a gift from Dr. S. Vogel (University of Maryland). Mlkl^−/− (Murphy et al., 2013 (link)) mice were generated by Dr. W. Alexander (Walter and Eliza Hall Institute) and were a gift from Dr. M. Kelliher (University of Massachusetts). Ifnb^−/−Mlkl^−/− double knockout mice were generated by crossing Ifnb^−/− and Mlkl^−/− mice to homozygosity. Similarly, RIP1 Ki Ifnb^−/− mice were generated by crossing Ifnb^−/− and Ripk1^K45A/K45A mice to homozygosity. Akt1^−/−, Akt3^−/− and wild-type littermate controls (Easton et al., 2005 (link); Mao et al., 2007 (link)) were a gift from Dr. P. Hinds (Tufts University). 4E-BP triple knockout mice were a gift from Dr. N. Sonenberg (McGill University). RIP1/3 kinase inactive mice (Ripk1^K45A/K45ARipk3^K51A/K51A, RIPKi) (Berger et al., 2014 (link); Mandal et al., 2014 (link)) were gifts from Dr. A. Degterev. All genetically modified mice were fully backcrossed to the C57BL/6J background. All mice were housed in a pathogen-free facility at the Tufts University School of Medicine and experiments were performed in accordance with regulations and approval of the Tufts University Institutional Animal Care and Use Committee.

Muendlein H.I., Sarhan J., Liu B.C., Connolly W.M., Schworer S.A., Smirnova I., Tang A.Y., Ilyukha V., Pietruska J., Tahmasebi S., Sonenberg N., Degterev A, & Poltorak A. (2020). Constitutive Interferon Attenuates RIPK1/3-Mediated Cytokine Translation. Cell reports, 30(3), 699-713.e4.

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Mouse Strains for Immunological Studies

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B10.A and B10.A.SJL-Rag2^−/− -Ptprc^a-5C.C7(TcRa, TcRb) were obtained from Taconic Farms (Germantown, NY). C57BL/6, C57BL/6 OT-II (B6.Cg- Tg (TcraTcrb) 425Cbn/J, Tram^−/− C57BL/6J- Ticam1Lps2/J, TLR3^−/− B6;129S1- Tlr3tm1Flv/J, MyD88^−/− CBy.129P2(B6) - MyD88tm1Defr/J, IFNAR^−/− B6.129S2- Ifnar1tm1Agt/Mmjax, IL-1R^−/− B6.129S7-Il1r1tm1Imx/J were purchased from Jackson Laboratories (Bar Harbor, ME). All mice were housed under specific pathogen-free animal conditions at the National institute of Allergy and Infectious Diseases (NIAID), and used between 6 and 12 weeks of age in accordance with guidelines provided by the Institutional Animal Care and Use Committee of the NIAID.

Caucheteux S.M., Hu-Li J., Mohammed R., Ager A, & Paul W.E. (2016). Cytokine Regulation of Lung Th17 Response to Airway Immunization using LPS Adjuvant. Mucosal immunology, 10(2), 361-372.

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Evaluation of Knockout Mice in Immune Responses

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All animal studies were approved by the University of Michigan Committee on Use and Care of Animals. BALB/c, C57BL/6, CD8α^-/- [B6.129S2-Cd8a^tm1Mak/J], IFN-γ^-/- [B6.129S7-Ifng^tm1Ts/J], and IFNAR^-/- [B6.129S2-Ifnar1^tm1Agt/Mmjax] mice (all knockouts backcrossed onto a C57BL/6 background) were obtained from the Jackson Laboratory (Bar Harbor, ME). Mice heterozygous for microsomal prostaglandin E synthase-1 (mPGES-1) on a DBA1lac/J background (mPGES-1^-/- mice) [42 (link)] were originally obtained from Pfizer, Inc. (Groton, CT) and then backcrossed onto a C57BL/6 background. Homozygous mPGES-1^-/- mice and homozygous wt mPGES-1^+/+ mice derived from the same heterozygous mPGES-1^+/- parents were bred at the University of Michigan. Adult males were used in all experiments. All mice were maintained under specific pathogen-free conditions.

McCarthy M.K., Procario M.C., Wilke C.A., Moore B.B, & Weinberg J.B. (2015). Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation. PLoS ONE, 10(9), e0139235.

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Controlled Microbiome in Genetically Modified Mice

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Mice used for these experiments were maintained on a C57BL/6 background. C57BL/6 (WT) and IFNAR^–/– (B6.129S2-Ifnar1tm1Agt/Mmjax) were purchased from Jackson Laboratories. RAG^–/–OT-II^+/–CD45.1^+/+ mice were provided by Peter Savage. Mice were maintained in specific pathogen-free (SPF) environments at the University of Pittsburgh and University of Chicago and used for experiments at 6–8 weeks of age. IFNAR^–/– were housed exclusively at the University of Pittsburgh. RAG^–/– OT-II^+/– CD45.1^+/+ were housed exclusively at the University of Chicago. Intestinal microbiota was controlled in WT and IFNAR^–/– mice by administering only irradiated food and using a water bottle after being weened. Fecal contents or cages were swapped a minimum of 2 times prior to infection. Animal husbandry and experimental procedures were conducted in accordance with Public Health Service policy and approved by the University of Pittsburgh’s and the University of Chicago’s Institutional Animal Care and Use Committees.

Brigleb P.H., Kouame E., Fiske K.L., Taylor G.M., Urbanek K., Medina Sanchez L., Hinterleitner R., Jabri B, & Dermody T.S. (2022). NK cells contribute to reovirus-induced IFN responses and loss of tolerance to dietary antigen. JCI Insight, 7(16), e159823.

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Mouse Strains for Immunological Studies

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B10.A and B10.A.SJL-Rag2^−/− -Ptprc^a-5C.C7(TcRa, TcRb) were obtained from Taconic Farms (Germantown, NY). C57BL/6, C57BL/6 OT-II (B6.Cg- Tg (TcraTcrb) 425Cbn/J, Tram^−/− C57BL/6J- Ticam1Lps2/J, TLR3^−/− B6;129S1- Tlr3tm1Flv/J, MyD88^−/− CBy.129P2(B6) - MyD88tm1Defr/J, IFNAR^−/− B6.129S2- Ifnar1tm1Agt/Mmjax, IL-1R^−/− B6.129S7-Il1r1tm1Imx/J were purchased from Jackson Laboratories (Bar Harbor, ME). All mice were housed under specific pathogen-free animal conditions at the National institute of Allergy and Infectious Diseases (NIAID), and used between 6 and 12 weeks of age in accordance with guidelines provided by the Institutional Animal Care and Use Committee of the NIAID.

Caucheteux S.M., Hu-Li J., Mohammed R., Ager A, & Paul W.E. (2016). Cytokine Regulation of Lung Th17 Response to Airway Immunization using LPS Adjuvant. Mucosal immunology, 10(2), 361-372.

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Ifnar b6.129s2 ifnar1tm1agt mmjax

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6 protocols using ifnar b6.129s2 ifnar1tm1agt mmjax

Generation of Knockout Mouse Strains

Generation of Knockout Mouse Strains

Mouse Strains for Immunological Studies

Evaluation of Knockout Mice in Immune Responses

Controlled Microbiome in Genetically Modified Mice

Mouse Strains for Immunological Studies

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