Log In Sign up
The largest database of trusted experimental protocols

Fumarate assay kit

Manufactured by Merck Group
Visit Supplier
Sourced in United States

The Fumarate Assay Kit is a laboratory tool used to quantify the presence and concentration of fumarate, a key intermediate in the tricarboxylic acid (TCA) cycle. The kit provides a reliable and efficient method for the colorimetric detection and measurement of fumarate levels in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Succinate colorimetric assay kit, by Merck Group (3 mentions) Glucose assay kit, by Solarbio (2 mentions) Micro mitochondrial citric acid content assay kit, by Solarbio (2 mentions) Citroyl synthetase kit, by Nanjing Jiancheng (2 mentions) Citric acid content assay, by Solarbio (2 mentions) Pfkp test kit, by Nanjing Jiancheng (2 mentions) Acetyl coa assay kit, by Solarbio (2 mentions) Atp content assay kit, by Solarbio (2 mentions) La assay kit, by Solarbio (2 mentions) Succinate assay kit, by Merck Group (2 mentions) Malate assay kit, by Merck Group (2 mentions) Alpha ketoglutarate colorimetric fluorometric assay kit, by Abcam (1 mentions) D 2 hydroxyglutarate colorimetric assay kit, by Abcam (1 mentions) Formate assay kit, by Merck Group (1 mentions) Tissuelyser 2, by Qiagen (1 mentions) Lactate assay kit, by Merck Group (1 mentions) Citrate assay kit, by Merck Group (1 mentions) Glutamate assay kit, by Merck Group (1 mentions) α ketoglutarate assay kit, by Merck Group (1 mentions) Bcaa detection kit, by Abcam (1 mentions)

13 protocols using fumarate assay kit

1

Metabolite Quantification Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
α-KG and 2-HG were measured using the alpha-ketoglutarate colorimetric fluorometric assay kit and D-2-hydroxyglutarate colorimetric assay kit (Cat: K677 and K213, Biovision), respectively. Fumarate and succinate were measured using the respective fumarate assay kit and succinate colorimetric assay kits (Cat: MAK060 and MAK184, Sigma).
Shi F., Zhou M., Shang L., Du Q., Li Y., Xie L., Liu X., Tang M., Luo X., Fan J., Zhou J., Gao Q., Qiu S., Wu W., Zhang X., Bode A.M, & Cao Y. (2019). EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter. Theranostics, 9(9), 2424-2438.
+ Open protocol
+ Expand
2

Metabolic Profiling of Cells under Hypoxia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded into six-well plates at a density of 1 × 106 cells in 2 ml RPMI 1640 medium per well and cultured overnight. Cells were then treated with or without 10 μM KU60019 and maintained at 1% O2 for 10–12 h. Glucose consumption, lactate production, ATP production, citrate production, succinate production, fumarate production, and PFKP and CS activity were detected using glucose assay kit (Solarbio® BC2500), LA assay kit (Solarbio® BC2230), ATP content assay kit (Solarbio® BC0300), citric acid (CA) content assay (Solarbio® BC2150), micro mitochondrial citric acid content assay kit (Solarbio® BC2175), succinate colorimetric assay kit (Sigma® MAK184), pyruvate (PA) assay kit (Solarbio® BC2200), acetyl-CoA assay kit (Solarbio® BC0980), fumarate assay kit (Sigma® MAK060), PFKP test kit (Nanjing Jiancheng Bioengineering Institute A129), and citroyl synthetase kit (Nanjing Jiancheng Bioengineering Institute A108) according to instruction of the manufacturers, respectively. All experiments were performed at least three times and the data were normalized by the cell numbers or protein content.
Peng M., Yang D., Hou Y., Liu S., Zhao M., Qin Y., Chen R., Teng Y, & Liu M. (2019). RETRACTED ARTICLE: Intracellular citrate accumulation by oxidized ATM-mediated metabolism reprogramming via PFKP and CS enhances hypoxic breast cancer cell invasion and metastasis. Cell Death & Disease, 10(3), 228.
+ Open protocol
+ Expand
3

Biomass and Metabolite Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biomass concentration was determined both by counting the number of cells per milliliter, as previously described (81 (link)), and by the DCW method after centrifugation (16,000 × g, 5 min, room temperature), two washes with Milli-Q water, and drying under vacuum at 80°C. The concentrations of glucose, glycerol, acetate, butyrate, lactate, pyruvate, acetoin, acetone, ethanol, and butanol were determined based on high-performance liquid chromatography (HPLC), as described by Dusséaux et al. (82 (link)), except that the concentration of H2SO4 was changed to 0.5 mM, as required for mobile phase optimization. The concentrations of formate and fumarate were measured using a formate assay kit (Sigma-Aldrich) and a fumarate assay kit (Sigma-Aldrich), according to the manufacturer’s instructions.
Yoo M., Bestel-Corre G., Croux C., Riviere A., Meynial-Salles I, & Soucaille P. (2015). A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum. mBio, 6(6), e01808-15.
+ Open protocol
+ Expand
4

Measuring Metabolic Markers in LO2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The AcAc content in LO2 cells was measured by human acetoacetate ELISA Kit (Shanghai J&L Biological, China). The succinate and fumarate content of LO2 cells were measured using the Succinate Assay Kit (Sigma-Aldrich, Saint Louis, MO, USA) and the Fumarate Assay Kit (Sigma-Aldrich, Saint Louis, MO, USA) according to the manufacturer’s instructions.
Xu B.T., Teng F.Y., Wu Q., Wan S.R., Li X.Y., Tan X.Z., Xu Y, & Jiang Z.Z. (2022). Bdh1 overexpression ameliorates hepatic injury by activation of Nrf2 in a MAFLD mouse model. Cell Death Discovery, 8, 49.
+ Open protocol
+ Expand
5

Fumarate Quantification in Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fumarate was measured in serum as described in the manufacturer’s protocol using the Fumarate Assay Kit (Sigma-Aldrich, St. Louis, MO). Fumarate contents were normalized to the serum volume (50 μl) used in experiments.
Katsuta N., Nagai M., Saruwatari K., Nakamura M, & Nagai R. (2023). Mitochondrial stress and glycoxidation increase with decreased kidney function. Journal of Clinical Biochemistry and Nutrition, 72(2), 147-156.
+ Open protocol
+ Expand
6

Metabolite Quantification in Knee Joints

Check if the same lab product or an alternative is used in the 5 most similar protocols
The knee joints, excluding the excessive surrounding tissues, were collected into individual Eppendorf tubes containing 1 mL of sterile PBS, followed by homogenization with TissueLyser II (Qiagen, Hilden, Germany). Afterward, the samples were centrifuged (11,000 rpm for 10 min at 4°C), and the supernatant was processed following metabolite extraction and sample preparation for assessment of metabolite expression. Lactate, fumarate, and malate were measured by using lactate assay kit (catalog no. MAK064; Sigma, Germany), fumarate assay kit (catalog no. MAK060, Sigma, Germany), and malate assay kit (catalog no. MAK067, Sigma, Germany) according to the manufacturer’s instructions.
Nguyen M.T., Hu Z., Mohammad M., Schöttler H., Niemann S., Schultz M., Barczyk-Kahlert K., Jin T., Hayen H, & Herrmann M. (2023). Bacterial Lipoproteins Shift Cellular Metabolism to Glycolysis in Macrophages Causing Bone Erosion. Microbiology Spectrum, 11(3), e04293-22.
+ Open protocol
+ Expand
7

Quantification of Fumarate Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stationary-phase cells were resuspended in M9 with or without 15 mM fumarate. For each time point, samples were collected and centrifuged at high speed, and the supernatant was passed through a 0.2 μm cellulose acetate filter. The filtered supernatants were then quantified using a fumarate assay kit (Sigma-Aldrich). Experiments were performed in triplicate.
Meylan S., Porter C.B., Yang J.H., Belenky P., Gutierrez A., Lobritz M.A., Park J., Kim S.H., Moskowitz S.M, & Collins J.J. (2017). Carbon Sources Tune Antibiotic Susceptibility in Pseudomonas aeruginosa via Tricarboxylic Acid Cycle Control. Cell chemical biology, 24(2), 195-206.
+ Open protocol
+ Expand
8

Metabolic Profiling of Cells under Hypoxia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded into six-well plates at a density of 1 × 106 cells in 2 ml RPMI 1640 medium per well and cultured overnight. Cells were then treated with or without 10 μM KU60019 and maintained at 1% O2 for 10–12 h. Glucose consumption, lactate production, ATP production, citrate production, succinate production, fumarate production, and PFKP and CS activity were detected using glucose assay kit (Solarbio® BC2500), LA assay kit (Solarbio® BC2230), ATP content assay kit (Solarbio® BC0300), citric acid (CA) content assay (Solarbio® BC2150), micro mitochondrial citric acid content assay kit (Solarbio® BC2175), succinate colorimetric assay kit (Sigma® MAK184), pyruvate (PA) assay kit (Solarbio® BC2200), acetyl-CoA assay kit (Solarbio® BC0980), fumarate assay kit (Sigma® MAK060), PFKP test kit (Nanjing Jiancheng Bioengineering Institute A129), and citroyl synthetase kit (Nanjing Jiancheng Bioengineering Institute A108) according to instruction of the manufacturers, respectively. All experiments were performed at least three times and the data were normalized by the cell numbers or protein content.
Peng M., Yang D., Hou Y., Liu S., Zhao M., Qin Y., Chen R., Teng Y, & Liu M. (2019). RETRACTED ARTICLE: Intracellular citrate accumulation by oxidized ATM-mediated metabolism reprogramming via PFKP and CS enhances hypoxic breast cancer cell invasion and metastasis. Cell Death & Disease, 10(3), 228.
+ Open protocol
+ Expand
9

Retinal Fumarate Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
The total fumarate content per retina was measured using the Fumarate Assay Kit (Sigma‐Aldrich) according to the manufacturer's instructions.
Izuta Y., Imada T., Hisamura R., Oonishi E., Nakamura S., Inagaki E., Ito M., Soga T, & Tsubota K. (2017). Ketone body 3‐hydroxybutyrate mimics calorie restriction via the Nrf2 activator, fumarate, in the retina. Aging Cell, 17(1), e12699.
+ Open protocol
+ Expand
10

Intracellular Metabolite Profiling of ADSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
ADSCs (2 × 106 in quantity) transfected with shNC or shBcat1#1 were used for intracellular metabolite concentration measurement. Each metabolite level was determined using the following assay kits: BCAA detection kit (BioVision, K564‐100, α‐ketoglutarate assay kit (Sigma‐Aldrich, MAK054), glutamate assay kit (Sigma‐Aldrich, MAK004), citrate assay kit (Sigma‐Aldrich, MAK057), fumarate assay kit (Sigma‐Aldrich, MAK060), succinate assay kit (Sigma‐Aldrich, MAK184), and malate assay kit (Sigma‐Aldrich, MAK196). BCKA concentration was measured by high‐performance liquid chromatography (HPLC) as previously described.[42] Intracellular metabolite levels were normalized to the ADSCs transfected with shNC.
Hu G., Cui Z., Chen X., Sun F., Li T., Li C., Zhang L., Guo X., Zhao H., Xia Y., Yan W., Yi W., Fan M., Yang R., Wang S., Tao L, & Zhang F. (2023). Suppressing Mesenchymal Stromal Cell Ferroptosis Via Targeting a Metabolism‐Epigenetics Axis Corrects their Poor Retention and Insufficient Healing Benefits in the Injured Liver Milieu. Advanced Science, 10(13), 2206439.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!