Anti cd138 fitc m15

Lenalidomide was purchased from (BioVision Inc.), Pomalidomide and Phthalimide were purchased from (Sigma-Aldrich). These drugs were dissolved in dimethylsulphoxide (DMSO) and stored at −20°C until use. The final concentration of DMSO in all experiments was < 0.1%.
The following monoclonal antibodies (mAbs) were used for immunostaining or as blocking Abs: anti-MICA (MAB159227), anti-MICB (MAB236511), anti-ULBP-1 (MAB170818), anti-ULBP-2/5/6 (MAB165903), anti-ULBP-3 (MAB166510) and anti-NKG2D (MAB149810) from (R&D System), anti-PVR/CD155 (SKII.4) kindly provided by Prof. M. Colonna (Washington University, St Louis, MO), anti-CD56 (C218) mAb was provided by Dr. A. Moretta (University of Genoa, Genoa, Italy), anti-DNAM-1 (DX11) from (Serotec), APC Goat anti-mouse IgG (Poly4053), anti-CD3/APC (HIT3a), anti-CD56/PE (HCD56), mouse IgG1/FITC, /PE or /APC isotype control (MOPC-21) were purchased from (BioLegend). Anti-CD107a/FITC (H4A3), anti-CD138-FITC (M15) and anti-CD38-APC (HIT2) were purchased from (BD Biosciences).

The bromodomain inhibitor JQ1 and Bortezomib (PS-341) were purchased from Selleckchem.Com (Munich, Germany). Lenalidomide was purchased from BioVision Inc. (Milpitas, California USA). The hetero-bifunctional PROTAC (Proteolysis Targeting Chimera) ARV-825, was purchased from Chemieteck (Indianapolis, IN, USA). These drugs were dissolved in dimethylsulphoxide (DMSO) and stored at −20 °C until use. The final concentration of DMSO in all experiments was < 0.1%. Roscovitine a potent and selective inhibitor of cyclin-dependent kinases and the antibiotic Doxycycline hydrate (used to induce SKO-007(J3)/shMEIS2-Tet cells), Melphalan and 17-AAG (17-allylaminogeldanamycin) were purchased from Sigma-Aldrich S.r.l. (Milan, IT). The following monoclonal antibodies (mAbs) were used for immunostaining or as blocking Abs: anti-MICA (MAB159227) was purchased from R&D System (Minneapolis, MN), anti-PVR/CD155 (SKII.4) kindly provided by Prof. M. Colonna (Washington University, St Louis, MO), APC Goat anti-mouse IgG (Poly4053), was purchased from BioLegend (San Diego, CA). Anti-CD138-FITC (M15) and anti-CD38-APC (HIT2) were purchased from BD Biosciences (San Jose, CA).