After 22 h ZnSO4, ZnO NPs, CuO NPs and SiO2 NPs treatment, a Click-iT 5-ethynyl-2′-deoxyuridine (EdU) kit (Molecular Probes, Carlsbad, CA, USA) was used to measure cell proliferation according to the manufacturer's procedures [124 (link)]. Cells were labeled with EdU (10 μmol/L) for 2 h. After fixation (4% paraformaldehyde, 60 minutes) and transparency (0.5% Triton X-l00, 30 minutes) treatment, the cells were incubated with Click-iT® reaction cocktail, followed by 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining. After rinsing three times, cells were observed under an inverted fluorescence microscope with three random fields of view. The stained sections were visualized with a Nikon Eclipse TE2000-U fluorescence microscope (Nikon, Inc., Melville, NY), and the captured fluorescent images were analyzed using MetaMorph software [118 (link)].
Click it 5 ethynyl 2 deoxyuridine edu kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Click-iT 5-ethynyl-2'-deoxyuridine (EdU) kit is a tool used for labeling and detecting DNA synthesis. It incorporates the EdU nucleoside analog into newly synthesized DNA, which can then be detected using a copper-catalyzed click reaction.
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Lab products found in correlation
2 protocols using click it 5 ethynyl 2 deoxyuridine edu kit
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Cell Proliferation Assay with EdU Staining
2
Cell Proliferation Assay Using EdU
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After 72 hours of virus infection, BLS cells were seeded in 96-well plates. A Click-iT 5-ethynyl-2′-deoxyuridine (EdU) kit (Molecular Probes, Carlsbad, CA, USA) was used to measure cell proliferation according to the manufacturer’s procedures. Cells were labeled with EdU. Culture medium (100 µL) containing EdU (5 mol/L) was added to each well, followed by a 2-hour incubation. After fixation (4% paraformaldehyde [Shanghai biotechwell Co Ltd, Shanghai, People’s Republic of China], 30 minutes) and transparency (0.5% Triton X-l00 [Sigma-Aldrich Co., St Louis, MO, USA], 10 minutes) treatment, l00 µL × Hoechst 33342 reaction solution (Sigma-Aldrich Co) was added to each well, followed by 4′,6-diamidino-2-phenylindole nuclear staining. After rinsing three times, cells were observed under an inverted fluorescence microscope with three random fields of view. All images were obtained and processed with ImagePro software (Media Cybernetics, Rockville, MD, USA).
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