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Milliplex mouse cytokine chemokine 22 plex kit

Manufactured by Merck Group

The Milliplex Mouse Cytokine/Chemokine 22-plex kit is a multiplex assay designed for the simultaneous quantitative measurement of 22 different cytokines and chemokines in mouse biological samples, such as cell culture supernatants, serum, and plasma. The kit utilizes Luminex technology to provide a comprehensive analysis of the mouse immune system.

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2 protocols using milliplex mouse cytokine chemokine 22 plex kit

1

Cytokine and Chemokine Profiling

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Most cytokines and chemokines were measured in sera or clarified liver homogenates taken at 24 & 72 h by the Milliplex Mouse Cytokine/Chemokine 22-plex kit (Millipore #MPXMCYTO70KPMX22) on the Luminex 200 system. Serum IFN-α and IFN-β levels at 24 h were measured using the VeriKine Mouse IFN Alpha ELISA kit and VeriKine Mouse IFN Beta ELISA kit (R&D Systems), respectively, as per manufacturer’s instructions.
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2

Multiplex Cytokine/Chemokine Profiling

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Cytokines and chemokines were measured from sera taken on days 1 and 3 post-infection using the Milliplex mouse cytokine/chemokine 22-plex kit (Millipore #MPXMCYTO70KPMX22) with the Luminex 200 system. G-CSF (R&D Systems), IL-10 (BD Biosciences), IL-6, TNF-α, IFN-γ, and MCP-1 (Biolegend) were measured in the sera of the Z-VAD-FMK and vehicle pre-treated mice by ELISA assay. Data are expressed as mean pg/ml ± SEM.
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