Anti gata 1 n6

A ChIP assay was performed as in a prior report [47 (link)] using anti-GATA-1 (N6; Santa Cruz), anti-GATA2 (B9922A; Perseus Proteomics, Tokyo, Japan), anti-CTCF (A300-543A; Bethyl, Montgomery, TX, USA) and anti-Rad21 (Ab992; Abcam, Cambridge, UK) antibodies and normal Rabbit IgG (2729S; Cell Signaling Technology, Danvers, MA, USA) and anti-Rat IgG (sc-2026; Santa Cruz). As described previously, the GATA1 ChIP assay was done using an anti-rat IgG rabbit antibody (Jackson ImmunoResearch, West Grove, PA, USA) as a secondary antibody to precipitate the immune complex [47 (link)]. The DNA purified from ChIP samples were amplified was analyzed using an Mx3000P real-time PCR system (Agilent) with the GoTaq qPCR Master Mix (Promega). The primer sequences used are shown in Table A2.

The whole lysates were prepared as previously described (ref). The samples were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the Western blot analyses were performed as described previously [47 (link)] using anti-GATA1 (N6; Santa Cruz, Dallas, TX, USA), anti-GATA2 (H-116; Santa Cruz), anti-MCP6 (sc-32474; Santa Cruz), anti-MITF (H-50; Santa Cruz), and anti-lamin B (M-20; Santa Cruz) antibodies.