Cd68 clone y1 82a

Manufactured by BioLegend

Sourced in United States

CD68 (clone Y1/82A) is a mouse monoclonal antibody that recognizes the CD68 antigen, a glycoprotein expressed primarily on the lysosomal membranes of monocytes and macrophages. This antibody can be used to identify and characterize these cell types in various applications.

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Lab products found in correlation

Image pro plus software, by Media Cybernetics (2 mentions) Goat serum, by Agilent Technologies (1 mentions) Cd45 clone hi30, by BioLegend (1 mentions) Cd163 clone ghi 61, by BioLegend (1 mentions) Vimentin clone d21h3, by Cell Signaling Technology (1 mentions) Vectashield containing dapi, by Vector Laboratories (1 mentions) Biotin blocking system, by Agilent Technologies (1 mentions) Ds fi1 digital camera, by Nikon (1 mentions) Caspase 1, by Merck Group (1 mentions) Eclipse 80i microscope, by Nikon (1 mentions) Vectashield containing 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions)

3 protocols using cd68 clone y1 82a

Immunophenotyping Synovial Tissue Sections

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Frozen synovial tissue sections were fixed in acetone and blocked with 10% goat serum (Dako), followed by incubation with the Biotin blocking system (Dako). Stainings with mAb directed against TNF (clone 52B83; Hycult Biotech), CD45 (clone HI30; BioLegend), CD55 (clone JS11; BioLegend), CD68 (clone Y1/82A; BioLegend), CD90 (clone 5E10; BioLegend), CD163 (clone GHI/61; BioLegend), and vimentin (clone D21H3; Cell Signaling Technology) were performed overnight at 4°C, followed by incubation with Alexa Fluor 488/Alexa Fluor 555–conjugated goat anti-mouse and goat anti-rabbit secondary antibodies. Slides were mounted with Vectashield containing DAPI (Vector Laboratories) and analyzed on a fluorescence imaging microscope (Leica DMRA) coupled to a charge-coupled device camera, with results analyzed using Image-Pro Plus software (Media Cybernetics, Dutch Vision Components).

Kaaij M.H., van Tok M.N., Blijdorp I.C., Ambarus C.A., Stock M., Pots D., Knaup V.L., Armaka M., Christodoulou-Vafeiadou E., van Melsen T.K., Masdar H., Eskes H.J., Yeremenko N.G., Kollias G., Schett G., Tas S.W., van Duivenvoorde L.M, & Baeten D.L. (2020). Transmembrane TNF drives osteoproliferative joint inflammation reminiscent of human spondyloarthritis. The Journal of Experimental Medicine, 217(10), e20200288.

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Immunohistochemical Analysis of Chronic Wounds

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Cryosections (10-μm thick) were cut from human chronic wound biopsies, fixed in cold acetone and blocked with buffer containing 3% BSA. Sections were then incubated overnight with primary antibodies against CD68 (clone Y1/82A, 1:100; Biolegend) and caspase-1 (Millipore). Sections were then incubated with FITC- and tetramethylrhodamine isothiocyanate-conjugated isotype-specific secondary antibodies (1:200; Invitrogen). Negative controls included nonstained slides and isotype-specific control antibodies (IgG1 and IgG2b; Biolegend) along with secondary antibodies. Digital images were obtained using a Nikon Instruments Eclipse 80i microscope with a ×20/0.75 objective, a DS-Fi1 digital camera, and NIS Elements software.

Mirza R.E., Fang M.M., Weinheimer-Haus E.M., Ennis W.J, & Koh T.J. (2014). Sustained Inflammasome Activity in Macrophages Impairs Wound Healing in Type 2 Diabetic Humans and Mice. Diabetes, 63(3), 1103-1114.

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Immunolocalization of IL-23-Positive Cells

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Frozen synovial biopsy tissue sections were used for double staining of IL-23-positive cells. The staining was performed with monoclonal mouse anti-human IL-23 p19 (clone eBio473P19, eBioscience, San Diego, CA, USA) in combination with biotinylated monoclonal antibodies against macrophages (CD68, clone Y1/82A, Biolegend, San Diego, CA, USA) and stromal cells (vimentin, (D21H3) XP™ rabbit monoclonal antibody, Cell Signaling Technology, Danvers, MA, USA). Incubation with the primary antibodies was carried out overnight at 4 °C, followed by incubation with a secondary Alexa Fluor 488-conjugated goat anti-mouse antibody and streptavidin-Alexa Fluor 594 (in case of staining with CD68) and secondary Alexa Fluor 594-conjugated goat anti-rabbit antibody (in case of staining with vimentin) was used. Slides were mounted with Vectashield containing 4′,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories) and analyzed on a fluorescent imaging microscope (Leica DMRA, Wetzlar, Germany) coupled to a CCD camera and Image-Pro Plus software (Media Cybernetics, Dutch Vision Components, Breda, the Netherlands).

Cañete J.D., Celis R., Yeremenko N., Sanmartí R., van Duivenvoorde L., Ramírez J., Blijdorp I., García-Herrero C.M., Pablos J.L, & Baeten D.L. (2015). Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis. Arthritis Research & Therapy, 17(1), 173.

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