Chromatography grade methanol

Flavone (PHE, purity > 99%), 3-Hydroxyflavonol (FLV, purity > 95%), Chrysin (CHY, purity > 98%), Baicalein (BAC, purity > 98%), Luteolin (LUT, purity > 98%), Quercetin (QUR, purity > 98%), and Myricetin (MYR, purity > 98%) were acquired from Beijing Coupling Technology Co. Ltd. (Shanghai, China). Poloxamer 188 (P188, purity > 99%) was acquired from Shanghai, China. Methanol (purity > 99.5%), anhydrous ethanol (purity > 99.7%), and acetone (purity > 99%) were procured from Tianjin Daimao Chemical Reagent Technology Co. Ltd. (Tianjin, China). Chromatography-grade methanol (chromatographic grade) was acquired from Merck Co., Ltd. (Darmstadt, Germany). Chemical structures and pertinent physical and chemical characteristics of the drugs and polymers employed in our experiments are highlighted in Figure 1.

Black beans were purchased from Jiansanjiang Farm, Heilongjiang Province, China. Food-grade lactic acid was purchased from Zhengzhou Gaoyan Biotechnology Co., Ltd. (Zhengzhou, China). R. oligosporus starter powder was purchased from Nanjing Tianbeiren Fermentation Technology Co., Ltd. (Nanjing, China). Chromatography-grade methanol, trifluoroacetic acid (TFA), and acetonitrile were purchased from Merck (Darmstadt, Germany). Water-soluble vitamin E (Trolox), 1,1-diphenyl-2-picrylhydrazyl (DPPH), pepsin, trypsin, salivary amylase, angiotensin I-converting enzyme (ACE), and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from Sigma-Aldrich Chemical Co., Ltd. (St. Louis, MO, USA). All other chemicals and reagents were of analytical grade and purchased from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China).

Indantrione hydrate, stannous chloride and anthrone were purchased from Macklin biotechnology (Shanghai, China). Chromatography-grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany). Formic acids (≥96%), acetic acid (≥96%) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Authentic standards were purchased from Sigma-Aldrich (St Louis, MO, United States), Yuanye bio-technology (Shanghai, China), Jinsui biotechnology (Shanghai, China), J&K scientific (Beijing, China) and ZZBIO (Shanghai, China). Diagnostic solution for electronic tongue correction was purchased from Evensen Technology Co., Ltd. (Tianjin, China).

The 60-mer single A-containing
DNA (single A-DNA), single 6mA-containing DNA (single 6mA-DNA), and
single I-containing DNA (single I-DNA) were purchased from Sangon
Biotech (Shanghai, China). The sequences of these oligonucleotides
are listed in Table S1. 2′-Deoxycytidine
(dC), 2′-deoxyguanosine (dG), 2′-deoxyadenosine (dA),
thymidine (dT), 2′-deoxyinosine (dI), and phosphodiesterase
I were purchased from Sigma-Aldrich (St. Louis, MO, USA). N⁶-Methyl-2′-deoxyadenosine (6mA) and
5-methyl-2′-deoxycytidine (5mdC) were purchased from Berry
& Associates (Dexter, MI, USA). DNase I, calf intestine alkaline
phosphatase (CIAP), and TB Green Premix Ex TaqII
(Tli RNaseH Plus) were purchased from Takara Biotechnology Co., Ltd.
(Dalian, China). NEBNext Multiplex Oligos for Illumina (Index Primers
Set 1) were purchased from New England Biolabs (Ipswich, MA, USA).
Chromatography-grade methanol was purchased from Merck (Darmstadt,
Germany). All other solvents and chemicals were of analytical grade.

Camellia oil (acid value (AV)_CO:1.10 mg/g, AV_MRO:0.58 mg/g, AV_RO:0.17 mg/g) was offered by Ganzhou Jinxi Agricultural Development Co., Ltd. (Ganzhou, China). Standard α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, and fatty acid methyl ester were obtained from Tianjin Alta Technology Co., Ltd. (Tianjin, China). Chromatography-grade methanol, hexane, acetonitrile, dichloromethane, and methylene chloride were obtained from Merck Life Sciences (Shanghai, China) Co., Ltd. (Shanghai, China). All other chemicals used were of analytical grade.